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Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation

Grant number: 15/13684-0
Support Opportunities:Research Projects - Thematic Grants
Duration: March 01, 2017 - February 28, 2023
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal Investigator:Igor Polikarpov
Grantee:Igor Polikarpov
Host Institution: Instituto de Física de São Carlos (IFSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Pesquisadores principais:
Francisco Eduardo Gontijo Guimaraes
Associated researchers:Alessandro Silva Nascimento ; Camila Alves de Rezende ; Eduardo Ribeiro de Azevedo ; Marcos Silveira Buckeridge ; Munir Salomao Skaf
Associated grant(s):18/22300-0 - Synergistic activities to boost enzymatic lignocellulose oxidation using light and LPMOs, AP.R SPRINT
Associated scholarship(s):21/13009-2 - STRUCTURAL AND FUNCTIONAL STUDIES OF ENZYMES PARTICPATE IN COMPLEX CARBOHYDRATES SYNTHESIS AND DEGRADATION, BP.PD
20/15588-7 - Structural and functional studies of enzymes participate in complex carbohydrates synthesis and degradation, BP.TT
19/19360-3 - Plant cell wall-inspired nanocomposite hydrogels for biomedical applications, BP.DR
+ associated scholarships 19/21116-3 - Structural and functional studies of enzymes participate in complex carbohydrates synthesis and degradation, BP.TT
18/24705-7 - Structural and functional studies of enzymes participation in complex carbohydrates synthesis and degradation, BP.PD
19/10942-0 - Heterologous expression and characterization of xilanases that belong to GH10 and GH11 families and evaluation of its application in xilan cleavage leading to xylooligossacharides production from lignocelulosic biomass, BP.IC
18/00082-0 - Structural and biochemical studies of enzymes involved in the degradation of glucuronoarabinoxylan (GAX), BP.IC
18/02028-3 - Structural and functional studies of enzymes participate in complex carbohydrates synthesis and degradation, BP.TT
17/20706-6 - Structural and functional studies of enzymes that participate in complex carbohydrates synthesis and degradation, BP.TT - associated scholarships

Abstract

Carbohydrates, such as mono- and oligosaccharides, polysaccharides and glycoconjugates, play extremely important roles in all forms of life, participating in host-pathogen interactions, signal transduction, inflammation, intracellular trafficking and differentiation. In addition, carbohydrates make up about three quarters of renewable plant biomass (~ 135 billion tons/year) whose cell walls and reserve carbohydrates represent a sustainable source of energy and renewable materials. However, the diversity of carbohydrates and the complexity of the plant cell walls turn their enzymatic hydrolysis into simple sugars for biofuel production and "green" chemicals into a difficult task. Our current understanding of synthesis and enzymatic degradation of complex carbohydrates in the cell wall context are at least incomplete. Less than 3.8% of enzymes and auxiliary proteins involved in synthesis and degradation of carbohydrates are currently characterized biochemically and less than 0.42% of them are characterized structurally (Lombard et al., Nucl. Acids Res. (2014) 42: D490 - D495). Therefore, in this project we propose to conduct a systematic structural and functional study of the enzymes with potential in depolymerization and, also synthesis of polysaccharides, aiming to understand their specific mechanisms of action in the context of cell wall and also against isolated carbohydrates. The results of this study will shed light on the fundamental questions about enzymatic degradation of biopolymers and may be important for biotechnological applications such as enzymatic hydrolysis of lignocellulosic materials and their transformation in platform molecules and biofuels, among others. (AU)

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Scientific publications (61)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VELDMAN, WAYDE; LIBERATO, MARCELO VIZONA; ALMEIDA, VITOR M.; SOUZA, VALQUIRIA P.; FRUTUOSO, MAIRA A.; MARANA, SANDRO R.; MOSES, VUYANI; BISHOP, OZLEM TASTAN; POLIKARPOV, IGOR. X-ray Structure, Bioinformatics Analysis, and Substrate Specificity of a 6-Phospho-beta-glucosidase Glycoside Hydrolase 1 Enzyme from Bacillus licheniformis. JOURNAL OF CHEMICAL INFORMATION AND MODELING, v. 60, n. 12, p. 6392-6407, . (15/13684-0)
DE ARAUJO, EVANDRO ARES; DE OLIVEIRA NETO, MARIO; POLIKARPOV, IGOR. Biochemical characterization and low-resolution SAXS structure of two-domain endoglucanase BlCel9 from Bacillus licheniformis. Applied Microbiology and Biotechnology, v. 103, n. 3, p. 1275-1287, . (15/13684-0)
VELDMAN, WAYDE; LIBERATO, MARCELO VIZONA; SOUZA, VALQUIRIA P.; ALMEIDA, VITOR M.; MARANA, SANDRO R.; BISHOP, OZLEM TASTAN; POLIKARPOV, IGOR. Differences in Gluco and Galacto Substrate-Binding Interactions in a Dual 6P beta-Glucosidase/6P beta-Galactosidase Glycoside Hydrolase 1 Enzyme from Bacillus licheniformis. JOURNAL OF CHEMICAL INFORMATION AND MODELING, v. 61, n. 9, p. 4554-4570, . (15/13684-0)
SARTORI, GERALDO RODRIGUES; NASCIMENTO, ALESSANDRO S.. Comparative Analysis of Electrostatic Models for Ligand Docking. FRONTIERS IN MOLECULAR BIOSCIENCES, v. 6, . (15/13684-0, 17/18173-0, 15/26722-8)
DE AZEVEDO, ERIKA CHANG; NASCIMENTO, ALESSANDRO S.. Energy landscape of the domain movement in Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase. Journal of Structural Biology, v. 207, n. 2, p. 158-168, . (15/13684-0, 17/18173-0, 15/26722-8)
DO ESPIRITO SANTO, MELISSA CRISTINA; CARDOSO, ELIANO BRITO; GONTIJO GUIMARAES, FRANCISCO EDUARDO; DEAZEVEDO, EDUARDO RIBEIRO; DA CUNHA, GIOVANNI PARO; NOVOTNY, ETELVINO HENRIQUE; ARNOLDI PELLEGRINI, VANESSA DE OLIVEIRA; CHANDEL, ANUJ KUMAR; LUCIANO SILVEIRA, MARCOS HENRIQUE; POLIKARPOV, IGOR. Multifaceted characterization of sugarcane bagasse under different steam explosion severity conditions leading to distinct enzymatic hydrolysis yields. INDUSTRIAL CROPS AND PRODUCTS, v. 139, . (15/13684-0)
FARRO, ERICK GIANCARLO S.; LEITE, ANA ELISA T.; SILVA, ISABELA A.; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; POLIKARPOV, IGOR; NASCIMENTO, ALESSANDRO S.. GH43 endo-arabinanase from Bacillus licheniformis: Structure, activity and unexpected synergistic effect on cellulose enzymatic hydrolysis. International Journal of Biological Macromolecules, v. 117, p. 7-16, . (14/06565-2, 15/13684-0, 14/03768-0)
BESSER, KATRIN; MALYON, GRAHAM P.; EBORALL, WILLIAM S.; DA CUNHA, GIOVANNI PARO; FILGUEIRAS, JEFFERSON G.; DOWLE, ADAM; GARCIA, LOURDES CRUZ; PAGE, SAMUEL J.; DUPREE, RAY; KERN, MARCELO; et al. Hemocyanin facilitates lignocellulose digestion by wood-boring marine crustaceans. NATURE COMMUNICATIONS, v. 9, . (15/13684-0, 17/24465-3)
BERTO, GABRIELA LEILA; VELASCO, JOSMAN; CABOS RIBEIRO, CAIO TASSO; ZANPHORLIN, LETICIA MARIA; DOMINGUES, MARIANE NORONHA; MURAKAMI, MARIO TYAGO; POLIKARPOV, IGOR; DE OLIVEIRA, LEANDRO CRISTANTE; FERRAZ, ANDRE; SEGATO, FERNANDO. Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45. Enzyme and Microbial Technology, v. 120, p. 23-35, . (14/06923-6, 14/18714-2, 15/13684-0, 15/26982-0)
VACILOTTO, MILENA MOREIRA; ARNOLDI PELLEGRINI, VANESSA O.; VEIGA SEPULCHRO, ANA GABRIELA; DE MELLO CAPETTI, CAIO C.; CURVELO, ANTONIO APRIGIO S.; MARCONDES, WILIAN FIORELI; ARANTES, VALDEIR; POLIKARPOV, IGOR. Paludibacter propionicigenes GH10 xylanase as a tool for enzymatic xylooligosaccharides production from heteroxylans. Carbohydrate Polymers, v. 275, . (15/13684-0)
VIEIRA, PLINIO S.; BONFIM, ISABELA M.; ARAUJO, EVANDRO A.; MELO, RICARDO R.; LIMA, AUGUSTO R.; FESSEL, MELISSA R.; PAIXAO, DOUGLAS A. A.; PERSINOTI, GABRIELA F.; ROCCO, SILVANA A.; LIMA, TATIANI B.; et al. Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors. NATURE COMMUNICATIONS, v. 12, n. 1, . (17/14253-9, 15/26982-0, 16/06509-0, 15/13684-0, 18/03724-3, 19/13936-0, 17/00203-0, 16/19995-0)
KADOWAKI, MARCO A. S.; BRIGANTI, LORENZO; EVANGELISTA, DANILO E.; ECHEVARRIA-POZA, ALBERTO; TRYFONA, THEODORA; PELLEGRINI, VANESSA O. A.; NAKAYAMA, DARLAN G.; DUPREE, PAUL; POLIKARPOV, IGOR. Unlocking the structural features for the xylobiohydrolase activity of an unusual GH11 member identified in a compost-derived consortium. Biotechnology and Bioengineering, v. 118, n. 10, . (15/13684-0)
DE AZEVEDO, ERIKA CHANG; NASCIMENTO, ALESSANDRO S.. The beta-lactam ticarcillin is a Staphylococcus aureus UDP-N-acetylglucosamine 2-epimerase binder. Biochimie, v. 197, p. 8-pg., . (17/18173-0, 15/26722-8, 15/13684-0, 20/03983-9)
KANE, AISSATA OUSMANE; PELLERGINI, VANESSA O. ARNOLDI; ESPIRITO SANTO, MELISSA C.; NGOM, BALLA DIOP; GARCIA, JOSE M.; ACEVEDO, ALBERTO; ERAZZU, LUIS E.; POLIKARPOV, IGOR. valuating the Potential of Culms from Sugarcane and Energy Cane Varieties Grown in Argentina for Second-Generation Ethanol Productio. WASTE AND BIOMASS VALORIZATION, v. 13, n. 1, SI, . (15/13684-0)
BARRA, ANGELICA LUANA C.; ULLAH, NAJEEB; MORAO, LUANA G.; WRENGER, CARSTEN; BETZEL, CHRISTIAN; NASCIMENTO, ALESSANDRO S.. Structural Dynamics and Perspectives of Vitamin B6 Biosynthesis Enzymes in Plasmodium: Advances and Open Questions. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, . (18/21213-6, 19/20219-3, 20/03983-9, 15/13684-0, 19/26428-3, 15/26722-8, 17/03966-4)
EVANGELISTA, DANILO ELTON; DE ARAUJO, EVANDRO A.; OLIVEIRA NETO, MARIO; SEIKI KADOWAKI, MARCO ANTONIO; POLIKARPOV, IGOR. Biochemical characterization and low-resolution SAXS structure of an exopolygalacturonase from Bacillus licheniformis. NEW BIOTECHNOLOGY, v. 40, n. B, p. 268-274, . (11/20505-4, 15/13684-0)
MELLO, BRUNO L.; ALESSI, ANNA M.; RIANO-PACHON, DIEGO M.; DEAZEVEDO, EDUARDO R.; GUIMARAES, FRANCISCO E. G.; ESPIRITO SANTO, MELISSA C.; MCQUEEN-MASON, SIMON; BRUCE, NEIL C.; POLIKARPOV, IGOR. Targeted metatranscriptomics of compost-derived consortia reveals a GH11 exerting an unusual exo-1,4-beta-xylanase activity. BIOTECHNOLOGY FOR BIOFUELS, v. 10, . (11/21608-1, 10/52362-5, 15/13684-0)
SEPULCHRO, V, ANA GABRIELA; PELLEGRINI, VANESSA O. A.; DIAS, LUCAS D.; KADOWAKI, MARCO A. S.; CANNELLA, DAVID; POLIKARPOV, IGOR. Combining pieces: a thorough analysis of light activation boosting power and co-substrate preferences for the catalytic efficiency of lytic polysaccharide monooxygenase MtLPMO9A. BIOFUEL RESEARCH JOURNAL-BRJ, v. 8, n. 3, p. 1454-1464, . (18/22300-0, 15/13684-0, 19/13569-8)
SEIKI KADOWAKI, MARCO ANTONIO; RABELO HIGASI, PAULA MIWA; DE GODOY, MARIANA ORTIZ; DE ARAUJO, EVANDRO ARES; GODOY, ANDRE SCHUTZER; PRADE, ROLF ALEXANDER; POLIKARPOV, IGOR. Enzymatic versatility and thermostability of a new aryl-alcohol oxidase from Thermothelomyces thermophilus M77. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1864, n. 10, . (15/13684-0, 11/20505-4)
HANS, MEENU; KUMAR, SACHIN; CHANDEL, ANUJ KUMAR; POLIKARPOV, IGOR. A review on bioprocessing of paddy straw to ethanol using simultaneous saccharification and fermentation. Process Biochemistry, v. 85, p. 125-134, . (15/13684-0)
EVANGELISTA, DANILO ELTON; SEIKI KADOWAKI, MARCO ANTONIO; MELLO, BRUNO LUAN; POLIKARPOV, IGOR. Biochemical and biophysical characterization of novel GH10 xylanase prospected from a sugar cane bagasse compost-derived microbial consortia. International Journal of Biological Macromolecules, v. 109, p. 560-568, . (11/20505-4, 11/21608-1, 10/52362-5, 15/13684-0)
ARNOLDI PELLEGRINI, VANESSA DE OLIVEIRA; VEIGA SEPULCHRO, ANA GABRIELA; POLIKARPOV, IGOR. Enzymes for lignocellulosic biomass polysaccharide valorization and production of nanomaterials. CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY, v. 26, . (15/13684-0)
PELLEGRINI, VANESSA DE OLIVEIRA ARNOLDI; RATTI, REGIANE PRISCILA; FILGUEIRAS, JEFFERSON GONCALVES; FALVO, MAURICIO; CORAL, MARISA APARECIDA LIMA; GUIMARAES, FRANCISCO EDUARDO GONTIJO; DEAZEVEDO, EDUARDO RIBEIRO; POLIKARPOV, IGOR. Differences in chemical composition and physical properties caused by industrial storage on sugarcane bagasse result in its efficient enzymatic hydrolysis. SUSTAINABLE ENERGY & FUELS, v. 6, n. 2, . (15/13684-0)
KANE, AISSATA OUSMANE; CORTEZ, ANELYSE ABREU; PELLEGRINI, VANESSA OLIVEIRA ARNOLDI; NGOM, BALLA DIOP; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; POLIKARPOV, IGOR. Combined liquid hot water and sulfonation pretreatment of sugarcane bagasse to maximize fermentable sugars production. INDUSTRIAL CROPS AND PRODUCTS, v. 201, p. 13-pg., . (15/13684-0, 21/08780-1)
HANS, MEENU; PELLEGRINI, VANESSA O. A.; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; GUIMARAES, FRANCISCO E. C.; CHANDEL, ANUJ KUMAR; POLIKARPOV, IGOR; CHADHA, BHUPINDER SINGH; KUMAR, SACHIN. Optimization of Dilute Acid Pretreatment for Enhanced Release of Fermentable Sugars from Sugarcane Bagasse and Validation by Biophysical Characterization. BioEnergy Research, v. N/A, p. 19-pg., . (15/13684-0)
ESPIRITO SANTO, MELISSA C.; KANE, AISSATA OUSMANE; PELLEGRINI, VANESSA O. A.; THEMA, FORCE TEFO; GARCIA, JOSE M.; ACEVEDO, ALBERTO; ERAZZU, LUIS E.; GUIMARAES, FRANCISCO E. G.; DEAZEVEDO, EDUARDO R.; POLIKARPOV, IGOR. Leaves from four different sugarcane varieties as potential renewable feedstocks for second-generation ethanol production: Pretreatments, chemical composition, physical structure, and enzymatic hydrolysis yields. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 45, p. 13-pg., . (15/13684-0)
SEIKI KADOWAKI, MARCO ANTONIO; DE GODOY, MARIANA ORTIZ; KUMAGAI, PATRICIA SUEMY; DA COSTA-FILHO, ANTONIO JOSE; MORT, ANDREW; PRADE, ROLF ALEXANDER; POLIKARPOV, IGOR. Characterization of a New Glyoxal Oxidase from the Thermophilic Fungus Myceliophthora thermophila M77: Hydrogen Peroxide Production Retained in 5-Hydroxymethylfurfural Oxidation. CATALYSTS, v. 8, n. 10, . (15/13684-0, 11/20505-4)
KADOWAKI, MARCO A. S.; HIGASI, PAULA; DE GODOY, MARIANA O.; PRADE, ROLF A.; POLIKARPOV, IGOR. Biochemical and structural insights into a thermostable cellobiohydrolase from Myceliophthora thermophila. FEBS Journal, v. 285, n. 3, p. 559-579, . (09/52840-7, 15/13684-0, 11/20505-4)
GODOY, ANDRE S.; PEREIRA, CAROLINE S.; RAMIA, MARINA PAGLIONE; SILVEIRA, RODRIGO L.; CAMILO, CESAR M.; KADOWAKI, MARCO A.; LANGE, LENE; BUSK, PETER K.; NASCIMENTO, ALESSANDRO S.; SKAF, MUNIR S.; et al. Structure, computational and biochemical analysis of PcCel45A endoglucanase from Phanerochaete chrysosporium and catalytic mechanisms of GH45 subfamily C members. SCIENTIFIC REPORTS, v. 8, . (15/13684-0, 11/21608-1, 10/52362-5, 11/20505-4)
ROSSI, BRUNO R.; PELLEGRINI, VANESSA O. A.; CORTEZ, ANELYSE A.; CHIROMITO, EMANOELE M. S.; CARVALHO, ANTONIO J. F.; PINTO, LIDIANE O.; REZENDE, CAMILA A.; MASTELARO, VALMOR R.; POLIKARPOV, IGOR. Cellulose nanofibers production using a set of recombinant enzymes. Carbohydrate Polymers, v. 256, . (15/13684-0)
VACILOTTO, MILENA MOREIRA; SEPULCHRO, ANA GABRIELA VEIGA; PELLEGRINI, VANESSA O. A.; POLIKARPOV, IGOR. Production of prebiotic xylooligosaccharides from arabino- and glucuronoxylan using a two-domain Jonesia denitrificans xylanase from GH10 family. Enzyme and Microbial Technology, v. 144, . (15/13684-0)
VELASCO, JOSMAN; PELLEGRINI, VANESSA DE OLIVEIRA ARNOLDI; SEPULCHRO, ANA GABRIELA VEIGA; KADOWAKI, MARCO ANTONIO SEIKI; SANTO, MELISSA CRISTINA ESPIRITO; POLIKARPOV, IGOR; SEGATO, FERNANDO. Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification. Enzyme and Microbial Technology, v. 144, . (19/22284-7, 15/13684-0, 14/06923-6, 14/18714-2, 19/01165-0, 17/50025-0)
HANS, MEENU; GARG, SHRUTI; PELLEGRINI, VANESSA O. A.; FILGUEIRAS, JEFFERSON G.; DE AZEVEDO, EDUARDO R.; GUIMARAES, FRANCISCO E. C.; CHANDEL, ANUJ KUMAR; POLIKARPOV, IGOR; CHADHA, BHUPINDER SINGH; KUMAR, SACHIN. Liquid ammonia pretreatment optimization for improved release of fermentable sugars from sugarcane bagasse. JOURNAL OF CLEANER PRODUCTION, v. 281, . (15/13684-0)
BERNARDES, A.; PELLEGRINI, V. O. A.; CURTOLO, F.; CAMILO, C. M.; MELLO, B. L.; JOHNS, M. A.; SCOTT, J. L.; GUIMARAES, F. E. C.; POLIKARPOV, I.. Carbohydrate binding modules enhance cellulose enzymatic hydrolysis by increasing access of cellulases to the substrate. Carbohydrate Polymers, v. 211, p. 57-68, . (10/18773-8, 15/13684-0)
PIIADOV, VASSILI; DE ARAUJO, EVANDRO ARES; OLIVEIRA NETO, MARIO; CRAIEVICH, ALDO FELIX; POLIKARPOV, IGOR. SAXSMoW 2.0: Online calculator of the molecular weight of proteins in dilute solution from experimental SAXS data measured on a relative scale. Protein Science, v. 28, n. 2, p. 454-463, . (14/00769-5, 15/13684-0)
SANTO, MELISSA ESPIRITO; REZENDE, CAMILA A.; BERNARDINELLI, OIGRES D.; PEREIRA, JR., NEI; CURVELO, ANTONIO A. S.; DEAZEVEDO, EDUARDO R.; GUIMARAES, FRANSCISCO E. G.; POLIKARPOV, IGOR. Structural and compositional changes in sugarcane bagasse subjected to hydrothermal and organosolv pretreatments and their impacts on enzymatic hydrolysis. INDUSTRIAL CROPS AND PRODUCTS, v. 113, p. 64-74, . (10/52362-5, 15/13684-0)
PELLEGRINI, VANESSA O. A.; BERNARDES, AMANDA; REZENDE, CAMILA A.; POLIKARPOV, IGOR. Cellulose fiber size defines efficiency of enzymatic hydrolysis and impacts degree of synergy between endo- and exoglucanases. Cellulose, v. 25, n. 3, p. 1865-1881, . (12/22802-9, 10/18773-8, 15/13684-0, 16/13602-7)
DE ARAUJO, EVANDRO ARES; MANZINE, LIVIA REGINA; PIIADOV, VASSILI; SEIKI KADOWAKI, MARCO ANTONIO; POLIKARPOV, IGOR. Biochemical characterization, low-resolution SAXS structure and an enzymatic cleavage pattern of BlCel48 from Bacillus licheniformis. International Journal of Biological Macromolecules, v. 111, p. 302-310, . (14/00769-5, 11/20505-4, 10/52362-5, 15/13684-0)
SONODA, MILTON T.; GODOY, ANDRE S.; PELLEGRINI, VANESSA O. A.; KADOWAKI, MARCO A. S.; NASCIMENTO, ALESSANDRO S.; POLIKARPOV, IGOR. Structure and dynamics of Trichoderma harzianum Cel7B suggest molecular architecture adaptations required for a wide spectrum of activities on plant cell wall polysaccharides. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, v. 1863, n. 6, p. 1015-1026, . (14/06565-2, 15/13684-0, 17/18173-0)
KADOWAKI, MARCO A. S.; VARNAI, ANIKO; JAMESON, JOHN-KRISTIAN; LEITE, ANA E. T.; COSTA-FILHO, ANTONIO J.; KUMAGAI, PATRICIA S.; PRADE, ROLF A.; POLIKARPOV, IGOR; EIJSINK, VINCENT G. H.. Functional characterization of a lytic polysaccharide monooxygenase from the thermophilic fungus Myceliophthora thermophila. PLoS One, v. 13, n. 8, . (11/20505-4, 15/13684-0)
SEPULCHRO, ANA GABRIELA VEIGA; PELLEGRINI, VANESSA O. A.; BRIGANTI, LORENZO; DE ARAUJO, EVANDRO A.; DE ARAUJO, SIMARA S.; POLIKARPOV, IGOR. Transformation of xylan into value-added biocommodities using Thermobacillus composti GH10 xylanase. Carbohydrate Polymers, v. 247, . (15/13684-0)
KELLER, M. B.; FELBY, C.; LABATE, C. A.; PELLEGRINI, V. O. A.; HIGASI, P.; SINGH, R. K.; POLIKARPOV, I.; BLOSSOM, B. M.. A simple enzymatic assay for the quantification of C1-specific cellulose oxidation by lytic polysaccharide monooxygenases. Biotechnology Letters, v. 42, n. 1, p. 93-102, . (17/01165-4, 15/13684-0)
ARAUJO, EVANDRO A.; DIAS, ARTUR HERMANO SAMPAIO; KADOWAKI, MARCO A. S.; PIYADOV, VASILY; PELLEGRINI, VANESSA O. A.; URIO, MATEUS B.; RAMOS, LUIZ P.; SKAF, MUNIR S.; POLIKARPOV, IGOR. mpact of cellulose properties on enzymatic degradation by bacterial GH48 enzymes: Structural and mechanistic insights from processive Bacillus licheniformis Cel48B cellulas. Carbohydrate Polymers, v. 264, . (13/08293-7, 15/13684-0, 14/00769-5, 19/17350-0)
EVANGELISTA, DANILO ELTON; ARNOLDI PELLEGRIN, VANESSA DE OLIVEIRA; SANTO, MELISSA ESPIRITO; MCQUEEN-MASON, SIMON; BRUCE, NEIL C.; POLIKARPOV, IGOR. Biochemical characterization and low-resolution SAXS shape of a novel GH11 exo-1,4-beta-xylanase identified in a microbial consortium. Applied Microbiology and Biotechnology, v. 103, n. 19, p. 8035-8049, . (15/13684-0, 11/21608-1, 10/52362-5, 11/20505-4)
ESPIRITO SANTO, MELISSA C.; FOCKINK, DOUGLAS H.; PELLEGRINI, VANESSA O. A.; GUIMARAES, FRANCISCO E. G.; DEAZEVEDO, EDUARDO R.; RAMOS, LUIZ PEREIRA; POLIKARPOV, IGOR. Physical techniques shed light on the differences in sugarcane bagasse structure subjected to steam explosion pretreatments at equivalent combined severity factors. INDUSTRIAL CROPS AND PRODUCTS, v. 158, . (15/13684-0)
VELASCO, JOSMAN; SEPULCHRO, ANA GABRIELA V.; HIGASI, PAULA M. R.; PELLEGRINI, VANESSA O. A.; CANNELLA, DAVID; DE OLIVEIRA, LEANDRO CRISTANTE; POLIKARPOV, IGOR; SEGATO, FERNANDO. Light Boosts the Activity of Novel LPMO from Aspergillus fumigatus Leading to Oxidative Cleavage of Cellulose and Hemicellulose. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 10, n. 50, p. 16-pg., . (19/22284-7, 18/22300-0, 19/01165-0, 21/06679-1, 14/18714-2, 15/13684-0)
TOMAZINI, ATILIO; HIGASI, PAULA; MANZINE, LIVIA R.; STOTT, MATTHEW; SPARLING, RICHARD; LEVIN, DAVID B.; POLIKARPOV, IGOR. A novel thermostable GH5 beta-xylosidase from Thermogemmatispora sp. T81. NEW BIOTECHNOLOGY, v. 53, p. 57-64, . (15/13684-0)
PUHL, ANA C.; PRATES, ERICA T.; ROSSETO, FLAVIO R.; MANZINE, LIVIA R.; STANKOVIC, IVANA; DE ARAUJO, SIMARA S.; ALVAREZ, THABATA M.; SQUINA, FABIO M.; SKAF, MUNIR S.; POLIKARPOV, IGOR. Crystallographic structure and molecular dynamics simulations of the major endoglucanase from Xanthomonas campestris pv. campestris shed light on its oligosaccharide products release pattern. International Journal of Biological Macromolecules, v. 136, p. 493-502, . (11/20505-4, 15/13684-0, 09/52840-7, 10/52362-5, 11/21608-1, 15/50590-4)
BRIGANTI, LORENZO; CAPETTI, CAIO; PELLEGRINI, VANESSA O. A.; GHIO, SILVINA; CAMPOS, ELEONORA; NASCIMENTO, ALESSANDRO S.; POLIKARPOV, IGOR. Structural and molecular dynamics investigations of ligand stabilization via secondary binding site interactions in Paenibacillus xylanivorans GH11 xylanase. COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL, v. 19, p. 1557-1566, . (20/03983-9, 15/26722-8, 15/13684-0)
CAPETTI, CAIO CESAR DE MELLO; VACILOTTO, MILENA MOREIRA; DABUL, ANDREI NICOLI GEBIELUCA; SEPULCHRO, ANA GABRIELA VEIGA; PELLEGRINI, VANESSA OLIVEIRA ARNOLDI; POLIKARPOV, IGOR. Recent advances in the enzymatic production and applications of xylooligosaccharides. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v. 37, n. 10, . (15/13684-0)
BRAR, KAMALPREET KAUR; SANTO, MELISSA C. ESPIRITO; PELLEGRINI, VANESSA O. A.; DEAZEVEDO, EDUARDO R.; GUIMARAES, FRANCISCO E. C.; POLIKARPOV, IGOR; CHADHA, BHUPINDER SINGH. Enhanced hydrolysis of hydrothermally and autohydrolytically treated sugarcane bagasse and understanding the structural changes leading to improved saccharification. BIOMASS & BIOENERGY, v. 139, . (15/13684-0)
LEITE, ANA ELISA T.; BRIGANTI, LORENZO; DE ARAUJO, EVANDRO ARES; ARNOLDI PELLEGRINI, VANESSA DE OLIVEIRA; CAMILO, CESAR MOYSES; POLIKARPOV, IGOR. Low-resolution molecular shape, biochemical characterization and emulsification properties of a halotolerant esterase fromBacillus licheniformis. EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, v. 49, n. 6, . (15/13684-0)
HIGASI, PAULA M. R.; VELASCO, JOSMAN A.; PELLEGRINI, VANESSA O. A.; DE ARAUJO, EVANDRO A.; FRANCA, BRUNO ALVES; KELLER, MALENE B.; LABATE, CARLOS A.; BLOSSOM, BENEDIKT M.; SEGATO, FERNANDO; POLIKARPOV, IGOR. Light-stimulated T. thermophilus two-domain LPMO9H: Low-resolution SAXS model and synergy with cellulases. Carbohydrate Polymers, v. 260, . (15/13684-0, 17/01165-4)
DO ESPIRITO SANTO, MELISSA CRISTINA; THEMA, FORCE TEFO; ARNOLDI PELLEGRINI, VANESSA DE OLIVEIRA; KANE, AISSATA OUSMANE; GONTIJO GUIMARAES, FRANCISCO EDUARDO; FILGUEIRAS, JEFFERSON G.; NOVOTNY, ETELVINO HENRIQUE; DEAZEVEDO, EDUARDO RIBEIRO; POLIKARPOV, IGOR. When the order matters: Impacts of lignin removal and xylan conformation on the physical structure and enzymatic hydrolysis of sugarcane bagasse. INDUSTRIAL CROPS AND PRODUCTS, v. 180, p. 12-pg., . (15/13684-0)
CAPETTI, CAIO CESAR DE MELLO; PELLEGRINI, VANESSA OLIVEIRA ARNOLDI; SANTO, MELISSA CRISTINA ESPIRITO; CORTEZ, ANELYSE ABREU; FALVO, MAURICIO; CURVELO, ANTONIO APRIGIO DA SILVA; CAMPOS, ELEONORA; FILGUEIRAS, JEFFERSON GONSALVES; GUIMARAES, FRANCISCO EDUARDO GONTIJO; DE AZEVEDO, EDUARDO RIBEIRO; et al. Enzymatic production of xylooligosaccharides from corn cobs: Assessment of two different pretreatment strategies. Carbohydrate Polymers, v. 299, p. 12-pg., . (19/10942-0, 15/13684-0)
EVANGELISTA, DANILO ELTON; DE ARAUJO, EVANDRO A.; OLIVEIRA NETO, MARIO; SEIKI KADOWAKI, MARCO ANTONIO; POLIKARPOV, IGOR. Biochemical characterization and low-resolution SAXS structure of an exopolygalacturonase from Bacillus licheniformis. NEW BIOTECHNOLOGY, v. 40, p. 7-pg., . (15/13684-0, 11/20505-4)
ARAUJO, EVANDRO A.; DIAS, ARTUR HERMANO SAMPAIO; KADOWAKI, MARCO A. S.; PIYADOV, VASILY; PELLEGRINI, VANESSA O. A.; URIO, MATEUS B.; RAMOS, LUIZ P.; SKAF, MUNIR S.; POLIKARPOV, IGOR. Impact of cellulose properties on enzymatic degradation by bacterial GH48 enzymes: Structural and mechanistic insights from processive Bacillus licheniformis Cel48B cellulase. Carbohydrate Polymers, v. 264, p. 13-pg., . (14/00769-5, 13/08293-7, 15/13684-0, 19/17350-0)
NETO, MARIO DE OLIVEIRA; FERNANDES, ADRIANO DE FREITAS; PIIADOV, VASSILI; CRAIEVICH, ALDO FELIX; DE ARAUJO, EVANDRO ARES; POLIKARPOV, IGOR. SAXSMoW 3.0: New advances in the determination of the molecular weight of proteins in dilute solutions from SAXS intensity data on a relative scale. Protein Science, v. 31, n. 1, SI, . (18/22300-0, 15/13684-0)
BARRA, ANGELICA LUANA C.; DANTAS, LIVIA DE OLIVEIRA C.; MORAO, LUANA GALVAO; GUTIERREZ, RAISSA F.; POLIKARPOV, IGOR; WRENGER, CARSTEN; NASCIMENTO, ALESSANDRO S.. Essential Metabolic Routes as a Way to ESKAPE From Antibiotic Resistance. FRONTIERS IN PUBLIC HEALTH, v. 8, . (15/13684-0, 17/18173-0, 15/26722-8)

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