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INVESTIGATION OF THE PRESENCE OF EPSTEIN-BARR VIRUS AND ITS POSSIBLE CONTRIBUTION TO LARYNGEAL PAPILLOMATOSIS AGGRESSIVENESS AND MALIGNIZATION

Abstract

Introduction: Laryngeal papillomatosis is a benign neoplasia divided into two groups: juvenile and adult laryngeal papillomatosis. It is caused by the human papillomavirus (HPV). HPV types 6 and 11 are related to its etiology; however, the possible viral co-infection has been suggested in lesions of the head and neck region. Objectives: To describe the clinical and microscopic characteristics of juvenile and adult laryngeal papillomatosis; to genotype HPVs and comparing them with samples from Guatemala and Brazil, to investigate the presence of EBV-DNA by polymerase chain reaction (PCR) and EBV-RNA by in situ hybridization (ISH); to validate the presence of EBV through the immunoexpression of CD21, and to correlate the clinical-pathological results with the clinical and laryngoscopic scale by Derkay et al., 1998. Materials and Methods: 80 juvenile papillomatosis of paraffin blocks will be retrospectively reviewed through the slides of H&E. They will subdivided into 2 groups: low and high malignization risk based on Derkay index. The presence of HPV DNA in these specimens will be analyzed by polymerase chain reaction (PCR) using SPF10 primers (gene L1), it will be performed the hybridization of DNA strands for HPV genotyping. The DNA-EBV investigation will be performed through PCR for primers for the BamH1W segment. In situ hybridization for EBV-RNA (EBER) will be performed. The validation of the results for EBV will be realized by immunohistochemical reaction against-CD21. Clinical-pathological and molecular characteristics will be compared between groups using the Chi-square test or Fisher's exact test with a significance level of 5%. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
COSTA, VICTOR; EL-ACHKAR, VIVIAN NARANA; DE BARROS, PATRICIA PIMENTEL; LEON, JORGE ESQUICHE; RIBEIRO-SILVA, ALFREDO; CARLOS, ROMAN; NAGATA PIGNATARI, SHIRLEY SHIZUE; FERREIRA, SILVANEIDE; MELLO, BARBARA PEREIRA; SICHERO, LAURA; et al. Role of epstein-barr virus in the severity of recurrent respiratory papillomatosis. LARYNGOSCOPE, v. 130, n. 11, . (16/24019-0)
RIBEIRO EL ACHKAR, VIVIAN NARANA; DUARTE, ANDRESSA; CARLOS, ROMAN; LEON, JORGE ESQUICHE; RIBEIRO-SILVA, ALFREDO; NAGATA PIGNATARI, SHIRLEY SHIZUE; KAMINAGAKURA, ESTELA. Histopathological features of juvenile-onset laryngeal papillomatosis related to severity. HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK, v. 41, n. 5, p. 1412-1417, . (16/24019-0)
SICHERO, LAURA; FERREIRA, SILVANEIDE; LOPEZ, ROSSANA V. M.; MELLO, BARBARA P.; COSTA, VICTOR; EL-ACHKAR, VIVIAN N. R.; CARLOS, ROMAN; RIBEIRO-SILVA, ALFREDO; PIGNATARI, SHIRLEY; KAMINAGAKURA, ESTELA; et al. Prevalence of human papillomavirus 6 and 11 variants in recurrent respiratory papillomatosis. Journal of Medical Virology, v. 93, n. 6, p. 3835-3840, . (17/23211-8, 16/24019-0)
RIBEIRO EL-ACHKAR, VIVIAN NARANA; DUARTE, ANDRESSA; SAGGIORO, FABIANO PINTO; VERISSIMO DE MELLO FILHO, FRANCISCO; LEON, JORGE ESQUICHE; RIBEIRO-SILVA, ALFREDO; KAMINAGAKURA, ESTELA. Squamous Cell Carcinoma Originating from Adult Laryngeal Papillomatosis: Case Report and Review of the Literature. CASE REPORTS IN OTOLARYNGOLOGY, v. 2018, p. 5-pg., . (16/24019-0)
RIBEIRO EL-ACHKAR, VIVIAN NARANA; DUARTE, ANDRESSA; SAGGIORO, FABIANO PINTO; VERISSIMO DE MELLO FILHO, FRANCISCO; LEON, JORGE ESQUICHE; RIBEIRO-SILVA, ALFREDO; KAMINAGAKURA, ESTELA. Squamous Cell Carcinoma Originating from Adult Laryngeal Papillomatosis: Case Report and Review of the Literature. CASE REPORTS IN OTOLARYNGOLOGY, . (16/24019-0)
RIBEIRO EL ACHKAR, VIVIAN NARANA; DUARTE, ANDRESSA; CARLOS, ROMAN; LEON, JORGE ESQUICHE; RIBEIRO-SILVA, ALFREDO; NAGATA PIGNATARI, SHIRLEY SHIZUE; KAMINAGAKURA, ESTELA. Relationship between inflammation and the severity of Recurrent Respiratory Papillomatosis. AMERICAN JOURNAL OF OTOLARYNGOLOGY, v. 41, n. 2, . (16/24019-0)

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