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Dental pulp cell differentiation and biomineralization: cell culture assays, experimental models and clinical application in human


The advances in the development of materials for conservative pulp tissue therapy has allowed the preservation of the vitality of tissue and tissue healing through the mineralized neoformation. Biodentine" and MTA are dental materials used for this purpose, although the mechanisms invoved in biomineralization induced by these materials are not fully understood. Therefore, further studies should be conducted both in vitro as in vivo to shed light on this important biological repairing process through investigation of the molecules and signalling pathways involved. For this purpose, to dissect possible mechanisms of inflammation and repair, in vitro models of human dental pulp cell culture will be used and in vivo biompatibility will be investigated in the subcutaneous tissue of mice and in dogs teeth. In a second phase, we aim to evaluate, in a randomized clinical study, if the materials showing histological satisfactory results could be indicated for pulpotomy in humans primary teeth. Finally, in an animal model, we will evaluate whether the deposition of mineralized tissue induced by dental pulp cells could be mimicked by periodontal ligament cells to stimulate the repair after furcation perforation, which still represents a clinical situation difficult to be treated. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HUAINAN, STEPHANIE DIAZ; BRITO ARAGAO, MARIA GERUSA; DIAS MORENO, ANA PAULA; DE QUEIROZ, ALEXANDRA MUSSOLINO; BEZERRA SILVA, RAQUEL ASSED; GARCIA DE PAULA-SILVA, FRANCISCO WANDERLEY; BEZERRA SILVA, LEA ASSED. Accuracy of Conventional Periapical Radiography in Diagnosing Furcation Repair after Perforation Treatment. JOURNAL OF ENDODONTICS, v. 46, n. 6, p. 827-831, JUN 2020. Web of Science Citations: 0.
SILVA, R. A. B.; BORGES, A. T. N.; HERNANDEZ-GATON, P.; DE QUEIROZ, A. M.; ARZATE, H.; ROMUALDO, P. C.; NELSON-FILHO, P.; SILVA, L. A. B. Histopathological, histoenzymological, immunohistochemical and immunofluorescence analysis of tissue response to sealing materials after furcation perforation. International Endodontic Journal, v. 52, n. 10, p. 1489-1500, OCT 2019. Web of Science Citations: 0.
BEZERRA SILVA, LEA ASSED; MICHELAO GRECCA PIERONI, KARINA ALESSANDRA; NELSON-FILHO, PAULO; BEZERRA SILVA, RAQUEL ASSED; HERNANDEZ-GATON, PATRICIA; LUCISANO, MARILIA PACIFICO; GARCIA PAULA-SILVA, FRANCISCO WANDERLEY; DE QUEIROZ, ALEXANDRA MUSSOLINO. Furcation Perforation: Periradicular Tissue Response to Biodentine as a Repair Material by Histopathologic and Indirect lmmunofluorescence Analyses. JOURNAL OF ENDODONTICS, v. 43, n. 7, p. 1137-1142, JUL 2017. Web of Science Citations: 12.
DALTOE, MARIANA O.; PAULA-SILVA, FRANCISCO WANDERLEY G.; FACCIOLI, LUCIA H.; GATON-HERNANDEZ, PATRICIA M.; DE ROSSI, ANDIARA; BEZERRA SILVA, LEA ASSED. Expression of Mineralization Markers during Pulp Response to Biodentine and Mineral Trioxide Aggregate. JOURNAL OF ENDODONTICS, v. 42, n. 4, p. 596-603, APR 2016. Web of Science Citations: 20.

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