Antioxidant activity evaluation of gallic acid for cosmetic formulation.

Abstract

In order to maintain healthier and younger-looking skin, thus reducing the effects of aging, increasingly strategies have been suggested. Among the phenolic compounds, gallic acid has emerged as a powerful antioxidant, acts as retarding or inhibiting oxidative effects of free radicals on the organic molecules. Recently, the cosmetic industry and researchers have sought to use in vitro experiments, replacing animal models for the development of efficacy and safety tests of cosmetic materials and cosmetic formulations. Different methods have been developed for the determination of antioxidant capacity of compounds both by chemical or biological tests. Chemical tests are faster and simpler to run. Among the large number of gallic acid evidence, it is intended to carry out the assessment of its antioxidant activity, in order to prove their properties in order to make contributions to the pharmaceutical and cosmetic areas, since research in quality control cosmetic products for active content identification and study of the physical and chemical characteristics are of fundamental importance to ensure the quality of the final product. The objective of this project is the evaluation of the antioxidant capacity of gallic acid by in vitro methods, using spectrophotometric reading methods. The evaluation of gallic acid antioxidant potential will be realized through various methods, such as: ability to capture on the Radical Anion Superoxide; Crocina Bleaching Assay; Capture of hypochlorous acid; capturing ability of H2O2; capturing ability of the DPPH*+; capturing ability over ABTS*+; EnzymaticMethod using Kit SOD Assay (Sigma Aldrich). As standards will be used the trolox reagent (vitamin E analogue) and ascorbic acid, known compounds with antioxidant action and the experiments will be performed in triplicate. (AU)