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In vivo evaluation of the activity of fluoxetine and desipramine in host response in periodontal disease

Grant number: 08/00566-6
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): August 01, 2008
Effective date (End): January 31, 2011
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal researcher:Gilson Cesar Nobre Franco
Grantee:Luciana Salles Branco de Almeida
Home Institution: Faculdade de Odontologia de Piracicaba (FOP). Universidade Estadual de Campinas (UNICAMP). Piracicaba , SP, Brazil


Periodontal disease (PD) is an infectious disease with a multifatorial etiology. Although bacterial dental biofilm is considered the main etiological factor, the destructive nature of PD is a result of the activation of the immune and inflammatory host responses. The antidepressant drugs fluoxetine and desipramine have shown, in recent studies, modulatory properties in osteoclast differentiation and antiinflammatory activity. However, the effects of such drugs on the host immune-inflammatory response during PD is still unknown. Thus, the aim of this study is to analyze the in vivo effects of fluoxetine and desipramine on the host's main immune and inflammatory reactions related to PD. Ninety rats (Wistar, Specific Pathogen Free) will be submitted to ligature-induced periodontitis in the first left lower molar. The animals will be treated once a day, by gastric intubation, with placebo, fluoxetine (20 mg/kd/day) or desipramine (20 mg/Kg/day) for 28 or 3 days, according to the methodology used. The animals treated for 28 days (n= 20 animals/group) will be sacrificed and the specimens separated for bone resorption measurement and histological analysis of inflammatory cells. The animals treated for 3 days (n= 10 animals/group) will be submitted to the following assays using the gingival tissues from mandíbula: mRNA levels of TNF-±, IL-1², COX-2, iNOs e MMP-9 assessed by RT-PCR; evaluation of protein production of TNF-± and IL-1² by immuno-enzimatic assay (ELISA); and MMP-9 activity detected by zimogram using SDS-PAGE. The authors of the present study believe that new mechanisms of action and effects of fluoxetine and desipramine on periodontal disease can be elucidated, serving as the basis for further studies aimed at developing an alternative adjuvant approach to treat PD. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRANCO-DE-ALMEIDA, LUCIANA S.; FRANCO, GILSON C.; CASTRO, MYRELLA L.; DOS SANTOS, JULIANA G.; ANBINDER, ANA LIA; CORTELLI, SHEILA C.; KAJIYA, MIKIHITO; KAWAI, TOSHIHISA; ROSALEN, PEDRO L.. Fluoxetine Inhibits Inflammatory Response and Bone Loss in a Rat Model of Ligature-Induced Periodontitis. Journal of Periodontology, v. 83, n. 5, p. 664-671, . (08/00566-6)
BRANCO-DE-ALMEIDA, LUCIANA S.; FRANCO, GILSON C. N.; CASTRO, MYRELLA L.; VIEIRA, MAYANA S.; GALVAO-MOREIRA, V, LEONARDO; CORTELLI, SHEILA C.; ANBINDER, ANA L.; KAWAI, TOSHIHISA; ROSALEN, PEDRO L.. Protective effects of desipramine on alveolar bone in experimental periodontitis. Journal of Periodontology, v. 91, n. 12, . (08/00566-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ALMEIDA, Luciana Salles Branco de. Effects of fluoxetine on immunoinflammatory responses associated with periodontal disease. 2011. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Odontologia de Piracicaba Piracicaba, SP.

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