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Study of mitochondrial translation factors

Grant number: 17/23921-5
Support Opportunities:Regular Research Grants
Duration: February 01, 2018 - May 31, 2020
Field of knowledge:Biological Sciences - Biochemistry - Metabolism and Bioenergetics
Principal Investigator:Mario Henrique de Barros
Grantee:Mario Henrique de Barros
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Our laboratory studies the mitochondrial biogenesis in Saccharomyces cerevisiae. In the last years we have characterized new factors involved in mitochondrial translation such as bL34, Msc6p and Aep3p. Here our first aim is to discriminate Msc6p function, with the identification of genetic interactors, proteins that interact with Msc6p as well as to identify the putative associated RNAs. In parallel we have studied the assembly of the large subunit of mitoribosome through conditional alleles obtained from bL34-encoded gene. Now we would like to continue these studies evaluating the importance of protein extensions present in mitoribosomes proteins but absent in their counterpart homologous in bacteria. Protein extensions present in 21 genes necessary for the biogenesis of the large (54S) subunit of the mitoribosome will be study. Finally we propose to modulate the mitochondrial magnesium content by genetic changes and its consequences in the mitoribosome biogenesis. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GUEDES-MONTEIRO, RAQUEL F.; FRANCO, LETICIA V. R.; MODA, BRUNO S.; TZAGOLOFF, ALEXANDER; BARROS, MARIO H.. 5 ` processing of Saccharomyces cerevisiae mitochondrial tRNAs requires expression of multiple genes. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1866, n. 5, p. 806-818, . (13/09482-8, 17/23921-5, 13/07937-8)
RIBEIRO FRANCO, LETICIA VELOSO; MODA, BRUNO S.; SOARES, MARIA A. K. M.; BARROS, MARIO H.. Msc6p is required for mitochondrial translation initiation in the absence of formylated Met-tRNA(fMet). FEBS Journal, v. 286, n. 7, p. 1407-1419, . (13/09482-8, 17/23921-5, 13/07937-8, 16/25907-7)
SANTOS, BARBARA; ZENG, RUI; JORGE, SASA F.; FERREIRA-JUNIOR, JOSE RIBAMAR; BARRIENTOS, ANTONI; BARROS, MARIO H.. Functional analyses of mitoribosome 54S subunit devoid of mitochondria-specific protein sequences. YEAST, . (13/07937-8, 20/05812-7, 18/03253-0, 17/23921-5)

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