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Biotechnological advances in sperm processing

Abstract

Sperm sexing to obtain semen doses with a high percentage of gamete carriers X or Y chromosome, even more relevant the role of artificial insemination in maximizing genetic progress between generations; productivity and economic return in some farming activities; assisted reproduction in mammals; and genetic counseling of the human species. The objectives of this project will be study the sperm content X or Y chromosomes of cattle to select sex-specific proteins; identifying amino acid residues of interest proteins by mass spectrometry; produce sex-specific antibodies by heterologous immunization (chickens); and verifying the binding efficiency of these antibodies to bovine sperm. Differentials proteins encountered by mass spectrometry will be purified by liquid chromatography and tested for binding efficiency at 50% of cells of a non-sexed semen using flow cytometry, which checks the connection of the anti-protein antibody of interest. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NOVELLO, GUILHERME; SEGABINAZZI, LORENZO G. T. M.; LISBOA, FERNANDO P.; CANUTO, LUCAS E.; FREITAS-DELL'AQUA, CAMILLA P.; DELL'AQUA JR, JOSE A.; CANISSO, IGOR F.. High or Low Body Fat Deposition in the Presence of a Normal Oral Sugar Test is Not Associated With Postthaw Semen Parameters in Stallions. Journal of Equine Veterinary Science, v. 95, . (15/25638-3)
SCOTT, CAROLINE; DE SOUZA, FABIANA F.; ARISTIZABAL, VIVIANA H. V.; HETHRINGTON, LOUISE; KRISP, CHRISTOPH; MOLLOY, MARK; BAKER, MARK A.; DELL'AQUA JUNIOR, JOSE ANTONIO. Proteomic profile of sex-sorted bull sperm evaluated by SWATH-MS analysis. Animal Reproduction Science, v. 198, p. 121-128, . (13/23351-3, 15/25638-3, 15/25698-6)
CARNEIRO, JOAO A. M.; CANISSO, IGOR F.; BANDEIRA, R. S.; SCHEEREN, V. F. C.; FREITAS-DELL'AQUA, CAMILA P.; ALVARENGA, MARCO A.; PAPA, FREDERICO O.; DELL'AQUA, JR., JOSE A.. Effects of coenzyme Q10 on semen cryopreservation of stallions classified as having good or bad semen freezing ability. Animal Reproduction Science, v. 192, p. 107-118, . (15/25638-3)

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