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Insect digestion: a molecular, cellular, physiological and evolutionary approach

Abstract

The objective of this project is to describe the spatial organization of the midgut physiological processes and their underlying molecular events of insect types pertaining to different orders. The aim of these studies is to find out general principles from which hypotheses can be advanced in relation to the digestive and absorptive processes and tissue metabolic interactions of insects not previously studied. Such studies are necessary for developing new insect control procedures. The project is divided into 3 parts: (a) Midgut molecular physiology and tissue metabolic interactions, which models will be based on cDNA deep sequencing (obtained by Illumina) associated with data on digestive enzymes, buffering systems, and transporters, etc. The models will be tested by enzyme and transporter inhibitors and by protein silencing with the aid of RNAi; (b) Insect-purified or recombinant digestive enzymes in a search for peculiarities that set them apart from vertebrate enzymes and that led insects to overcome plant defenses; (c) Evolution of insect digestive systems. The present proposal will be improved by studying insect groups not considered before, such as leafhoppers, thrips, and cerambycid beetles, basal and more derived hymenopterans like wasps and ants. Another approach for evolution will be "phylotranscriptomics", the comparative analysis of whole or part of the midgut transcriptomes (obtained by Illumina sequencing) we have available or intend to prepare and phylogenomics of chosen insect genomes. (AU)

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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (13)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TERRA, WALTER R.; DIAS, RENATA O.; FERREIRA, CLELIA. Recruited lysosomal enzymes as major digestive enzymes in insects. BIOCHEMICAL SOCIETY TRANSACTIONS, v. 47, n. 2, p. 615-623, . (17/08103-4)
BARROSO, IGNACIO G.; CARDOSO, CHRISTIANE; FERREIRA, CLELIA; TERRA, WALTER R.. Transcriptomic and proteomic analysis of the underlying mechanisms of digestion of triacylglycerols and phosphatides and absorption and fate of fatty acids along the midgut of Musca domestica. Comparative Biochemistry and Physiology D-Genomics & Proteomics, v. 39, . (17/08103-4)
SILVA, CARLOS P.; DIAS, RENATA O.; BERNARDES, VANESSA; BARROSO, IGNACIO G.; CARDOSO, CHRISTIANE; FERREIRA, CLELIA; TERRA, WALTER R.. ecruitment of lysosomal cathepsins B, L and D as digestive enzymes in Coleopter. Insect Molecular Biology, v. 31, n. 2, . (17/08103-4)
BARROSO, IGNACIO G.; FUZITA, FELIPE J.; FERREIRA, CLELIA; TERRA, WALTER R.. Midgut fluxes and digestive enzyme recycling in Musca domestica: A molecular approach. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 241, . (17/08103-4)
TERRA, WALTER R.; FERREIRA, CLELIA. Evolutionary trends of digestion and absorption in the major insect orders. ARTHROPOD STRUCTURE & DEVELOPMENT, v. 56, . (17/08103-4)
FUZITA, FELIPE J.; PIMENTA, DANIEL C.; PALMISANO, GIUSEPPE; TERRA, WALTER R.; FERREIRA, CLELIA. Detergent-resistant domains in Spodoptera frugiperda midgut microvillar membranes and their relation to microapocrine secretion. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 235, p. 8-18, . (17/08103-4, 14/14183-2, 14/06863-3)
TERRA, WALTER R.; DIAS, RENATA O.; OLIVEIRA, PEDRO L.; FERREIRA, CLELIA; VENANCIO, THIAGO M.. Transcriptomic analyses uncover emerging roles of mucins, lysosome/secretory addressing and detoxification pathways in insect midguts. CURRENT OPINION IN INSECT SCIENCE, v. 29, p. 34-40, . (17/08103-4)
LIMA, LUCAS R.; DIAS, RENATA O.; FUZITA, FELIPE JUN; FERREIRA, CLELIA; TERRA, WALTER R.; SILVA-FILHO, MARCIO C.. The Evolution, Gene Expression Profile, and Secretion of Digestive Peptidases in Lepidoptera Species. CATALYSTS, v. 10, n. 2, . (14/50275-9, 17/08103-4)
BARROSO, IGNACIO G.; SANTOS, CARLA S.; BERTOTTI, MAURO; FERREIRA, CLELIA; TERRA, WALTER R.. Molecular mechanisms associated with acidification and alkalization along the larval midgut of Musca domestica. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, v. 237, . (15/20776-9, 17/08103-4)
PIMENTEL, ANDRE C.; BARROSO, IGNACIO G.; FERREIRA, JESSICA M. J.; DIAS, RENATA O.; FERREIRA, CLELIA; TERRA, WALTER R.. Molecular machinery of starch digestion and glucose absorption along the midgut of Musca domestica. JOURNAL OF INSECT PHYSIOLOGY, v. 109, p. 11-20, . (17/08103-4)
DIAS, RENATA O.; CARDOSO, CHRISTIANE; LEAL, CAMILA S.; RIBEIRO, ALBERTO F.; FERREIRA, CLELIA; TERRA, WALTER R.. Domain structure and expression along the midgut and carcass of peritrophins and cuticle proteins analogous to peritrophins in insects with and without peritrophic membrane. JOURNAL OF INSECT PHYSIOLOGY, v. 114, p. 1-9, . (17/08103-4)
FUZITA, FELIPE J.; PALMISANO, GIUSEPPE; PIMENTA, DANIEL C.; TERRA, WALTER R.; FERREIRA, CLELIA. A proteomic approach to identify digestive enzymes, their exocytic and microapocrine secretory routes and their compartmentalization in the midgut of Spodoptera frugiperda. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, v. 257, . (14/06863-3, 17/08103-4, 14/14183-2)
PIMENTEL, ANDRE C.; DIAS, RENATA O.; BIFANO, THAIS D.; GENTA, FERNANDO A.; FERREIRA, CLELIA; TERRA, WALTER R.. Cathepsins L and B in Dysdercus peruvianus, Rhodnius prolixus, and Mahanarva fimbriolata. Looking for enzyme adaptations to digestion. Insect Biochemistry and Molecular Biology, v. 127, . (17/08103-4)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.