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Multiresidue analysis of pesticides in tomato using LC-MS/MS and evaluation the effects of washing in decontamination.

Grant number: 09/15816-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2010
Effective date (End): December 31, 2012
Field of knowledge:Agronomical Sciences - Food Science and Technology
Principal Investigator:Valdemar Luiz Tornisielo
Grantee:Graziela Cristina Rossi Moura Andrade
Host Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil


Pesticides have been widely used in agriculture to control pests, diseases and weed. Although the use of these substances brings benefits to society by increasing food availability, negative aspects are associated with misuse such as soil contamination, water and food. The tomato is a vegetable very consumed, and beyond their basic nutritional qualities, is considered a functional food bringing health benefits. The intensive use of pesticides on crops of tomatoes in breach of the good agricultural practices have caused concerns about possible contamination of the final product and many multiresidue methods have been employed to evaluate and determine the residues levels in food samples. Therefore, the aim this study is the validation of the method of sample preparation QuEChERS for quantification of residues of 50 pesticides of chemical classes different in tomato, determination of residue levels in commercial samples, evaluation of profile distribution of pesticide residues in cuticular and subcuticular tissues of tomato, as well as the evaluation of residues in whole samples without washing and after 2 washing procedures. The detection will be performed using LC-MS/MS with electrospray interface system, operated in positive mode ionization.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ANDRADE, G. C. R. M.; MONTEIRO, S. H.; FRANCISCO, J. G.; FIGUEIREDO, L. A.; BOTELHO, R. G.; TORNISIELO, V. L.. Liquid chromatography-electrospray ionization tandem mass spectrometry and dynamic multiple reaction monitoring method for determining multiple pesticide residues in tomato. Food Chemistry, v. 175, p. 57-65, . (09/15816-0, 10/18316-6)
ANDRADE, GRAZIELA C. R. M.; MONTEIRO, SERGIO H.; FRANCISCO, JEANE G.; FIGUEIREDO, LEILA A.; ROCHA, ADERBAL A.; TORNISIELO, VALDEMAR L.. Effects of Types of Washing and Peeling in Relation to Pesticide Residues in Tomatoes. Journal of the Brazilian Chemical Society, v. 26, n. 10, p. 1994-2002, . (09/15816-0, 10/18316-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ANDRADE, Graziela Cristina Rossi Moura. Multiresidue analysis of pesticides in tomatoes using LC-MS/MS and evaluation of the effects of washing in decontaminating. 2013. Doctoral Thesis - Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB) Piracicaba.

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