The present project aims to study scalable processes for the production of cationic liposomes to genic vaccination against tuberculosis. The scalable processes studied are top-down and bottom-up types. The first is based on the reduction of size of pre-formed liposomes, to the nanometric scale, through the application of impact and shear forces. Otherwise, the bottom-up approach uses the principles of microfluidics, associated to the interaction and diffusion in the interface of immiscible liquids, in order to form liposoes from the organization of molecules in the desirable size and polidispersity. The iposomes will be composed by the functional lipids: egg phosphatidyl choline (EPC),1,2-dioleoyl-sn-glicero-3-fosfoethanolamine (DOPE) e 1,2-dioleoyl-3-trimetilamonium-propane (DOTAP). Ethanol will be thesolent used. Previous studies of our group showed that that liposomes prepared in laboratorial scale by the dehidration-hidration method, when complexing DNAhsp65 on their surface, presented profilatic efficiency in mouse, when administered by intranasal route in a single dose, with a lower dosage, (25 µg ), compared to naked DNA or the same liposomes administered by intramuscular route. These results generated the submission of 01 patent and 01 addition certificate, 03 scientific articles and the approval of a PIPE FAPESP project, fase II (process n. 2007/01396-4) to the study of the scaling up of cationic functional liposomes to tuberculosis vaccination, to which this project is vinculated. The results obtained contribute to the development of processes for production of nanometric particles and to the production of the genic vaccine DNAhsp65 against tuberculosis.
News published in Agência FAPESP Newsletter about the scholarship: