Novel nanobiomaterials based on tridimensional peptides with drug delivery properties to tissue engineering

Abstract

This FAPESP-MIT proposal is part of the Young Researcher FAPESP project "Development of Novel Polymeric Scaffolds by Electrospinning with Incorporated Vertically-Aligned Carbon Nanotubes and Nanohidroxyapatite for Bone Regeneration" (2011/17877-7). In particular, herein we propose to extend this work with the inclusion of polymers for the controlled release of therapeutic peptides and drugs. This new direction will significantly expand the utility of original work, and open it to new medical applications. it includes the development a new polymers based on peptides with drug delivery properties. Initially, the submitted proposal was based on polymer scaffold fabrication with the incorporation of nanoparticles by electrospinning technique for bone tissue regeneration. The principal investigator used superhydrophilic vertical aligned carbon nanotubes, dispersed carbon nanotubes, Ti02 and nanohydroxyapatite nanoparticles. We obtained roughness robust nanofibers using electrospinning and rotary-jet spinning techniques. Also, we produced nanofibers with incorporated nanoparticles up to 5% (w/w). This result was very attractive to obtained superhydrophilicity, cellular adhesion and calcification of human osteoblasts. However, we used synthetic and commercial polymers, which limited precluded it the development of new nano-biomaterials relevant to tissue engineering. to development new nanobiomaterials to tissue enginnering. The MIT Jonson'Johnsons group at MIT has developed new polymeric nano-architectures with applications suitable for peptide and drug delivery. Thus, in this proposal, we seek to leverage the expertise of both groups to develop next-generation fibers using our established electrospinning techniques and Johnson's polymeric nanoparticles. development new polymers based on peptides and with drug delivering properties. The proposed collaborative research Besides this, this collaboration process will be very interesting to both research's groups, because the Laboratory of Biomedical Nanotechnology coordinated by this proponent have experience in extrude polymerr extrusions using electrospinning and rotary-jet spinning, which will augment Johnson's current research program. Also, the undergraduate and Ph.D students will perform biological in vitro and in vivo assays, which willlead to rapid translation of our novel materials. This project presents an innovative technology for manufacturing nanofibers scaffolds. We expected expect to develop a new three-tridimensional nanobiomaterial with biomimetic properties that improve the bone tissue regeneration and reduced the inflammation process. Thus, following the methodology of this project it is expected that this new nanomaterial can: accelerate the regeneration of bone tissue, recruit stem cells, reduce the risk bacterial contamination and possess low cost be sufficiently low-cost to be translated into clinical to society making them candidates for new nanobiomaterials for be used in regenerative medicine applications. Also, we expect to installed a new research line program at University of Vale do Paraiba in São Paulo state, which will produceing new biomimetic materiais based on the polymersthree-dimensional peptide-based fibers developed herein. tridimensional construct based on peptides with drug delivery properties. Also astly, we pretend will involve chemistry, chemical engineering, and biomedical engineering undergraduated and Ph.D students throughout the proposed research. This research field is better explored in Brazil and this collaboration will be strengthening this. The proposed research will augment the already well-established field of tissue engineering in Brazil through constructive interactions with MIT. (AU)