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Efficacy of Melaleuca alternifolia on biofilm cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus

Grant number: 09/11939-0
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2010
Effective date (End): September 30, 2011
Field of knowledge:Health Sciences - Dentistry
Principal researcher:Denise Madalena Palomari Spolidorio
Grantee:Amanda Fontana
Home Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


The reduction of specific pathogens associated with caries through agents with extracts or compounds isolated from medicinal plants may be an alternative treatment for control of cariogenic biofilm. Due to its bactericidal action, evidence suggests that the oil of Melaleuca alternifolia (TTO) may be helpful in maintaining oral hygiene and the prevention of dental diseases. Objective: To investigate the effect of M. alternifolia in biofilms of cariogenic bacteria, S. mutans and L. acidophilus. Material and Methods: The first phase of this work is determination of minimum inhibitory Tconcentration (MIC) and minimum bactericidal concentration (MBC) of TTO for S. mutans and L. acidophilus. The antimicrobial effect of TTO will be analyzed on SSB (monoespécie) and DSB (multispecies) the next steps. The second phase will examine various concentrations of TTO on biofilm SSB and DSB formed on the surfaces of wells cell culture plate by the MTT technique and number of CFU / ml. In the third phase, different contact times (15s, 30, 60s) of TTO on biofilm SSB and DSB in enamel blocks will be analyzed. The gold standard, we will use 0.12% chlorhexidine repeating the same procedure. For analysis of the third phase will use number of CFU / ml, confocal laser scanning (MCVL) to observe the cells viable and nonviable biofilm and scanning electron microscopy (SEM) for the exploration of the viability of the cells biofilm.

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Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
FONTANA, Amanda. Eficácia do óleo de Melaleuca alternifolia sobre bactérias cariogênicas e sua citoxicidade sobre cultura de queratinócitos. 2011. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

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