Advanced search
Start date
Betweenand

Trypanosoma Cruzi parasitic load analysis in sequential endomyocardial biopsies of chagasic, heart-transplanted patients who presented reactivation of the disease

Abstract

Reactivation of Chagas' disease (RCD) after heart transplantation is common, although rarely fatal. When affecting the heart, the diagnosis is usually established by endomyocardial biopsy (EMB). However, the histological picture of RCD is similar to acute cellular rejection, with inflammatory infiltration of the myocardium and cardiomyocyte aggression. The differential diagnosis between both entities, which present radically different treatment, is based on the identification of T. cruzi parasites or its antigenic fragments in HE stained sections or immunohistochemistry (IHC), in RCD. However, due to tissue scarcity of EMB, parasites can be missed by histological methods in RCD cases and more sensitive methods of parasitic detection are desirable. In this project we propose to evaluate retrospectively the T. cruzi parasitic load (PL) through quantitative, real-time PCR in 147 EMB of 14 patients; 77 obtained before RCD, 14 which substantiate that diagnosis and 56 obtained after the diagnosis when patients were on specific, antiparasitic treatment. Curves relating PL and time will be obtained for every patient, in order to understand how PL evolves in the myocardium, culminating in RCD and how it evolves after treatment. We are particularly interested in the evolution of PL before the occurrence of RCD, because its analysis can permit an early diagnosis of the complication, before T. cruzi parasites can be identified by the traditional methods (HE and IHC). The precocious diagnosis of RCD would permit a more adequate and effective treatment of the patients. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BENVENUTI, LUIZ ALBERTO; ROGGERIO, ALESSANDRA; NISHIYA, ANNA SHOKO; MANGINI, SANDRIGO; LEVI, JOSE EDUARDO. Sequential measurement of Trypanosoma cruzi parasitic load in endomyocardial biopsies for early detection and follow-up of Chagas disease reactivation after heart transplantation. TRANSPLANT INFECTIOUS DISEASE, v. 22, n. 1, . (17/07946-8)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.