Hybridization between different species of fish has been performed artificially in order to produce advantageous fishes. However, this practice can lead to serious problems such as genetic contamination of cultivated stocks, escapes of hybrids into the natural environment, genetic introgression and even the genetic extinction of parental species. Hybrids between Pseudoplatystoma corruscans (pintad) and Pseudoplatystoma reticulatum (cachara) has been produced in several fish farms in Brazil, which, in captivity, may have fertility, and backcross with both pure species. The identification of parental lines and F1 or post F1 hybrid strains is crucial for the development of a proper monitoring and management of these individuals in fish farms, as well for a monitoring of the natural environment, avoiding the risks that hibridization represent to the environment. This study aim to obtain molecular markers for a quickly and precise identification of hybrids involving the species P. corruscans and P. reticulatum. These markers will be usefull for the monitoring of the natural environment, allowing an assessment of the actual benefits and risks that may result from the production of hybrid and provide subsidies for projects of protection and conservation of pure parental species.
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