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The development of analytical procedures for Hg and as speciation in environmental and biological samples by HPLC-ICP-MS and sample preparation with microextraction by packed sorbent (MEPS)


The quantification of a chemical element in a given matrix, in its full form, no longer brings the most relevant information for a complete understanding of how this element will affect living organisms or the environment, since in addition to the toxicity, bioavailability and mobility of an element are directly related to different species / existing chemical forms of this element. It is therefore crucial to determine the species in which these elements are found and properly quantify them. The analyzes for this purpose are called speciation analysis or simply chemical speciation. Currently, liquid chromatography-mass spectrometry with plasma inductively coupled (LC-ICP-MS), has been the technique of choice for chemical speciation in various types of samples at concentrations in L-1 ng-ug L -1. However, despite the great advances in analytical instrumentation in recent years in the chemical speciation area using LC-ICP-MS system, a sample preparation step (with analyte pre-concentration) are often still necessary, mainly due to the low concentration of the analytes in the sample and the presence of interfering in the matrix, aiming at the simplification and miniaturization of procedures, reducing the use of solvents and waste generated. Within this context, conventional techniques such as solid phase extraction (SPE) and liquid-liquid extraction (LLE) have been modified to eliminate the disadvantages associated with them. Among them, the solid phase microextraction (SPME) and microextraction packed sorbent (MEPS) are the two most successful miniaturized formats, with increasing number of applications in several areas, but with no application in chemical speciation.Thus, this project aims to evaluate new methods to differentiate between chemical species of arsenic and mercury in environmental samples (water) and clinical (plasma) using liquid chromatography hyphenated techniques and mass spectrometry plasma inductively coupled (LC -ICP-MS), and sample preparation by MEPS. As a result improvements are expected in various analytical characteristics compared to existing procedures in the literature, with reduced time for sample preparation and volume of reagents, as well as improvements in sensitivity and selectivity of the methods. To validate the methods various reference materials will be analyzed.Finally, the new procedures will help the various research activities related to the toxicology of Hg and As carried out by the Toxicology of Metals Lab at the School of Pharmaceutical Scienes of Ribeirão Preto-USP. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OLIVEIRA SOUZA, MARILIA CRISTINA; ROCHA, BRUNO ALVES; OLIVEIRA SOUZA, JULIANA MARIA; BERRETTA, ANDRESA APARECIDA; BARBOSA, JR., FERNANDO. A Fast and Simple Procedure for Polybrominated Diphenyl Ether Determination in Egg Samples by Using Microextraction by Packed Sorbent and Gas Chromatography-Mass Spectrometry. FOOD ANALYTICAL METHODS, v. 12, n. 7, p. 1528-1535, . (15/19665-8, 16/02949-6)
BRUNO ALVES ROCHA; MARIANA ZUCCHERATO BOCATO; ELDER FRANCISCO LATORRACA; JOÃO PAULO BIANCHI XIMENEZ; FERNANDO BARBOSA JR.. A survey of parabens in commercial baby wipes from Brazil and estimation of daily exposure. Química Nova, v. 43, n. 4, p. 442-446, . (16/02949-6, 14/50867-3, 13/23710-3, 14/19914-5)
DE OLIVEIRA, MARIANA LEPRI; ROCHA, BRUNO ALVES; DE OLIVEIRA SOUZA, VANESSA CRISTINA; BARBOSA, JR., FERNANDO. Determination of 17 potential endocrine-disrupting chemicals in human saliva by dispersive liquid-liquid microextraction and liquid chromatography-tandem mass spectrometry. Talanta, v. 196, p. 271-276, . (14/50867-3, 13/23710-3, 14/19914-5, 16/02949-6)

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