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Metabolomics signatures on the influence of holding time on increasing boar spermatozoa cryotolerance


Despite the great scientific investments, boar semen cryopreservation stills an important challenge for science, regarding post-thawed boar semen quality, regardless pre-cryopreservation quality or cryopreservation protocol used. Some proteins have been described as freezeability biological marks of boar semen. However, seminal plasma have a wide range components, so it lead us to believe that not only proteins but also lipids, ions and others components could be beneficial or detrimental to boar semen freezeability. Jointly, holding time (incubation time in own seminal plasma before cryopreservation) has been used to improve post-thawed boar semen quality. Holding time allows greater contact of spermatozoa with seminal plasma components, and it is reported to improve cryotolerance at low temperatures. It led us to postulate whether cryopreservation with or without holding time change the interaction between seminal plasma molecules and spermatozoa, and then freezeability. Thus, three experiments will be conducted in order to understand the influence of holding time on increasing boar spermatozoa cryotolerance. First experiment aims to determine the optimal holding time to boar semen cryopreservation. Second experiment aims to study the differences between boar spermatozoa freezeability and holding time. Finally, the third experiment aims to elucidated, through metabolomics, which seminal molecule contributes for the increasing in boar spermatozoa freezeability (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TORRES, MARIANA A.; MONTEIRO, MATHEUS S.; PASSARELLI, MARINA S.; PAPA, FREDERICO O.; DELL'AQUA, JR., JOSE ANTONIO; ALVARENGA, MARCO ANTONIO; MARTINS, SIMONE M. M. K.; DE ANDRADE, ANDRE F. C.. The ideal holding time for boar semen is 24 h at 17 degrees C prior to short-cryopreservation protocols. CRYOBIOLOGY, v. 86, p. 58-64, . (15/17620-7, 16/24690-4, 16/09441-8)
PASSARELLI, MARINA DA SILVA; PINOTI PAVANELI, ANA PAULA; RAVAGNANI, GISELE MOURO; MARTINS, MATHEUS PASINI; PEDROSA, ANA CAROLINA; MASSAMI KITAMURA MARTINS, SIMONE MARIA; DE ALCANTRA ROCHA, NATHALIA RAISSA; BITTAR RIGO, VICTOR HENRIQUE; YASUI, GEORGE; YESTE, MARC; et al. Effects of different equilibration times at 5 degrees C on boar sperm cryotolerance. Animal Reproduction Science, v. 219, . (16/24690-4, 19/01124-1, 17/10821-2)
PEDROSA, ANA CAROLINA; TORRES, MARIANA ANDRADE; ALKMIN, DIEGO VILELA; PINZON, JORGE E. P.; MASSAMI KITAMURA MARTINS, SIMONE MARIA; DA SILVEIRA, JULIANO COELHO; CESAR DE ANDRADE, ANDRE FURUGEN. Spermatozoa and seminal plasma small extracellular vesicles miRNAs as biomarkers of boar semen cryotolerance. Theriogenology, v. 174, p. 60-72, . (16/24690-4, 18/12500-1, 15/21829-9, 14/22887-0)
MONTEIRO, MATHEUS SALIBA; TORRES, MARIANA ANDRADE; PASSARELLI, MARINA DA SILVA; MARTINS, MATHEUS PASSINI; RAVAGNANI, GISELE MOURO; PAPA, FREDERICO OZANAM; ALVARENGA, MARCO ANTONIO; DELL'AQUA JUNIOR, JOSE ANTOMO; YASUI, GEORGE SHIGUEKI; MARTINS, SIMONE MARIA MASSAMI KITAMURA; et al. Impact of cryopreservation protocols (one- and two-step) on boar semen quality at 5 degrees C and post-thawing. Animal Reproduction Science, v. 247, p. 9-pg., . (17/16987-0, 16/24690-4, 17/10821-2)
TORRES, MARIANA A.; PEDROSA, ANA CAROLINA; NOVAIS, FRANCISCO JOSE; ALKMIN, V, DIEGO; COOPER, BRUCE R.; YASUI, GEORGE S.; FUKUMASU, HEIDGE; MACHATY, ZOLTAN; DE ANDRADE, ANDRE F. C.. etabolomic signature of spermatozoa established during holding time is responsible for differences in boar sperm freezabilit. BIOLOGY OF REPRODUCTION, v. 106, n. 1, p. 213-226, . (16/09441-8, 18/03924-2, 16/24690-4)

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