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Evaluation of the efficiency of lactic acid bacteria and Saccharomyces cerevisiae in reducing the bioavailability of aflatoxin M1 in Minas frescal cheese

Abstract

The project aims to verify the occurrence of aflatoxin M1 in milk samples and 'Minas frescal' cheese from dairies (monitored by Brazilian agriculture ministry), which yield this dairy product with and without the addition of lactic acid bacteria. Additionally, the specific aim of the proposed study will be to evaluate the efficiency of lactic acid bacteria and Saccharomyces cerevisiae for use as food additive to reduce the bioavailability of aflatoxin M1 in Minas frescal cheese. Fresh Minas cheeses will be experimentally produced with aflatoxin M1 contaminated milk, and lactic acid bacteria and Saccharomyces cerevisiae will be added during the cheese production process for testing the bioavailability reduction. The efficiency of these microorganisms in reducing the bioavailability of aflatoxin M1 in Minas frescal cheese will be evaluated through the high-performance liquid chromatography (HPLC) and the possible effects on the shelf life will be evaluated through physical-chemical and microbiological analyses. The identification of the binding mechanism between lactic acid bacteria, Saccharomyces cerevisiae and aflatoxin M1 will be performed by usage of the scanning electron microscopy. Statistical analyzes will be performed in accordance with the procedures established in the SAS General Linear Model to observe significant differences. It is expected with the present study to determine the effect of the addition of these microorganisms on the shelf life of Minas frescal cheese, and to identify the binding mechanism associated with lactic acid bacteria, Saccharomyces cerevisiae and aflatoxin M1. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BRUNA LEONEL GONÇALVES; ROMULO DUTRA ULIANA; SARAH HWA LEE; CAROLINA FERNANDA COPPA; CARLOS AUGUSTO FERNANDES DE OLIVEIRA; ELIANA SETSUKO KAMIMURA; CARLOS HUMBERTO CORASSIN. Use of scanning electron microscopy and high-performance liquid chromatography to assess the ability of microorganisms to bind aflatoxin M1 in Minas Frescal cheese. FOOD SCIENCE AND TECHNOLOGY, n. ahead, p. -, . (17/19683-1, 17/20081-6)
CAMPAGNOLLO, FERNANDA B.; KHANEGHAH, AMIN MOUSAVI; BORGES, LILIANA L.; BONATO, MELINA A.; FAKHRI, YADOLAH; BARBALHO, CAIO B.; BARBALHO, RICARDO L. C.; CORASSIN, CARLOS H.; OLIVEIRA, CARLOS A. F.. In vitro and in vivo capacity of yeast-based products to bind to aflatoxins B-1 and M-1 in media and foodstuffs: A systematic review and meta-analysis. Food Research International, v. 137, . (17/20081-6)
BRUNA LEONEL GONÇALVES; ROMULO DUTRA ULIANA; CAROLINA FERNANDA SENGLING CEBIN COPPA; SARAH HWA IN LEE; ELIANA SETSUKO KAMIMURA; CARLOS AUGUSTO FERNANDES OLIVEIRA; CARLOS HUMBERTO CORASSIN. Aflatoxin M1: biological decontamination methods in milk and cheese. FOOD SCIENCE AND TECHNOLOGY, v. 42, . (17/19683-1, 17/20081-6)
MOLLER, CLEIDE OLIVEIRA DE ALMEIDA; FREIRE, LUISA; ROSIM, ROICE ELIANA; MARGALHO, LARISSA PEREIRA; BALTHAZAR, CELSO FASURA; FRANCO, LARISSA TUANNY; SANT'ANA, ANDERSON DE SOUZA; CORASSIN, CARLOS HUMBERTO; RATTRAY, FERGAL PATRICK; OLIVEIRA, CARLOS AUGUSTO FERNANDES DE. Effect of Lactic Acid Bacteria Strains on the Growth and Aflatoxin Production Potential of Aspergillus parasiticus, and Their Ability to Bind Aflatoxin B-1, Ochratoxin A, and Zearalenone in vitro. FRONTIERS IN MICROBIOLOGY, v. 12, . (17/20081-6, 17/03899-5, 15/25641-4, 16/21041-5, 18/24540-8)
GONCALVES, BRUNA LEONEL; MUAZ, KHURRAM; SENGLING CEBIN COPPA, CAROLINA FERNANDA; ROSIM, ROICE ELIANA; KAMIMURA, ELIANA SETSUKO; FERNANDES OLIVEIRA, CARLOS AUGUSTO; CORASSIN, CARLOS HUMBERTO. Aflatoxin M-1 absorption by non-viable cells of lactic acid bacteria and Saccharomyces cerevisiae strains in Frescal cheese. Food Research International, v. 136, . (17/20081-6, 17/19683-1)
GONCALVES, BRUNA L.; ULLIANA, ROMULO D.; RAMOS, GUSTAVO L. P. A.; CRUZ, ADRIANO G.; OLIVEIRA, CARLOS A. F.; KAMIMURA, ELIANA S.; CORASSIN, CARLOS H.. Occurrence of aflatoxin M-1 in milk and Minas Frescal cheese manufactured in Brazilian dairy plants. INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY, v. 74, n. 2, . (17/19683-1, 17/20081-6)
BRUNA LEONEL GONÇALVES; ROMULO DUTRA ULIANA; SARAH HWA LEE; CAROLINA FERNANDA COPPA; CARLOS AUGUSTO FERNANDES DE OLIVEIRA; ELIANA SETSUKO KAMIMURA; CARLOS HUMBERTO CORASSIN. Use of scanning electron microscopy and high-performance liquid chromatography to assess the ability of microorganisms to bind aflatoxin M1 in Minas Frescal cheese. FOOD SCIENCE AND TECHNOLOGY, v. 42, . (17/20081-6, 17/19683-1)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.