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Synthesis and functionalization of bimetallic nanoparticles for application in photodynamic therapy of cancer

Abstract

Despite the continuous development of Photodynamic Therapy of cancer, the consolidation of this therapy in clinical application is hold back due to the unsatisfactory tumor targeting and short circulation time of photosensitizers (FS). The coupling of FS to nanoparticles (NPs) has emerged as a useful tool to overcome these limitations. The NPs have enhanced penetration and retention in tumors due to their irregular vasculature and their larger porous compared to the normal tissues. Additionally, to the improved tumor targeting, NPs can enhance therapeutic efficiency of FS by protecting them from deactivation, avoiding FS aggregation and providing the control of FS photoactivity. As an example, metallic surfaces can enhance the efficiency of FS in generating reactive oxygen species, especially singlet oxygen, which is known to cause cellular death through apoptosis. Although this effect has been reported for monometallic NPs, the use of bimetallic NPs to control FS photoactivity was poorly explored until now. Since the optical and catalytic properties of bimetallic NPs has been shown to be superior than the properties of monometallic NPs, it is expected that bimetallic NPs will be more efficient in enhancing FS photoactivity. In this way, it is proposed in this project the synthesis of bimetallic NPs and their functionalization with a FS with the goal of obtaining photosensitizing NPs of high photoactivity. Besides that, it is expected that these NPs will present better tumor targeting not only by passive mechanism but also by active mechanism since the peptide C (CVNHPAFAC), which was previously identified as a tumor-homing peptide, will also be linked to the NPs surface. Another advantage of bimetallic NPs is their superior antimicrobial activity. For this reason, it is also expected that the NPs to be developed in this project will present antimicrobial activity, which can avoid infectious processes during treatment. Photosensitizing NPs will be developed based on the following bimetallic combinations: gold and silver, silver and iron and gold and platinum. Aiming at the improved antitumoral activity of these NPs, their surface will be additionally functionalized with the peptides YISCYNGATSYNQKFK, RASQSVSSYLA e GQYGNLWFAY. These peptides had their antitumoral and antimetastatic activities in vivo demonstrated in previous works. Following the morphological, photophysical and photochemical characterization, the photoactivity of the NPs developed here will be evaluated through in vitro assays with murine metastatic melanoma cells. (AU)

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Scientific publications (13)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA SILVA, DIEGO MORAIS; CANUTO DE MENEZES, BEATRIZ ROSSI; NONATO BEZZON, VINICIUS DANILO; DO AMARAL MONTANHEIRO, THAIS LARISSA; DE MACEDO, ERENILDA FERREIRA; TADA, DAYANE BATISTA; PETRACONI, GILBERTO; THIM, GILMAR PATROCINIO. Phase transition of TiO2 nanoparticles into titanate nanorods via hydrothermal reactions. SN APPLIED SCIENCES, v. 1, n. 8, . (17/24873-4, 19/00568-3, 18/12035-7, 17/02846-5, 17/01697-6)
DE MELO SILVA, ISABELLE SOUZA; DO AMORIM COSTA GASPAR, LIVIA MARIA; OLIVEIRA ROCHA, ADRIELY MARIA; DA COSTA, LUIZ PEREIRA; TADA, DAYANE BATISTA; FRANCESCHI, ELTON; PADILBA, FRANCINE FERREIRA. Encapsulation of Red Propolis in Polymer Nanoparticles for the Destruction of Pathogenic Biofilms. AAPS PHARMSCITECH, v. 21, n. 2, . (17/01697-6)
DE OLIVEIRA, THAIS DOLZANY; TRAVASSOS, LUIZ R.; ARRUDA, DENISE COSTA; TADA, DAYANE BATISTA. Intracellular Targeting of Poly Lactic-Co-Glycolic Acid Nanoparticles by Surface Functionalization with Peptides. JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v. 17, n. 7, p. 1320-1329, . (11/23895-8, 15/05980-9, 17/01697-6, 18/11972-7, 10/51423-0)
MAGALHAES, JESSICA A.; ARRUDA, DENISE C.; BAPTISTA, MAURICIO S.; TADA, DAYANE B.. Co-Encapsulation of Methylene Blue and PARP-Inhibitor into Poly(Lactic-Co-Glycolic Acid) Nanoparticles for Enhanced PDT of Cancer. NANOMATERIALS, v. 11, n. 6, . (18/22890-1, 17/01697-6)
HURTADO, CAROLINA RAMOS; WACHESK, CRISTIANE DA COSTA; QUEIROZ, RAFAELA CAMPOS; DE MACEDO, ERENILDA FERREIRA; BORJA DE OLIVEIRA CORREIA, REBECA FALCAO; TAIARIOL, THALITA SANI; DINIZ, MILTON FARIA; ISAIAS DOS SANTOS, ALEXANDRE MARTINS; DO AMARAL MONTANHEIRO, THAIS LARISSA; HURTADO, GABRIELA RAMOS; et al. A simple procedure to obtain nanodiamonds from leftover of HFCVD system for biological application. SN APPLIED SCIENCES, v. 2, n. 3, . (12/15857-1, 17/01697-6)
MAGALHAES, JESSICA A.; FERNANDES, ADJACI U.; JUNQUEIRA, HELENA C.; NUNES, BRUNA C.; CURSINO, THAIS A. F.; FORMAGGIO, DANIELA M. D.; BAPTISTA, MAURICIO S.; TADA, DAYANE B.. Bimetallic nanoparticles enhance photoactivity of conjugated photosensitizer. Nanotechnology, v. 31, n. 9, . (18/22890-1, 17/01697-6)
RODRIGUES, LINA D. A.; HURTADO, CAROLINA R.; MACEDO, ERENILDA F.; TADA, DAYANE B.; GUERRINI, LILIA M.; OLIVEIRA, MAURICIO P.. Colloidal properties and cytotoxicity of enzymatically hydrolyzed cationic starch-graft-poly(butyl acrylate-co-methyl methacrylate) latex by surfactant-free emulsion polymerization for paper coating application. Progress in Organic Coatings, v. 145, . (18/12469-7, 17/01697-6)
DUCATTI FORMAGGIO, DANIELA MARIA; DE OLIVEIRA NETO, XISTO ANTONIO; ALCANTARA RODRIGUES, LINA DAYSE; DE ANDRADE, VITOR MARTINS; NUNES, BRUNA C.; LOPES-FERREIRA, MONICA; FERREIRA, FABIANA G.; WACHESK, CRISTIANE C.; CAMARGO, EMERSON R.; CONCEICAO, KATIA; et al. In vivo toxicity and antimicrobial activity of AuPt bimetallic nanoparticles. JOURNAL OF NANOPARTICLE RESEARCH, v. 21, n. 11, . (11/23895-8, 17/01697-6)
HURTADO, CAROLINA RAMOS; HURTADO, GABRIELA RAMOS; DE CENA, GABRIELLE LUPETI; QUEIROZ, RAFAELA CAMPOS; SILVA, ALEXANDRE VIEIRA; DINIZ, MILTON FARIA; DOS SANTOS, VERONICA RIBEIRO; TRAVA-AIROLDI, VLADIMIR; BAPTISTA, MAURICIO DA SILVA; TSOLEKILE, NCEDIWE; et al. Diamond Nanoparticles-Porphyrin mTHPP Conjugate as Photosensitizing Platform: Cytotoxicity and Antibacterial Activity. NANOMATERIALS, v. 11, n. 6, . (19/09737-2, 19/20111-8, 17/01697-6)
OLIVEIRA, RODRIGO L. M. S.; BARBOSA, LUCAS; HURTADO, CAROLINA R.; RAMOS, LUCAS DE P.; MONTANHEIRO, THAIS L. A.; OLIVEIRA, LUCIANE D.; TADA, DAYANE B.; TRICHES, ELIANDRA DE SOUSA. Bioglass-based scaffolds coated with silver nanoparticles: Synthesis, processing and antimicrobial activity. Journal of Biomedical Materials Research Part A, v. 108, n. 12, . (17/01697-6, 15/24659-7)
BRAZUNA, LORENA PORTELA; TABUTI, THIAGO GALEOTE; SILVA, ADRIELLE DE PAULA; TADA, DAYANE BATISTA; POLITI, MARIO JOSE; BACANI, REBECA; TRIBONI, EDUARDO REZENDE. Effect of lithium and sodium ions on the size and morphology of ZnO nanoparticles synthesized by a glycerol-urea route. NEW JOURNAL OF CHEMISTRY, v. 43, n. 48, p. 18988-18995, . (17/13839-0, 13/08166-5, 17/01697-6)
TENORIO, FERNANDA SILVA; DO AMARAL MONTANHEIRO, THAIS LARISSA; ISAIAS DOS SANTOS, ALEXANDRE MARTINS; SILVA, MATEUS DOS SANTOS; LEMES, ANA PAULA; TADA, DAYANE BATISTA. Chitosan hydrogel covalently crosslinked by gold nanoparticle: Eliminating the use of toxic crosslinkers. Journal of Applied Polymer Science, v. 138, n. 6, . (17/01697-6, 17/24873-4, 13/27064-9)
DE MACEDO, ERENILDO FERREIRA; DUCATTI FORMAGGIO, DANIELA MARIA; SANTOS, NIVIA SALLES; TADA, DAYANE BATISTA. Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal. SENSORS, v. 17, n. 12, . (11/23895-8, 17/01697-6)

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