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Determination of the humoral antigenic profile from chagasic patients using Trypanosoma cruzi genome shotgun phage display

Grant number: 16/14506-1
Support type:Regular Research Grants
Duration: September 01, 2016 - August 31, 2018
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal researcher:Walter Colli
Grantee:Walter Colli
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Assoc. researchers:Edecio Cunha Neto ; Ester Cerdeira Sabino ; Maria Julia Manso Alves ; Ricardo Jose Giordano


Chagas' disease is a chronic illness and an important health issue in Brazil, Latin America and, more recently, in the world due to the steady increase in migration to developed countries. It is estimated that 200,000 people will die due to Chagas' disease in the next five years. Since there is no vaccine and existing therapies rely mostly on old drugs with limited efficacy and toxic side effects, it is crucial to develop novel therapeutic and diagnostic alternatives for the disease. Cardiomyopathy is the main cause of death, which develops in about a third of infected individuals. The reason why the other two thirds remain asymptomatic is still unclear. Due to the apparent lack of parasites in the heart and the presence of auto-antibodies in the sera, it has been proposed that Chagas' disease could be an autoimmune disease. However, using more advanced techniques, it has been shown that these patients still have extremely low number of parasites in their heart, which lead to inflammatory reactions that contribute to disease progression. However, the molecular mechanisms of disease progression are not yet fully understood. To help understanding the importance of the immune system in Chagas' disease, in particular the humoral response, we will explore the immune repertoire of T. cruzi using combinatorial biology. Genome shotgun libraries will be utilized to map the humoral response of T. cruzi infected patients throughout the different stages of the disease. Our expectation is that this work will lead to the identification and validation of novel T. cruzi antigens, which might contribute to the development of novel diagnostic and therapeutic options for Chagas' disease patients. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MATTOS, ELICIANE C.; CANUTO, GISELE; MANCHOLA, NUBIA C.; MAGALHAES, RUBENS D. M.; CROZIER, THOMAS W. M.; LAMONT, DOUGLAS J.; TAVARES, MARINA F. M.; COLLI, WALTER; FERGUSON, MICHAEL A. J.; ALVES, MARIA JULIA M. Reprogramming of Trypanosoma cruzi metabolism triggered by parasite interaction with the host cell extracellular matrix. PLoS Neglected Tropical Diseases, v. 13, n. 2 FEB 2019. Web of Science Citations: 1.

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