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OPPORTUNISTIC MICROORGANISMS IN INJURIES denture stomatitis AND INFLUENCE OF PHOTODYNAMIC THERAPY IN VIRULENCE FACTORS OF Candida spp.

Grant number: 10/00879-4
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2010
Effective date (End): February 28, 2013
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:Antonio Olavo Cardoso Jorge
Grantee:Cristiane Aparecida Pereira
Home Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Estadual Paulista (UNESP). Campus de São José dos Campos. São José dos Campos , SP, Brazil

Abstract

The aims of this study will be to isolate and identify opportunistic microorganisms in lesions of denture stomatitis (DS) and verify the production of virulence factors by Candida albicans, Candida dubliniensis, Candida glabrata and Candida tropicalis; evaluate the effects of photodynamic therapy (PDT) on planktonic cultures and biofilms formed by these yeasts. In addition, the effect of PDT will be assessed on the virulence factors of these yeasts. 50 patients with DS and 50 individuals without DS will be examined. The samples from oral cavity will be collected with sterile swabs and through rinse mouth. Samples will be seeded onto selective agar and the organisms will be identified biochemically by the API® system. For C. dubliniensis, this identification will also be performed using the technique of Polymerase Chain Reaction (PCR). Isolates identified as C. albicans, C. dubliniensis, C. glabrata and C. tropicalis will be assessed for the production of the following virulence factors: secretion of the enzyme phospholipase, proteinase, chondroitinase, lipase, hemolysin; cell surface hydrophobicity; germ tube production; and biofilm formation. The assays of PDT will be performed in planktonic cultures, and in biofilms formed by the Calgary Biofilm Device (CBD), with strains of C. albicans, C. glabrata, C. tropicalis and the isolates of C. dubliniensis that have higher and lower expression of virulence factors. A green light emitting diode (532 ± 10 nm) with the output power of 80 mW will be used as a light source and erythrosine as photosensitizer. The number of colony-forming units per milliliter (CFU/mL) in Log10 will be analyzed statistically (p <0.05). Scanning electron microscopy and confocal laser scanning microscopy on discs treated with PDT and control biofilms groups will be performed. After testing of PDT in planktonic and biofilm cultures of C. albicans, C. dubliniensis, C. glabrata and C. tropicalis, the phenotypic expression of the virulence factors will be evaluated in yeast remaining again.

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Scientific publications (13)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PEREIRA, CRISTIANE APARECIDA; DOMINGUES, NADIA; SILVA, MICHELLE PENELUPPI; BORGES PEREIRA COSTA, ANNA CAROLINA; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Photodynamic inactivation of virulence factors of Candida strains isolated from patients with denture stomatitis. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 153, p. 82-89, . (11/21346-7, 10/00879-4, 10/08936-7, 13/07411-6)
COELHO OLIVEIRA, SARAH ALMEIDA; MINA ZAMBRANA, JESSICA RABELO; REIS DI IORIO, FERNANDA BISPO; PEREIRA, CRISTIANE APARECIDA; CARDOSO JORGE, ANTONIO OLAVO. The antimicrobial effects of Citrus limonum and Citrus aurantium essential oils on multi-species biofilms. Brazilian Oral Research, v. 28, n. 1, p. 22-27, . (09/52048-1, 10/00879-4)
COSTA, ANNA C. B. P.; PEREIRA, CRISTIANE A.; FREIRE, FERNANDA; JUNQUEIRA, JULIANA C.; JORGE, ANTONIO O. C.. Methods for obtaining reliable and reproducible results in studies of Candida biofilms formed in vitro. MYCOSES, v. 56, n. 6, p. 614-622, . (11/21346-7, 10/00879-4, 12/09188-0, 10/18753-7)
PEREIRA, CRISTIANE APARECIDA; DOMINGUES, NADIA; SANTOS ALVES ARAUJO, MARIA IZABEL DANIEL; JUNQUEIRA, JULIANA CAMPOS; BACK-BRITO, GRAZIELLA NUERNBERG; CARDOSO JORGE, ANTONIO OLAVO. Production of virulence factors in Candida strains isolated from patients with denture stomatitis and control individuals. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 85, n. 1, p. 66-72, . (10/00879-4, 10/08936-7, 13/07411-6)
COSTA, ANNA C. B. P.; PEREIRA, CRISTIANE A.; JUNQUEIRA, JULIANA C.; JORGE, ANTONIO O. C.. Recent mouse and rat methods for the study of experimental oral candidiasis. VIRULENCE, v. 4, n. 5, p. 391-399, . (11/21346-7, 10/00879-4, 10/19117-7, 10/18753-7)
FREIRE, FERNANDA; BORGES PEREIRA COSTA, ANNA CAROLINA; PEREIRA, CRISTIANE APARECIDA; BELTRAME JUNIOR, MILTON; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Comparison of the effect of rose bengal- and eosin Y-mediated photodynamic inactivation on planktonic cells and biofilms of Candida albicans. Lasers in Medical Science, v. 29, n. 3, p. 949-955, . (11/21346-7, 10/00879-4, 09/52048-1, 10/12115-9)
PEREIRA, CRISTIANE APARECIDA; TOLEDO, BRUNA COSTA; SANTOS, CAMILA TELES; BORGES PEREIRA COSTA, ANNA CAROLINA; BACK-BRITO, GRAZIELLA NUERNBERG; KAMINAGAKURA, ESTELA; CARDOSO JORGE, ANTONIO OLAVO. Opportunistic microorganisms in individuals with lesions of denture stomatitis. DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, v. 76, n. 4, p. 419-424, . (11/21346-7, 10/00879-4, 10/08936-7)
PEREIRA, C. A.; ESKELSON, E.; CAVALLI, V.; LIPORONI, P. C. S.; JORGE, A. O. C.; DO REGO, M. A.. Streptococcus mutans Biofilm Adhesion on Composite Resin Surfaces After Different Finishing and Polishing Techniques. Operative Dentistry, v. 36, n. 3, p. 311-317, . (10/00879-4)
PEREIRA, CRISTIANE APARECIDA; ROMEIRO, ROGERIO LIMA; BORGES PEREIRA COSTA, ANNA CAROLINA; SILVA MACHADO, ANA KARINA; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Susceptibility of Candida albicans, Staphylococcus aureus, and Streptococcus mutans biofilms to photodynamic inactivation: an in vitro study. Lasers in Medical Science, v. 26, n. 3, p. 341-348, . (10/00879-4, 09/12005-1, 09/52048-1)
PEREIRA, CRISTIANE APARECIDA; BORGES PEREIRA DA COSTA, ANNA CAROLINA; SILVA MACHADO, ANA KARINA; BELTRAME JUNIOR, MILTOM; AMORIN COSTA ZOELLNER, MARIA STELLA; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Enzymatic Activity, Sensitivity to Antifungal Drugs and Baccharis dracunculifolia Essential Oil by Candida Strains Isolated from the Oral Cavities of Breastfeeding Infants and in Their Mothers' Mouths and Nipples. Mycopathologia, v. 171, n. 2, p. 103-109, . (10/00879-4, 09/12005-1)
BORGES PEREIRA COSTA, ANNA CAROLINA; CAMPOS RASTEIRO, VANESSA MARIA; HALTER DA SILVA HASHIMOTO, EMILY SETSUKO; ARAUJO, CASSIA FERNANDES; PEREIRA, CRISTIANE APARECIDA; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Effect of erythrosine- and LED-mediated photodynamic therapy on buccal candidiasis infection of immunosuppressed mice and Candida albicans adherence to buccal epithelial cells. ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY, v. 114, n. 1, p. 67-74, . (09/12005-1, 10/00879-4, 10/01988-1, 09/52048-1, 10/18753-7)
PEREIRA, CRISTIANE APARECIDA; BORGES PEREIRA COSTA, ANNA CAROLINA; CARREIRA, CLAUDIA MOURA; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAVO. Photodynamic inactivation of Streptococcus mutans and Streptococcus sanguinis biofilms in vitro. Lasers in Medical Science, v. 28, n. 3, p. 859-864, . (11/21346-7, 10/00879-4, 09/52048-1)
BORGES PEREIRA COSTA, ANNA CAROLINA; DE CAMPOS RASTEIRO, VANESSA MARIA; PEREIRA, CRISTIANE APARECIDA; HALTER DA SILVA HASHIMOTO, EMILY SETSUKO; BELTRAME JUNIOR, MILTON; JUNQUEIRA, JULIANA CAMPOS; CARDOSO JORGE, ANTONIO OLAUO. Susceptibility of Candida albicans and Candida dubliniensis to erythrosine- and LED-mediated photodynamic therapy. ARCHIVES OF ORAL BIOLOGY, v. 56, n. 11, p. 1299-1305, . (09/12005-1, 10/00879-4, 10/01988-1, 10/08936-7, 09/52048-1, 10/18753-7)

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