Multi-User Equipment approved in grant 15/50040-4: micro confocal high-content imaging system

Abstract

High-content screening (HCS), also known as high-content analysis (HCA) or cellomics, is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or RNAi that alter the phenotype of a cell in a desired manner. Hence high content screening is a type of phenotypic screen conducted in cells. Phenotypic changes may include increases or decreases in the production of cellular products such as proteins and/or changes in the morphology (visual appearance) of the cell. High content screening includes any method used to analyze whole cells or components of cells with simultaneous readout of several parameters. Hence the name "high content screening". Unlike high-content analysis, high-content screening implies a level of throughput which is why the term "screening" differentiates HCS from HCA, which may be high in content but low in throughput. The ImageXpress® Micro Confocal High-content Imaging System is a unique, flexible system for both widefield and confocal imaging of live or fixed samples. Proprietary AgileOptix" technology provides increased sensitivity resulting in high-contrast and high uniformity of intensity and shape across a large field of view achieving near-theoretical Z resolution. Multiple spinning disc geometries enable users to match the speed and resolution to their biology. (AU)