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Construction of a protease inhibitor library in phage display system and its selection of specific inhibitors for digestive proteases of Aedes aegypti

Grant number: 09/06003-6
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): September 01, 2009
Effective date (End): August 31, 2012
Field of knowledge:Biological Sciences - Biochemistry - Chemistry of Macromolecules
Principal Investigator:Aparecida Sadae Tanaka
Grantee:Tatiane Sanches Soares
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
Associated research grant:05/03514-9 - Studies of the physiological function and biotechnological potential of protease inhibitors and anti-hemostatics in hematophagous arthropods, AP.TEM


Aedes aegypti is one of the high world distribution mosquitoes and has medical importance because it is Dengue and Yellow fever vector. The increased number of disease cases transmitted by mosquitoes is due to the large number of larvae procreation sites and to the increase of mosquito's resistance to commercial insecticides. Protease inhibitors interfere in mosquito larvae digestive process inducing to a delay on their growth and development as well as significant mortality rates. In order to contribute in the development of new strategies in mosquito larvae control, we will use our experience in protease inhibitors and phage display system to construct a mutant inhibitor library exposed on M13 phage particles, using as a template molecule, a thrombin inhibitor produced by the mosquito Aedes aegypti, AaTI (Aedes aegypti thrombin inhibitor). This library will be used in specific digestion enzymes inhibitors selection from Ae. aegypti. The selected inhibitors will be produced in the yeast P. pastoris, purified, characterized and used in larval toxicity tests. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOARES, TATIANE SANCHES; SOARES TORQUATO, RICARDO JOSE; GONZALEZ, YAMILE GONZALEZ; ALVES LEMOS, FRANCISCO JOSE; TANAKA, APARECIDA SADAE. Production of serine protease inhibitors by mutagenesis and their effects on the mortality of Aedes aegypti L. larvae. PARASITES & VECTORS, v. 8, . (09/05405-3, 09/06003-6, 05/03514-9, 12/03657-8)

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