Advanced search
Start date
Betweenand

Natural proteopeptides from the Brazilian fauna, flora and microbiota as potential models for the rational development of new drugs of therapeutic use: isolation, structure elucidation, chemical synthesis and functional activity assays

Abstract

Nature offers us an impressive number of different types of biologically active peptides. In multicellular organisms there is a large number of different types of peptides with cell signaling functions, physiological regulation, immune defense, regulation of growth, homeostasis, reproduction, neurotoxicity, production of pain/analgesia, inflammation, among other functions this sense, animal poisons offer many examples of toxins from snakes, scorpions, spiders, insects and marine organisms. The natural peptide toxins inspired the development of drugs for the treatment of chronic pain. Plants have provided important structural models for developing new anti-cancer drugs, and the microorganisms have provided inspiration for the development of several lines of antibiotics. Thus, the objective of this study is to develop a bioprospective work with some specimens of venomous arthropod from Brazilian fauna, with some plants from the flora of Brazilian savanna, and with some microorganisms from Brazilian microbiota, aiming the identification of proteopeptide components presenting novel chemical structures, and interesting functional activities, that could be used as models for the rational development of new drugs for therapeutic use. For these purposes, the detection and isolation approaches will be performed using different types of liquid chromatography, high performance and LC-MS and MS/MS (or MSn) systems. The identified components will have their chemical structures assigned by using a variety of spectroscopic techniques such as spectroscopy, high resolution mass spectrometry, circular dichroism spectroscopy NMR analysis (technical two-dimensional total correlation spectroscopy) to determine the tertiary structure (for some peptides) and/or secondary structure (for other peptides), molecular modeling and molecular dynamics simulations. The peptides will be synthesized in solid phase (combining a series of different experimental strategies) using automated/robotic synthesis system. The Structural Biology Laboratory and Zooquímica the CEIS/IBRC-UNESP is fitted to perform these tasks, and has expertise to develop multiple processes of synthesis in solid phase for linear, cyclic peptides, bridged disulfide bonds (intra and/or extramolecular), and peptides having chemical modifications of the chains sides for different amino acid residues. Synthetic peptides (reproducing natural peptides) will then be purified and subjected to a series of biological activity assays (mast cell degranulation, chemotaxis, antibiosis, inhibition of biofilm formation, inhibition activity of the enzymes COX-1 and COX-2, and anti-mitogenic activity, and toxicity against insect and fish models). All of these bioassays will be conducted with the use of cell cultures, recombinant proteins, and alternative animal models; no mammal will be used. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (18)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE SOUZA, CAROLINE LACERRA; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ESTEVES, FRANCIELE GREGO; PEREZ-RIVEROL, AMILCAR; ROMANI FERNANDES, LUIS GUSTAVO; ZOLLNER, RICARDO DE LIMA; PALMA, MARIO SERGIO. Revisiting Polybia paulista wasp venom using shotgun proteomics - Insights into the N-linked glycosylated venom proteins. JOURNAL OF PROTEOMICS, v. 200, p. 60-73, . (17/04680-7, 13/26451-9, 17/22405-3, 16/16212-5, 17/10373-0)
COSTA-LEONARDO, ANA MARIA; DA SILVA, IAGO BUENO; JANEI, VANELIZE; ESTEVES, FRANCIELE GREGO; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; PALMA, MARIO SERGIO. Worker Defensive Behavior Associated with Toxins in the Neotropical Termite Neocapritermes braziliensis (Blattaria, Isoptera, Termitidae, Termitinae). Journal of Chemical Ecology, v. 45, n. 9, p. 755-767, . (16/16212-5, 13/26451-9, 17/10373-0)
SINGULANI, JUNYA DE LACORTE; OLIVEIRA, LARIANE TEODORO; RAMOS, MARINA DORISSE; FREGONEZI, NATHALIA FERREIRA; GOMES, PAULO CESAR; GALEANE, MARIANA CRISTINA; PALMA, MARIO SERGIO; FUSCO ALMEIDA, ANA MARISA; SOARES MENDES GIANNINI, MARIA JOSE. he Antimicrobial Peptide MK58911-NH2 Acts on Planktonic, Biofilm, and Intramacrophage Cells of Cryptococcus neoforman. Antimicrobial Agents and Chemotherapy, v. 65, n. 12, . (16/16212-5, 17/06658-9)
CASTILHO DE ALMEIDA, NATALIA ELLEN; ESTEVES, FRANCIELE GREGO; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; DE PAULA, CARLA PERES; DA CUNHA, ANDERSON FERREIRA; MALAVAZI, IRAN; PALMA, MARIO SERGIO; RODRIGUES-FILHO, EDSON. Digestion of Intact Gluten Proteins by Bifidobacterium Species: Reduction of Cytotoxicity and Proinflammatory Responses. Journal of Agricultural and Food Chemistry, v. 68, n. 15, p. 4485-4492, . (16/16212-5, 17/10373-0, 15/26454-3, 17/19694-3)
SINGULANI, JUNYA DE LACORTE; GALEANE, MARIANA CRISTINA; RAMOS, MARINA DORISSE; GOMES, PAULO CESAR; DOS SANTOS, CLAUDIA TAVARES; DE SOUZA, BIBIANA MONSON; PALMA, MARIO SERGIO; FUSCO ALMEIDA, ANA MARISA; SOARES MENDES GIANNINI, MARIA JOSE. Antifungal Activity, Toxicity, and Membranolytic Action of a Mastoparan Analog Peptide. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 9, . (16/16212-5, 17/06658-9)
PRATAVIEIRA, MARCEL; DA SILVA MENEGASSO, ANALLY RIBEIRO; ROAT, THAISA; MALASPINA, OSMAR; PALMA, MARIO SERGIO. In Situ Metabolomics of the Honeybee Brain: The Metabolism of L-Arginine through the Polyamine Pathway in the Proboscis Extension Response (PER). JOURNAL OF PROTEOME RESEARCH, v. 19, n. 2, p. 832-844, . (16/16212-5)
APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ARCURI, HELEN ANDRADE; ESTEVES, FRANCIELE GREGO; PALMA, MARIO SERGIO; LUBEC, GERT. Spider silk proteome provides insight into the structural characterization of Nephila clavipes flagelliform spidroin. SCIENTIFIC REPORTS, v. 8, . (16/16212-5, 10/19051-6, 13/26451-9, 11/51684-1, 15/14220-8)
APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; PEREZ-RIVEROL, AMILCAR; MUSACCHIO LASA, ALEXIS; PALMA, MARIO SERGIO. Diversity of peptidic and proteinaceous toxins from social Hymenoptera venoms. Toxicon, v. 148, p. 172-196, . (17/22405-3, 16/16212-5)
PEREZ-RIVEROL, AMILCAR; LASA, ALEXIS MUSACCHIO; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; PALMA, MARIO SERGIO. Insect venom phospholipases A1 and A2: Roles in the envenoming process and allergy. Insect Biochemistry and Molecular Biology, v. 105, p. 10-24, . (17/22405-3, 16/16212-5)
SANTANA MARTINS, INGRID BERNARDES; VIEGAS, TAISA GIORDANO; ALVARES, DAYANE DOS SANTOS; DE SOUZA, BIBIANA MONSON; PALMA, MARIO SERGIO; NETO, JOAO RUGGIERO; DE ARAUJO, ALEXANDRE SUMAN. The effect of acidic pH on the adsorption and lytic activity of the peptides Polybia-MP1 and its histidine-containing analog in anionic lipid membrane: a biophysical study by molecular dynamics and spectroscopy. Amino Acids, v. 53, n. 5, p. 753-767, . (15/25619-9, 16/16212-5, 15/25620-7, 10/18169-3)
JAKOB, THILO; RAUBER, MICHELE MYRIAM; PEREZ-RIVEROL, AMILCAR; SPILLNER, EDZARD; BLANK, SIMON. The Honeybee Venom Major Allergen Api m 10 (Icarapin) and Its Role in Diagnostics and Treatment of Hymenoptera Venom Allergy. CURRENT ALLERGY AND ASTHMA REPORTS, v. 20, n. 9, . (17/22405-3, 16/16212-5, 18/24834-1)
APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ESTEVES, FRANCIELE GREGO; SIALANA, FERNANDO J.; FERRO, MILENE; SMIDAK, ROMAN; RARES, LUCACIU CALIN; NUSSBAUMER, THOMAS; RATTEI, THOMAS; BILBAN, MARTIN; BACCI JUNIOR, MAURICIO; et al. A proteotranscriptomic study of silk-producing glands from the orb-weaving spiders. MOLECULAR OMICS, v. 15, n. 4, p. 256-270, . (16/16212-5, 16/10430-0, 13/26451-9, 17/10373-0, 15/14220-8)
MARTINS, DANUBIA BATISTA; PACCA, CAROLINA COLOMBELLI; DA SILVA, ANNIELLE MENDES BRITO; DE SOUZA, BIBIANA MONSON; DE ALMEIDA, MARGARETE TERESA GOTTARDO; PALMA, MARIO SERGIO; ARCISIO-MIRANDA, MANOEL; DOS SANTOS CABRERA, MARCIA PEREZ. Comparing activity, toxicity and model membrane interactions of Jelleine-I and Trp/Arg analogs: analysis of peptide aggregation. Amino Acids, v. 52, n. 5, . (16/16212-5, 16/13368-4, 16/17951-6, 14/08372-7, 12/24259-0)
DA SILVA MENEGASSO, ANAILY RIBEIRO; PRATAVIEIRA, MARCEL; DOS SANTOS, LUCILENE DELAZARI; LIMA, FLAVIO DE OLIVEIRA; MORAES, MARCELO PADOVANI T.; ZANINI, MARCO ANTONIO; PALMA, MARIO SERGIO. In Situ Proteomic Analysis of Glioblastoma Multiforme: A Translational Approach to Improve Prognostic/Diagnostic Routines. BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY, v. 7, n. 29, p. 64-83, . (16/16212-5)
COSTA-LEONARDO, ANA MARIA; DA SILVA, IAGO BUENO; POIANI, SILVANA BEANI; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; ESTEVES, FRANCIELE GREGO; BUENO DA SILVA, LUIZA HELENA; PALMA, MARIO SERGIO. Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes. JOURNAL OF PROTEOMICS, v. 213, . (16/16212-5, 17/10373-0, 13/26451-9)
ROAT, THAISA C.; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; MIOTELO, LUCAS; DE SOUZA, CAROLINE LACERRA; PALMA, MARIO SERGIO; MALASPINA, OSMAR. Using a toxicoproteomic approach to investigate the effects of thiamethoxam into the brain of Apis mellifera. Chemosphere, v. 258, . (14/05376-1, 13/13542-6, 19/04538-1, 12/13370-8, 13/26451-9, 16/16212-5)
ESTEVES, FRANCIELE GREGO; APARECIDO DOS SANTOS-PINTO, JOSE ROBERTO; FERRO, MILENE; SIALANA, FERNANDO J.; SMIDAK, ROMAN; RARES, LUCACIU CALIN; NUSSBAUMER, THOMAS; RATTEI, THOMAS; BILBAN, MARTIN; BACCI JUNIOR, MAURICIO; et al. Revealing the Venomous Secrets of the Spider's Web. JOURNAL OF PROTEOME RESEARCH, v. 19, n. 8, p. 3044-3059, . (15/14220-8, 17/10373-0, 13/26451-9, 16/10430-0, 16/16212-5)
COSTA-LEONARDO, ANA MARIA; DA SILVA, IAGO BUENO; JANEI, VANELIZE; POIANI, SILVANA BEANI; DOS SANTOS-PINTO, JOSE ROBERTO APARECIDO; ESTEVES, FRANCIELE GREGO; PALMA, MARIO SERGIO. Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach. Cell and Tissue Research, v. 385, n. 3, p. 603-621, . (17/10373-0, 16/16212-5)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.