Research Grants 15/10006-1 - Urologia, Ratos - BV FAPESP
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EVALUATION OF CRANBERRY DIET SUPPLEMENTATION EFFECTS ON INDUCED NEPHROLITHIASIS IN RATS.

Abstract

The prevalence of urolithiasis (UL) has increased worldwide, with recurrence rates of around 50% after 10 years. It is known that urine is influenced by ingested fluid, which may change the chemical physical composition of the urine and the formation of new calculations. Although cranberry juice is known to be beneficial in the prevention of urinary tract infections, their role in the prevention of UL remains controversial. The aim of this study is to evaluate the metabolic and histological effects of dietary supplementation with cranberry on the UL in an experimental model in rats with induced hyperoxaluria. A total of 40 Sprague-Dawley adult male rats will be randomly distributed into three groups: G1 (n=10) clinical control - without intervention; G2 (n=15) hyperoxaluria - animals will receive 0.5% ethylene glycol diluted in water and 0.5µM of D3vitamin dissolved in one ml of vegetable oil, administered by gavage once a day; and G3 (n=15) - induced hyperoxaluria associated with administration of 800 mg / kg of animal weight cranberry diluted in water by gavage once a day. After4 weeks, the animals will be euthanized for conducting metabolic studies (calcium, oxalate, uric acid, and creatinine in serum and citrate in 24-hour urine). The renal parenchyma will be submitted to histological and histomorphometric analysis using the Image J® and determination of antioxidant enzymes for assessment of oxidative stress. Quantification of calcium deposited in the renal parenchyma will be performed by atomic spectrophotometry by PIXE technique (Proton Induced X-Ray). (AU)

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VEICULO: TITULO (DATA)
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