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Bioavailability of peptides from amaranth (Amaranthus cruentus L. BRS Alegria) and cowpea (Vigna unguiculata L. Walp) and their modulation of cholesterol biosynthesis and absorption

Grant number: 16/08758-8
Support Opportunities:Regular Research Grants
Duration: March 01, 2017 - February 28, 2019
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Science
Principal Investigator:José Alfredo Gomes Arêas
Grantee:José Alfredo Gomes Arêas
Host Institution: Faculdade de Saúde Pública (FSP). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers:Marcelo Rodrigues Marques

Abstract

Introduction: The diet plays an important role in cardiovascular disease and some foods, such as amaranth and cowpea, have proven ability to reduce serum cholesterol. This effect is related to peptides formed during digestion of their protein. Bioavailability and the fate of these peptides after digestion are clearly complex, and their target tissues are unknown. Objectives: To assess the bioavailability of peptides from amaranth and cowpea and verify their relationship to the absorption and metabolism of cholesterol. Methodology: Synthetic peptides will be used to verify gene expression related to cholesterol metabolism in Hep G2 cells. Besides, in vivo experiments will be conducted. In these experiments, animals will be fed protein isolated from the cowpea and from amaranth, and the concentration of target peptides will be monitored in the blood. Furthermore, there will be checked several metabolic aspects and gene expression of various receptors, transporters and enzymes in various tissues of animals fed hypercholesterolemic diets containing amaranth or cowpea proteins as unique feed protein sources. There will be determined in the plasma: cholesterol and fractions; in the faeces: cholesterol and bile acids; in the liver: cholesterol, total lipids, HMGR activity, and quantification of protein expression of HMGR, SREBP-2, NPC1L1, ABCCG5, ABCCG8, ABCA1, ABCG1, LDL receptors, PEPT1 receptors; and in the gut: protein expression and quantification of NPC1L1, ABCCG5, ABCCG8, ABCA1, ABCG1 and PEPT1 receptors. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GONCALVES FROTA, KAROLINE DE MACEDO; ROSAL LOPES, LAYS ARNAUD; VERAS SILVA, IZABEL CRISTINA; GOMES AREAS, JOSE ALFREDO. Nutritional quality of the protein of Vigna unguiculata L. Walp and its protein isolate. Rev. Ciênc. Agron., v. 48, n. 5, p. 792-798, . (12/15900-4, 16/08758-8)
KAROLINE DE MACÊDO GONÇALVES FROTA; LAYS ARNAUD ROSAL LOPES; IZABEL CRISTINA VERAS SILVA; JOSÉ ALFREDO GOMES ARÊAS. PERFIL MOLECULAR, PUREZA E ATIVIDADE DO INIBIDOR DE TRIPSINA EM ISOLADOS PROTEICOS DE FEIJÃO-CAUPI. Rev. Caatinga, v. 31, n. 1, p. 202-208, . (12/15900-4, 16/08758-8)
REGILDA SARAIVA DOS REIS MOREIRA-ARAÚJO; GENI RODRIGUES SAMPAIO; ROSANA APARECIDA MANÓLIO SOARES; CÍNTIA PEREIRA DA SILVA; MARCOS ANTONIO DA MOTA ARAÚJO; JOSÉ ALFREDO GOMES ARÊAS. IDENTIFICAÇÃO E QUANTIFICAÇÃO DE COMPOSTOS FENÓLICOS E DA ATIVIDADE ANTIOXIDANTE NO FEIJÃO-CAUPI EM GRÃOS DA CULTIVAR BRS XIQUEXIQUE. Rev. Caatinga, v. 31, n. 1, p. 209-216, . (16/08758-8)
GONCALVES FROTA, KAROLINE DE MACEDO; ROSAL LOPES, LAYS ARNAUD; VERAS SILVA, IZABEL CRISTINA; AREAS, ALFREDO GOMES. MOLECULAR PROFILE, PURITY AND PRESENCE OF TRYPSIN INHIBITORS IN COWPEA PROTEIN ISOLATES. REVISTA CAATINGA, v. 31, n. 1, p. 202-208, . (16/08758-8, 12/15900-4)
DOS REIS MOREIRA-ARAUJO, REGILDA SARAIVA; SAMPAIO, GENI RODRIGUES; MANOLIO SOARES, ROSANA APARECIDA; DA SILVA, CINTIA PEREIRA; DA MOTA ARAUJO, MARCOS ANTONIO; GOMES AREAS, JOSE ALFREDO. IDENTIFICATION AND QUANTIFICATION OF PHENOLIC COMPOUNDS AND ANTIOXIDANT ACTIVITY IN COWPEAS OF BRS XIQUEXIQUE CULTIVAR. REVISTA CAATINGA, v. 31, n. 1, p. 209-216, . (16/08758-8)
DOS REIS MOREIRA-ARAUJO, REGILDA SARAIVA; SAMPAIO, GENI RODRIGUES; MANOLIO SOARES, ROSANA APARECIDA; SILVA, CINTIA PEREIRA; GOMES AREAS, JOSE ALFREDO. Identification and quantification of antioxidant compounds in cowpea. Rev. Ciênc. Agron., v. 48, n. 5, p. 799-805, . (16/08758-8)
DOS REIS MOREIRA-ARAUJO, REGILDA SARAIVA; SAMPAIO, GENI RODRIGUES; MANOLIO SOARES, ROSANA APARECIDA; SILVA, CINTIA PEREIRA; GOMES AREAS, JOSE ALFREDO. Identification and quantification of antioxidant compounds in cowpea. REVISTA CIENCIA AGRONOMICA, v. 48, n. 5, p. 7-pg., . (16/08758-8)

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