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Validation of differential expression of microRNAs in exosomes from peripheral blood from pregnant women with preterm labor and preterm premature rupture of membranes and evaluation of regulatory mechanisms.

Grant number: 16/16618-1
Support Opportunities:Regular Research Grants
Duration: December 01, 2016 - August 31, 2019
Field of knowledge:Health Sciences - Medicine - Maternal and Child Health
Principal Investigator:Márcia Guimarães da Silva
Grantee:Márcia Guimarães da Silva
Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated researchers:Bruna Ribeiro de Andrade Ramos

Abstract

Preterm Labor (PTL) and Preterm Premature Rupture of Membranes (PPROM) are responsible for severe perinatal morbidity and neonatal mortality worldwide. MicroRNAs (miRNAs) can affect gestational outcome and are potencial biomarkers for these complications. Exosomes act in cell comunication, contain miRNAs and increasetheir stability. Objective: To compare the expression, in exosomes from peripheral blood, of the miRNAs miR-15-5p, miR-143-3p, miR-145-5p, miR-199a-5p, miR-200c-3p, miR-223-3p, miR338-3p and miR-493-3p between women with term pregnancies and PTL or PPROM, and to elucidate possible mechanisms of action of these miRNAs in the physiopathology of these adverse outcomes. Patients and methods: Case-control study with women that underwent PTL, PPROM, term pregnancy with and without labor and non-pregnant women seen at Botucatu Medical School. Exosomes will be isolated from plasma and expression of miRNAs will be performed byReal Time PCR. Later, miR-mimics or anti-miRs of miRNAs differentially expressed will be used to challenge cultures of chorioamniotic membranes. Pellets and supernatants from the cultures will be used to evaluate gene (pellets only) and proteinexpression of predicted targets of miRNAs. Levels of the cytokines IL-1², IL-6 and IL-8 will be measured in the supernatants. Statistical analysis will be performed according to the results obtained and a p-value of 5% will be adopted. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TRONCO, JULIA A.; RAMOS, BRUNA R. DE A.; BASTOS, NATALIA M.; ALCANTARA, SERGIO A.; DA SILVEIRA, JULIANO C.; DA SILVA, MARCIA G.. Alpha-2-macroglobulin from circulating exosome-like vesicles is increased in women with preterm pregnancies. SCIENTIFIC REPORTS, v. 10, n. 1, . (19/17279-4, 16/16618-1)
RAMOS, BRUNA RIBEIRO ANDRADE; TRONCO, JULIA ABBADE; CARVALHO, MARCIO; FELIX, TAINARA FRANCINI; REIS, PATRICIA PINTOR; SILVEIRA, JULIANO COELHO; SILVA, MARCIA GUIMARAES. Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 6, p. 13-pg., . (16/16618-1, 15/21829-9, 16/01340-8)

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