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Antigen discovery and development of serological diagnostic methods and vaccine approaches against the Zika Virus (ZIKV)

Grant number: 16/20045-7
Support type:Research Projects - Thematic Grants
Duration: December 01, 2017 - November 30, 2022
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:Luis Carlos de Souza Ferreira
Grantee:Luis Carlos de Souza Ferreira
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Pesquisadores principais:
Edison Luiz Durigon
Associated grant(s):20/06409-1 - Evaluation of humoral immune response and inflammatory response in patients with confirmed diagnosis of COVID-19 at Hospital Sírio Libanês and correlation with disease severity, AP.R
20/05204-7 - Development of SARS-CoV-2 auto-assembly protein-based nanovaccines, AP.R
Associated scholarship(s):19/16975-7 - Clinical and serological follow-up of mothers and their children diagnosed with previous Zika Virus infection, BP.IC
18/24683-3 - Production of envelope protein domain III of Zika Virus in Escherichia coli: evaluation of the protein obtained from soluble fraction and inclusion bodies, BP.DD
18/20864-3 - Antigen discovery and development of serological diagnostic methods and vaccine approaches against the zika virus (ZIKV), BP.TT
+ associated scholarships 18/15280-2 - Antigen Discovery and development of serological diagnostic methods and vaccine approaches against the zika virus (ZIKV), BP.TT
18/14459-9 - Investigation of zika virus (ZIKV) antigens to develop serological tests and vaccine approaches, BP.PD
18/08199-4 - Nanovaccines: a novel experimental concept applied to the development of subunit vacines., BP.PD
18/10913-7 - Development of a new in vitro inhibition strategy for Zika Virus and Influenza Virus using CRISPR-Cas13 RNA-editing technique as an alternative treatment, BP.DD
18/05346-6 - Development of specific serological tests from recombinant antigens derived from ZIKV, BP.TT
18/05337-7 - Evaluation of sorological diagnosis teste (NS1-REC ELISA) for Zika vírus: Zika vírus prevalence in women in the city of São Paulo, BP.IC
17/24769-2 - Zika virus in postpartum women and newborns: seroepidemiology and molecular characterization, BP.DD - associated scholarships

Abstract

The present grant application for a thematic research project has the main goal to offer financial support to different studies related to the Zika Vírus (ZIKV), presently under development at the Virology (LV) and Vaccine Research Laboratory (LDV) at the University of São Paulo (USP). The work is centered in four main themes: I) generation and application of ZIKV recombinant proteins aiming the development of vaccines and specific serological tests; II) development of experimental in vitro and in vivo models and establishment of biomarkers for the detection and monitoring of the immune responses induced by ZIKV as well as protective immunity induced by vaccine formulations; III) development and testing of different vaccine formulations capable to prevent ZIKV infection, which will include test of different candidate antigens, adjuvants and inoculation routes; and (IV) development of serological tests specific for ZIKV based on different recombinant antigens. The proposed work will be carried out as collaboration with different research groups from USP and other institutions, such as Butantan Institute, Fiocruz and Institute Pasteur. The LV e LDV have established competence in the development of vaccines and serological tests against Dengue Virus (DENV) as well as other virus and pathogens. The proposed projects are supported by the experience of both groups as demonstrated by publications during the last few years. In addition, results already obtained by the groups, in the scope of the present application, have already resulted in publications, patent applications and scientific discoveries which are already under use by different institutions in the country and abroad. The expected results will bring important impacts both on the serology of ZIKV as well as in researches focused on the development of vaccines for human use. The results to be achieved during the project will have relevant academic contributions and will led to the technological developments at short and medium term run. (AU)

Articles published in Pesquisa FAPESP Magazine about the research grant:
Avanza el coronavirus en Brasil 
Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications (26)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
STAN, RAZVAN C.; FRANCOSO, KATIA S.; ALVES, RUBENS P. S.; FERREIRA, LUIS CARLOS S.; SOARES, IRENE S.; DE CAMARGO, MARISTELA M.. Febrile temperatures increase in vitro antibody affinity for malarial and dengue antigens. PLoS Neglected Tropical Diseases, v. 13, n. 4, . (11/51778-6, 12/13032-5, 14/17595-0, 16/20045-7)
OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; MENDES, ERICA A.; LADNER, JASON T.; ANDREATA-SANTOS, ROBERT; ARAUJO, DANIELLE B.; BOTOSSO, VIVIANE F.; PAOLA, NICHOLAS D.; NETO, DANIEL F. L.; CUNHA, MARIELTON P.; et al. Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System. Viruses-Basel, v. 10, n. 11, . (14/16333-1, 14/03911-7, 14/17766-9, 13/26942-2, 16/20045-7, 16/08727-5, 14/27228-4, 15/25643-7, 13/07467-1, 16/08204-2, 16/03605-9)
KANNO, ALEX ISSAMU; DE CERQUEIRA LEITE, LUCIANA CEZAR; PEREIRA, LENNON RAMOS; RODRIGUES DE JESUS, MONICA JOSIANE; ANDREATA-SANTOS, ROBERT; DOS SANTOS ALVES, RUBENS PRINCE; DURIGON, EDISON LUIZ; DE SOUZA FERREIRA, LUIS CARLOS; GONCALVES, VIVIANE MAIMONI. Optimization and scale-up production of Zika virus Delta NS1 in Escherichia coli: application of Response Surface Methodology. AMB EXPRESS, v. 10, n. 1, . (12/23124-4, 14/17595-0, 16/20045-7)
SLAVOV, SVETOSLAV NANEV; GUARAGNA MACHADO, RAFAEL RAHAL; FERREIRA, ALEXANDER RODRIGO; SOARES, CAMILA PEREIRA; ARAUJO, DANIELLE BASTOS; LEAL OLIVEIRA, DANIELLE BRUNA; COVAS, DIMAS TADEU; DURIGON, EDISON LUIZ; KASHIMA, SIMONE. Zika virus seroprevalence in blood donors from the Northeastern region of Sao Paulo State, Brazil, between 2015 and 2017. Journal of Infection, v. 80, n. 1, p. 111-115, . (17/23205-8, 16/20045-7, 16/15049-3, 16/08727-5, 14/50947-7)
DE FREITAS DUTRA, VALERIA; BONET-BUB, CAROLINA; YOKOYAMA, ANA PAULA H.; ACHKAR, RUTH; MACHADO, RAFAEL RAHAL GUARAGNA; ASSUNCAO, MURILO; CANDELARIA, GABRIELA; SOARES, CAMILA PEREIRA; FACHINI, ROBERTA MARIA; FONTAO-WENDEL, RITA; et al. Anti-A and SARS-CoV-2: an intriguing association. Vox Sanguinis, v. 116, n. 5, . (17/24769-2, 16/20045-7, 18/23680-0, 20/06409-1)
BATISTA DOS SANTOS, JULIANA DE MELO; SOARES, CAMILA PEREIRA; MONTEIRO, FERNANDA RODRIGUES; MELLO, RALYRIA; DO AMARAL, JONATAS BUSSADOR; AGUIAR, ANDRESSA SIMOES; SOLEDADE, MARIANA PEREIRA; SUCUPIRA, CAROLINA; DE PAULIS, MILENA; ANDRADE, JULIANA BANNWART; et al. In Nasal Mucosal Secretions, Distinct IFN and IgA Responses Are Found in Severe and Mild SARS-CoV-2 Infection. FRONTIERS IN IMMUNOLOGY, v. 12, . (20/06409-1, 19/14115-0, 16/20045-7, 18/23680-0, 12/15165-2)
SABINO, CAETANO P.; SELLERA, FABIO P.; SALES-MEDINA, DOUGLAS F.; GUARAGNA MACHADO, RAFAEL RAHAL; DURIGON, EDISON LUIZ; FREITAS-JUNIOR, LUCIO H.; RIBEIRO, MARTHA S.. UV-C (254 nm) lethal doses for SARS-CoV-2. Photodiagnosis and Photodynamic Therapy, v. 32, . (17/24769-2, 17/22406-0, 16/20045-7)
AGUILAR TICONA, JUAN P.; NERY, NIVISON; DOSS-GOLLIN, SIMON; GAMBRAH, CLAUDIA; LESSA, MILLANI; RASTELY-JUNIOR, VALMIR; MATOS, ADRIANA; FREITAS, BRUNO DE PAULA; BORJA, ANA; WUNDER, ELSIO A.; et al. Heterogeneous development of children with Congenital Zika Syndrome-associated microcephaly. PLoS One, v. 16, n. 9, . (16/20045-7)
MACHADO, RAFAEL R. G.; GLASER, TALITA; ARAUJO, DANIELLE B.; PETIZ, LYVIA LINTZMAIER; OLIVEIRA, DANIELLE B. L.; DURIGON, GIULIANA S.; LEAL, ALESSANDRA L.; PINHO, JOAO RENATO R.; FERREIRA, LUIS C. S.; ULRICH, HENNING; et al. Inhibition of Severe Acute Respiratory Syndrome Coronavirus 2 Replication by Hypertonic Saline Solution in Lung and Kidney Epithelial Cells. ACS PHARMACOLOGY & TRANSLATIONAL SCIENCE, v. 4, n. 5, p. 1514-1527, . (20/06409-1, 19/00195-2, 18/07366-4, 17/24769-2, 16/20045-7, 20/04680-0)
DORLASS, ERICK GUSTAVO; MONTEIRO, CAIRO OLIVEIRA; VIANA, AMANDA OLIVEIRA; SOARES, CAMILA PEREIRA; GUARAGNA MACHADO, RAFAEL RAHAL; THOMAZELLI, LUCIANO MATSUMIYA; ARAUJO, DANIELLE BASTOS; LEAL, FABYANO BRUNO; CANDIDO, ERIKA DONIZETTE; TELEZYNSKI, BRUNA LAROTONDA; et al. Lower cost alternatives for molecular diagnosis of COVID-19: conventional RT-PCR and SYBR Green-based RT-qPCR. Brazilian Journal of Microbiology, v. 51, n. 3, . (17/25123-9, 16/20045-7, 18/23680-0, 17/24769-2)
YOKOYAMA, ANA PAULA H.; WENDEL, SILVANO; BONET-BUB, CAROLINA; FACHINI, ROBERTA M.; DAMETTO, ANA PAULA F.; BLUMM, FERNANDO; DUTRA, VALERIA F.; CANDELARIA, GABRIELA T. P.; SAKASHITA, ARACI M.; MACHADO, RAFAEL RAHAL GUARAGNA; et al. COVID-19 convalescent plasma cohort study: Evaluation of the association between both donor and recipient neutralizing antibody titers and patient outcomes. Transfusion, v. 61, n. 8, . (20/06409-1, 16/20045-7)
ANDREATA-SANTOS, ROBERT; DOS SANTOS ALVES, RUBENS PRINCE; PEREIRA, SARA ARAUJO; PEREIRA, LENNON RAMOS; DE FREITAS, CARLA LONGO; PEREIRA, SAMUEL SANTOS; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; CASTRO-AMARANTE, MARIA FERNANDA; PINHO FAVARO, MARIANNA TEIXEIRA; MATHIAS-SANTOS, CAMILA; et al. Transcutaneous Administration of Dengue Vaccines. Viruses-Basel, v. 12, n. 5, . (16/05570-8, 12/50362-3, 16/20045-7, 14/17595-0, 16/23560-0, 15/02352-7)
WENDEL, SILVANO; FACHINI, ROBERTA; FONTAO-WENDEL, RITA C. L.; MELLO, RALYRIA; VELASQUEZ, CARLOS VINICIUS; GUARAGNA MACHADO, RAFAEL RAHAL; BRITO, MAYRA ALTOBELLI; AMARAL, MARCELO; SOARES, CAMILA PEREIRA; ACHKAR, RUTH; et al. urrogate test performance for SARS-CoV-2 neutralizing antibodies (nAbs) for convalescent plasma (CCP): How useful could they be. Transfusion, v. 61, n. 12, . (17/24769-2, 20/06409-1, 16/20045-7, 18/23680-0)
FELISMINO, EDUARDO S.; SANTOS, JULIANA M. B.; ROSSI, MARCELO; SANTOS, CARLOS A. F.; DURIGON, EDISON L.; OLIVEIRA, DANIELLE B. L.; THOMAZELLI, LUCIANO M.; MONTEIRO, FERNANDA R.; SPERANDIO, ADRIANE; APOSTOLICO, JULIANA S.; et al. Better Response to Influenza Virus Vaccination in Physically Trained Older Adults Is Associated With Reductions of Cytomegalovirus-Specific Immunoglobulins as Well as Improvements in the Inflammatory and CD8(+) T-Cell Profiles. FRONTIERS IN IMMUNOLOGY, v. 12, . (20/06409-1, 16/20045-7, 12/15165-2, 19/14115-0)
WENDEL, SILVANO; FONTAO-WENDEL, RITA; FACHINI, ROBERTA; CANDELARIA, GABRIELA; SCURACCHIO, PATRICIA; ACHKAR, RUTH; BRITO, MAYRA; REIS, LUIZ FERNANDO; CAMARGO, ANAMARIA; AMANO, MARIANE; et al. A longitudinal study of convalescent plasma (CCP) donors and correlation of ABO group, initial neutralizing antibodies (nAb), and body mass index (BMI) with nAb and anti-nucleocapsid (NP) SARS-CoV-2 antibody kinetics: Proposals for better quality of CCP collections. Transfusion, v. 61, n. 5, . (17/24769-2, 16/20045-7, 18/23680-0, 20/06409-1)
DANIELLE BASTOS ARAUJO; RAFAEL RAHAL GUARAGNA MACHADO; DEYVID EMANUEL AMGARTEN; FERNANDA DE MELLO MALTA; GABRIEL GUARANY DE ARAUJO; CAIRO OLIVEIRA MONTEIRO; ERIKA DONIZETTI CANDIDO; CAMILA PEREIRA SOARES; FERNANDO GATTI DE MENEZES; ANA CAROLINA CORNACHIONI PIRES; et al. SARS-CoV-2 isolation from the first reported patients in Brazil and establishment of a coordinated task network. Memórias do Instituto Oswaldo Cruz, v. 115, . (20/06409-1, 20/04680-0, 17/25123-9, 17/24769-2, 18/21076-9, 16/20045-7, 18/23680-0)
PINHO FAVARO, MARIANNA TEIXEIRA; RODRIGUES-JESUS, MONICA JOSIANE; VENCESLAU-CARVALHO, ALEXIA ADRIANNE; DOS SANTOS ALVES, RUBENS PRINCE; PEREIRA, LENNON RAMOS; PEREIRA, SAMUEL SANTOS; ANDREATA-SANTOS, ROBERT; DE SOUZA FERREIRA, LUIS CARLOS. Nanovaccine based on self-assembling nonstructural protein 1 boosts antibody responses to Zika virus. Nanomedicine-Nanotechnology Biology and Medicine, v. 32, . (16/20045-7, 18/08199-4)
VENCESLAU-CARVALHO, ALEXIA ADRIANNE; FAVARO, MARIANNA TEIXEIRA DE PINHO; PEREIRA, LENNON RAMOS; RODRIGUES-JESUS, MONICA JOSIANE; PEREIRA, SAMUEL SANTOS; ANDREATA-SANTOS, ROBERT; ALVES, RUBENS PRINCE DOS SANTOS; CASTRO-AMARANTE, MARIA FERNANDA; RODRIGUES, KARINE BITENCOURT; DA SILVA, JAMILE RAMOS; et al. Nano-multilamellar lipid vesicles loaded with a recombinant form of the chikungunya virus E2 protein improve the induction of virus-neutralizing antibodies. Nanomedicine-Nanotechnology Biology and Medicine, v. 37, . (17/23281-6, 16/19145-7, 16/08204-2, 17/24769-2, 16/20045-7, 16/23560-0)
PEREIRA, SAMUEL SANTOS; ANDREATA-SANTOS, ROBERT; PEREIRA, LENNON RAMOS; SOARES, CAMILA PEREIRA; FELIX, ALVINA CLARA; JUNGMANN CARDOSO DE ANDRADE, PATRICIA DE MELLO; DURIGON, EDISON LUIS; ROMANO, CAMILA MALTA; DE SOUZA FERREIRA, LUIS CARLOS. NS1-based ELISA test efficiently detects dengue infections without cross-reactivity with Zika virus. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 112, p. 202-204, . (17/09661-0, 16/05570-8, 16/20045-7)
MENDRONE-JUNIOR, ALFREDO; DINARDO, CARLA LUANA; FERREIRA, SUZETE CLEUZA; NISHYA, ANNA; SALLES, NANCI ALVES; DE ALMEIDA NETO, CESAR; HAMASAKI, DEBORA TOSHEI; FACINCANI, TILA; DE OLIVEIRA ALVES, LUCAS BASSOLLI; MACHADO, RAFAEL RAHAL GUARAGNA; et al. Correlation between SARS-COV-2 antibody screening by immunoassay and neutralizing antibody testing. Transfusion, v. 61, n. 4, p. 1181-1190, . (16/20045-7, 20/06409-1, 17/24769-2)
RODRIGUES, JULIANA FALCAO; LOURENCO, ROGERIO FERREIRA; MAEDA, DENICAR LINA NASCIMENTO FABRIS; DE JESUS CINTRA, MARIANA; NAKAO, NAOMI; MATHIAS-SANTOS, CAMILA; LUIZ, WILSON BARROS; DE SOUZA FERREIRA, LUIS CARLOS. Strain-specific transcriptional and posttranscriptional regulation of heat-labile toxin expression by enterotoxigenic Escherichia coli. Brazilian Journal of Microbiology, v. 51, n. 2, . (16/20045-7)
ARAUJO, SERGIO C.; PEREIRA, LENNON R.; ALVES, RUBENS P. S.; ANDREATA-SANTOS, ROBERT; KANNO, ALEX I.; FERREIRA, LUIS CARLOS S.; GONCALVES, VIVIANE M.. Anti-Flavivirus Vaccines: Review of the Present Situation and Perspectives of Subunit Vaccines Produced in Escherichia coli. VACCINES, v. 8, n. 3, . (18/24683-3, 16/20045-7, 16/23560-0, 19/18205-4, 15/02352-7)
NERY, JR., NIVISON; AGUILAR TICONA, JUAN P.; GAMBRAH, CLAUDIA; DOSS-GOLLIN, SIMON; AROMOLARAN, ADEOLU; RASTELY-JUNIOR, VALMIR; LESSA, MILLANI; SACRAMENTO, GIELSON A.; CRUZ, JAQUELINE S.; DE OLIVEIRA, DAIANA; et al. Social determinants associated with Zika virus infection in pregnant women. PLoS Neglected Tropical Diseases, v. 15, n. 7, . (16/20045-7)
WENDEL, SILVANO; KUTNER, JOSE MAURO; MACHADO, RAFAEL; FONTAO-WENDEL, RITA; BUB, CAROLINA; FACHINI, ROBERTA; YOKOYAMA, ANA; CANDELARIA, GABRIELA; SAKASHITA, ARACI; ACHKAR, RUTH; et al. Screening forSARS-CoV-2 antibodies in convalescent plasma in Brazil: Preliminary lessons from a voluntary convalescent donor program. Transfusion, v. 60, n. 12, p. 2938-2951, . (17/24769-2, 16/20045-7, 18/23680-0, 20/06409-1)
ANDREATA-SANTOS, ROBERT; PEREIRA, SAMUEL SANTOS; PEREIRA, LENNON RAMOS; FELIX, ALVINA CLARA; ROMANO, CAMILA MALTA; DE SOUZA FERREIRA, LUIS CARLOS. Specificity of NS1-based immunochromatographic tests for dengue virus with regard to the Zika virus protein. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, v. 95, p. 276-278, . (16/23560-0, 16/20045-7)
MENDES, ERICA ARAUJO; BAIRROS DE PILGER, DENISE REGINA; DE SEIXAS SANTOS NASTRI, ANA CATHARINA; MALTA, FERNANDA DE MELLO; PASCOALINO, BRUNO DOS SANTOS; CARNEIRO D'ALBUQUERQUE, LUIZ AUGUSTO; BALAN, ANDREA; GONDIM DE FREITAS JR, LUCIO HOLANDA; DURIGON, EDISON LUIS; CARRILHO, FLAIR JOSE; et al. Sofosbuvir inhibits yellow fever virus in vitro and in patients with acute liver failure. ANNALS OF HEPATOLOGY, v. 18, n. 6, p. 816-824, . (16/20045-7)

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