Effect topical insulin of the inflammataory and proliferetiva phase on 2nd degree-burns in diabetic-rats

Abstract

Burns are wounds caused for exposure to an agent of thermal, electric, radioactive orchemistry origin that presents attenuated inflammatory response, becoming even morecompromised when associated to diabetes mellitus. Despite the fact of experimentalevidences demonstrating tissue healing and reconstruction acceleration due to topical insulinuse in diabetic and control rats with incision wounds, there are no information regardinginsulin effect upon burn wounds. Our aim was to investigate the effect of topical insulincream on wound healing following 2nd-degree burns in diabetic rats. We induced seconddegreecutaneous burn wound in streptozotocin-induced diabetic rats and control rats with a1.0-cm diameter circular mold, 120°C, during 20 seconds. Burned rats received eitherplacebo cream or cream enriched with insulin (PI 0705370-3), once a day. At 7th and 14thdays after the wound induction, anesthetized animals had extraction of skin wound sites forElisa, immunohistochemistry, imunoblotting analysis and Weigert e Verhoeff staining. Theresults will be analyzed using the GraphPad Prism version 6.01® (GraphPad Software, LaJolla, CA, USA). The sample size per group analyzed for each parameter will be set throughN sufficient to perform the analysis of the distribution of the samples by "D'Agostino andPearson omnibus normality test" recommended by the GraphPad Prism version 6.01®program. The samples will be evaluated for their normal distribution and subjected toanalysis of variance for one and two factors (oneway and 2way ANOVA). When the test differmaterially from will be followed by Tukey's post-test for more than two variances (p <0.05). The results are expressed as mean ± standard deviation (M ± SD) and subsequentlyrepresented in varying percentages compared to controls, which will be assigned the value of100% the conditions under which it applies. (AU)