BIOLOGICAL STUDY OF CARBON NANOTUBE, CHITOSAN AND HYDROXYAPATITE COMPOSITE WITH AND WITHOUT MESENCHYMAL STEM CELLS INTERACTION IN BONE TISSUE: RADIOGRAPHIC, BIOMECHANICAL AND HISTOLOGICAL EVALUATION.

Abstract

Nowadays some bone substitutes have been developed to help bone regeneration in case of tissue loss. To evaluate biocompatibility of a material in vivo implantation is necessary. The proposition of this research is the study of bone regenerations after a carbon nanotube, chitosan and hidrixyapatite (CNCH) composite implantation, with and without ovine mesenchymal stem cells (OMSC), in 50 rats and 18 sheep. Rats will be separated in groups: subcutaneous implantation in 20 rats (euthanasia after 7 and 30 days); calvarias implantation (euthanasia after 20 and 60 days). Samples will be evaluated by radiography, ultrasonography and thermography, previous and during and immediately after surgical procedures and before euthanasia. Histological evaluation will be done after euthanasia. Sheep will be submitted to tibial diaphisary ostectomy and osteosynthesis with locking compression plates. Six sheep will have the gap filled with CNCH, six with CNCH and OMSC and the other six won't have any filling in the bone gap. Previous and after surgery sheep will be clinical and image evaluated (radiography, densitometry, ultrasonography and Power Doppler), up to 90 days after surgery. After this period sheep will be submitted to euthanasia for biomechanical and histological evaluation of the tibias (light microscopy). The objective of this study is to investigate bone regeneration by image, biomechanical and hystological evaluations, after CNCH composite implantation with or without OMSC implantation in animals. (AU)