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Effects of sleep deprivation on the development of metastasis in a murine melanoma model


Sleep is hypothesized to play a restorative role on immune system. In addition, sleep deprivation (SP) and disturbed sleep is thought to impair host defense mechanisms. SP is considered a stressor, inasmuch as induces increased concentrations of glucocorticoids through activation of the hypothalamic-pituitary-adrenal (HPA) which can lead to a host of disorders resulting from prolonged secretion and / or increased these hormones. Given that stress is associated with reduced functioning of cytotoxic T cells and NK cells, processes such as immune surveillance against tumors mechanisms may be affected. Thus, the persistent activation of the HPA axis in response to stress, may contribute to the development and progression of tumors. Thus, this study aims to investigate the effects of PS on the development and tumor progression in a model of murine melanoma. The project is divided into four lines of research that focus more closely: changes in immune populations, both in the spleen as the infiltrative tumor, the formation of metastases and populations of immune spleen and infiltrating tumor microenvironment; production tissue and plasma cytokines and determination of plasma GC. For this, male mice of the C57BL/6 strain, will receive inoculation strain of murine melanoma B16F10 and will be submitted to the 18h of sleep restriction protocol lasting 21 days. The project is based on the expectation that a more detailed analysis of the various elements of the immune response against murine melanoma in response to sleep deprivation can result in both better monitoring of tumor development as the common understanding of the mechanisms of sleep and the immune system. (AU)

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