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Evaluation of diazotrophic plant growth promoting bacteria in tropical grasses


It is estimated that in Brazil there are about 180 million hectares of pastures, with 60% with some degree of degradation. Recovery of degraded areas requires restoration of soil fertility, for plant growth and forage production, and the use of microbial inoculants with plant growth promoting bacteria may help in these processes. Due to the search for more viable and economical forms of nitrogen supply for forage grasses, inoculation with associative endophytic diazotrophic bacteria is a potential alternative because it contributes positively to plant nutrition, reducing the use of nitrogen fertilizers and thus benefiting the adoption of this technology in the agricultural environment. With the objective of evaluating the nutrition, development, bromatological composition and production of Panicum maximum cv. BRS Zuri submitted to inoculation with associative diazotrophic bacteria in Argisol. The first experiment will be conducted in a greenhouse, and the second in field conditions in Araçatuba-SP. The composition of the AB-V5 and AB-V6 strains of Azospirillum brasilense, Pseudomonas fluorescens CNPSo 2719 and the composition of AB-V6 and Rhizobium tropici CIAT 899, control (without nitrogen N) and without inoculation), with complete fertilization (N at a dose of 100 mg dm-3) and without inoculant, combination of N plus inoculation with strains and the combination of N plus inoculation associated with re-inoculation at shoot each cut. Each plot will consist of a pot of 8 dm3, totaling 55 plots. The variables to be analyzed will be plant height, number of leaves and tillers, dry mass of leaves and tillers, chlorophyll content, leaf / stem ratio, shoot / root ratio, dry mass of roots, macro and micronutrient levels, and the levels of PB, NDF, FDA and IVDMD. The second experiment under field conditions will be with the randomized blocks design, consisting of eleven treatments described above with four replicates. In addition to the variables to be analyzed above at the end of the experiment, an evaluation of the root system in two depths (0 to 10 cm and 10 to 20 cm) will be carried out, in which the root biomass will be determined, and the soil will be analyzed. soil chemical attributes (macro and micronutrients). The multidisciplinary work should allow to observe that the efficiency of forage production with high nutritional quality and a good pasture management is fundamental to guarantee the animal production, as well as pasture persistence and the mitigation of greenhouse gases. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SA, GEOVANA C. R.; HUNGRIA, MARIANGELA; MONTEIRO CARVALHO, CAROLINE LOPES; MOREIRA, ADONIS; NOGUEIRA, ARCO; HEINRICHS, REGES; SOARES FILHO, CECILIO VIEGA. Nutrients Uptake in Shoots and Biomass Yields and Roots and Nutritive Value of Zuri Guinea Grass Inoculated with Plant Growth-promoting Bacteria. Communications in Soil Science and Plant Analysis, v. 50, n. 22, p. 2927-2940, . (17/17573-4, 17/26364-0)
SA, G. C. R.; CARVALHO, C. L. M.; MOREIRA, A.; HUNGRIA, M.; NOGUEIRA, M. A.; HEINRICHS, R.; SOARES FILHO, V, C.. Biomass Yield, Nitrogen Accumulation and Nutritive Value of Mavuno Grass Inoculated with Plant Growth-promoting Bacteria. Communications in Soil Science and Plant Analysis, v. 50, n. 15, . (17/17573-4, 17/26364-0)

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