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Use of tocopherol in the attempt to recover the parotid gland (CRL-7669) induced to inflammation and oxidative stress

Grant number: 15/05742-0
Support Opportunities:Regular Research Grants
Duration: September 01, 2015 - August 31, 2017
Field of knowledge:Biological Sciences - Morphology - Anatomy
Principal Investigator:Eduardo José Caldeira
Grantee:Eduardo José Caldeira
Host Institution: Faculdade de Medicina de Jundiaí (FMJ). Prefeitura Municipal de Jundiaí. Jundiaí , SP, Brazil


Currently 4.6 million people die around the world by the diabetes mellitus and its complications. Approximately 10% of cases are type 1, among these, 90% occur in childhood, demonstrating the epidemiological importance of this disease. Diabetes may affect different tissues, including salivary glands, which have similar characteristics of pancreas. Diabetes mellitus is mainly associated with inflammatory reactions and oxidative stress, enhanced mainly by the action of lymphocytes and macrophages present in tissues affected by the disease. These processes seem to be common in all injured tissues as a consequence of autoimmune effect and hyperglycemia that increase the production of reactive oxygen species (ROS). ROS can participate as a synergistic agent in this disease, and is a signalling of the cell apoptosis, protein oxidation, and lipid peroxidation, the latter plays an important role in the pathogenesis of this disease. On the other hand, the antioxidant activity of vitamin E can prevent these processes, especially those related to oxidative stress, it has a significant role in the protection of biological membranes, blocking damage caused by free radicals generated by this condition. Thus, the objective of this study is to investigate the antioxidant effects of tocopherol (vitamin E) in relation to inflammation and oxidative stress induced in CRL-7669 cells (parotid gland cell line). The cell lines will be cultured in Dulbecco's modified Eagle medium (DMEM). Subsequently, the cells will be induced to inflammation and oxidative stress. After, will be realized the treatment with the proposed antioxidant. Following the experimental period, the samples will be analyzed to observe the cellular evolution by cell culture procedures and immunoblotting, complemented by real time PCR for the quantitation of DNA and RNA fragments, and Dihydroetidio Reaction (EHD) to determine the active oxidizing agent (hydroxyl radical, OH) (AU)

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