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Relationship among biochemical composition of dental biofilm, immunological and environmental factors and caries incidence in preschool children with early childhood caries

Grant number: 08/09510-3
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): April 01, 2009
Effective date (End): June 30, 2011
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal researcher:Marines Nobre dos Santos Uchôa
Grantee:Thaís Parisotto Ulmer
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


The prevalence of early childhood caries (ECC) in Brazil is high and its severity increases with age. The early loss of primary teeth can lead to serious problems in the development of the stomatognatic system, interfering in the children`s quality of life and also in the health conditions. Several clinical, biochemical, microbiological, immunological and environmental factors are able to influence the development and the progression of ECC lesions. This way, the aim of this project is to evaluate the relationship between the biochemical and microbiological composition of dental biofilm, immunological and environmental factors and early childhood caries incidence in one year follow up period. One hundred and thirty six children of both sexes, attending public nurseries and preschools in the city of Itatiba-SP will take part in the study. The children will be submitted to a clinical examination for the determination of the decayed, missing or filled-teeth (including initial lesions-white chalky spots), to dental biofilm collections for biochemical and microbiological analysis and to saliva collection for IgA quantification and further verification of the reaction between this antibody and the antigens of Streptococcus mutans (SM). In the biochemical analyses of the biofilm, calcium, fluoride, phosphorus and water-insoluble polysaccharide will be determined through the following methods, respectively: atomic absorption spectrophotometry, an ion-selective electrode and colorimetry. These variables will be expressed by dry biofilm weight (µg/mg). Quantitative microbiological culture techniques will be performed to determine the number of mutans streptococci (SM). The saliva samples will be evaluated by ELISA test in order to determine the levels of IgA. The reaction between IgA and Streptococcus mutans antigens will be evaluated by western blotting analysis. The environmental factors (diet, oral hygiene, family income, ethnicity, mother`s level of education) will be obtained by a diet chart and by a questionnaire. The results of caries incidence in children with ECC after one year follow up period will be expressed by means and standard deviations. The other data will be statistically analyzed through chi-square/Fischer`s exact test (±=0,05), followed by multiple logistic regression analysis with a 95% confidence interval. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
RIZZARDI, KARINA FERREIRA; AZEVEDO RODRIGUES, LIDIANY KARLA; STEINER-OLIVEIRA, CAROLINA; NOBRE-DOS-SANTOS, MARINES; PARISOTTO, THAIS MANZANO. Plaque Fluoride Levels as a Predictor of Caries Development in Early Childhood with High Sugar Exposure - A Preliminary Study. CLINICAL COSMETIC AND INVESTIGATIONAL DENTISTRY, v. 12, p. 71-78, . (08/09510-3)
PARISOTTO, T. M.; KING, W. F.; DUQUE, C.; MATTOS-GRANER, R. O.; STEINER-OLIVEIRA, C.; NOBRE-DOS-SANTOS, M.; SMITH, D. J.. Immunological and Microbiologic Changes during Caries Development in Young Children. Caries Research, v. 45, n. 4, p. 377-385, . (08/09510-3)
PARISOTTO, T. M.; STIPP, R.; RODRIGUES, L. K. A.; MATTOS-GRANER, R. O.; COSTA, L. S.; NOBRE-DOS-SANTOS, M.. Can insoluble polysaccharide concentration in dental plaque, sugar exposure and cariogenic microorganisms predict early childhood caries? A follow-up study. ARCHIVES OF ORAL BIOLOGY, v. 60, n. 8, p. 1091-1097, . (08/09510-3)

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