Determination of ADAM10 and ADAM17 metalloproteinase activity on extracellular vesicles

Abstract

Visite Plan: 1. Seminar at LNBio on the 'Role of ectodomain shedding of signaling proteins on extracellular vesicle for the tumor-supporting communication between cancer and supporter cells - options for targeted cancer therapy'. 2. Laboratory work: Introduction and help in establishing a test system to determine the metalloproteinase activity on the surface of extracellular vesicles. The test is running in my lab in Cologne/Germany and I will help to establish this method at LNBio in Campinas. For that purpose, I will bring reagents, in particular selective, novel fluorescent substrates and help testing and evaluating the various samples from LNBio. 3. Meetings with other groups in SP (UNICAMP, USP, UNIFESP) to intensify existing collaborations and create a new collaborative research initiative on the 'Role of cancer cell EVs in tolerance induction within the tumor microenvironment'. We plan to create structures for a longlasting stable scientific cooperation, including student exchange between Cologne/Germany and São Paulo/BR. (AU)