| Grant number: | 15/25638-3 |
| Support type: | Regular Research Grants |
| Duration: | November 01, 2016 - April 30, 2019 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Reproduction |
| Principal researcher: | José Antonio Dell'Aqua Junior |
| Grantee: | José Antonio Dell'Aqua Junior |
| Home Institution: | Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil |
| Assoc. researchers: | Fabiana Ferreira de Souza ; Fabio Morato Monteiro ; Fernanda da Cruz Landim ; Frederico Ozanam Papa ; João Pessoa Araújo Junior ; Mark Baker |
Abstract
Sperm sexing to obtain semen doses with a high percentage of gamete carriers X or Y chromosome, even more relevant the role of artificial insemination in maximizing genetic progress between generations; productivity and economic return in some farming activities; assisted reproduction in mammals; and genetic counseling of the human species. The objectives of this project will be study the sperm content X or Y chromosomes of cattle to select sex-specific proteins; identifying amino acid residues of interest proteins by mass spectrometry; produce sex-specific antibodies by heterologous immunization (chickens); and verifying the binding efficiency of these antibodies to bovine sperm. Differentials proteins encountered by mass spectrometry will be purified by liquid chromatography and tested for binding efficiency at 50% of cells of a non-sexed semen using flow cytometry, which checks the connection of the anti-protein antibody of interest. (AU)
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