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Effects of simulated pulpal pressure and immediate dentin sealing on the bond strength and nanoleakage patterns of resin-dentin interfaces produced by resin cements

Grant number: 08/07628-7
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2009
Effective date (End): February 28, 2011
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:André Figueiredo Reis
Grantee:Veronica Batista Santana
Host Institution: Universidade de Guarulhos (UNG). Campus Guarulhos-Centro. Guarulhos , SP, Brazil


The aim of this in vitro study is to evaluate the effects of intrapulpal pressure and immediate dentin sealing on the bond strength and nanoleakage patterns of resin-dentin interfaces produced by different resin cements. Two self-adhesive cements will be used: RelyX U-cem (3M Espe) and SmartCem (Dentsply Caulk); and two conventional resin cements: Rely X ARC (3M Espe), Panavia F (Kuraray). Eighty human third molars will be used. Teeth will be randomly distributed in 4 groups according to the luting materials and then will be assigned to 4 subgroups, according to the presence or not of positive pulpal pressure and the application or not of the immediate dentin sealing. Discs of pre-polymerized composite resin will be cemented according to manufacturers' instructions. After the storage periods, the restored teeth (n=5) will be serially sectioned, producing beams with a cross-sectional area of 0.8 mm2 at the resin-dentin interface, which will be tested in tension in a universal testing machine. Teeth luted with each material will be submitted to a nanoleakage protocol with AgNO3 after each storage period and will be prepared for observation in a Scanning and Transmission Electron Microscope. The bond strength and nanoleakage data will be statistically analyzed by two-way ANOVA and Tukey test.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FERREIRA-FILHO, R. C.; ELY, C.; AMARAL, R. C.; RODRIGUES, J. A.; ROULET, J-F; CASSONI, A.; REIS, A. F.. Effect of Different Adhesive Systems Used for Immediate Dentin Sealing on Bond Strength of a Self-Adhesive Resin Cement to Dentin. Operative Dentistry, v. 43, n. 4, p. 391-397, . (08/07628-7, 09/16261-2, 14/23401-3, 08/05179-0, 07/06083-4)
SANTANA, V. B.; DE ALEXANDRE, R. S.; RODRIGUES, J. A.; ELY, C.; REIS, A. F.. Effects of Immediate Dentin Sealing and Pulpal Pressure on Resin Cement Bond Strength and Nanoleakage. Operative Dentistry, v. 41, n. 2, p. 189-199, . (08/07628-7, 09/16261-2, 14/23401-3, 08/05179-0, 07/06083-4)

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