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Sugar cane biomass recalcitrance: basic knowledge related to the cell wall construction, pretreatment and enzymatic digestion, applied for the development of innovative biorefinery models

Abstract

The project will provide integrated knowledge related to the sugar cane cell wall recalcitrance. Based on this knowledge, innovative biorefinery models will be proposed for the conversion of the sugar cane biomass into valuable products. Secondary cell wall formation will be evaluated along complete maturation cycles experimental hybrids that contrast for lignocellulose composition. UV- and fluorescence-microscopy will be used to evaluate the lignification and the hemicellulose deposition processes, respectively. Expression of monolignols biosynthesis genes will be evaluated as well. Studied samples will be evaluated also according to their digestibility by hydrolytic enzymes. Data compilation will give a general view of the cell wall formation in sugar cane and how deposition of the cellulose-encapsulating agents (lignin, hydroxicinnamic acids and hemicellulose) affects the biomass recalcitrance. New biorefinery models for sugar cane biomass conversion will be designed with basis on the accumulated knowledge. It is advanced that mild pretreatment processes based on the action of alkaline-sulfite or diluted acids will provide substrates susceptible for xylanase pre-digestion that could provide valuable hemicellulose olygosaccharides. After this step, the remaining substrate would be susceptible for efficient cellulose hydrolysis by cellulases, producing fermentable sugars. New hydrolytic enzymes identified with basis on the secretome study of wood decay fungi will be tested for the hydrolysis of specific pretreated materials. A final evaluation of the designed processes with basis on life cycle assessment will indicate which benefits could be obtained by the use of low-recalcitrance plants pretreated by specially designed pretreatment steps (AU)

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Scientific publications (34)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CORNETTI, ALINE A. A.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Enzyme-aided xylan extraction from alkaline-sulfite pretreated sugarcane bagasse and its incorporation onto eucalyptus kraft pulps. Carbohydrate Research, v. 492, . (14/06923-6)
SARTORI, GUILHERME P.; VASCONCELOS, MARCELO H.; SANTOS, JULIO C.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE. An innovative concept for industrial sugarcane processing enhances polysaccharide utilization in first- and second-generation integrated biorefineries. INDUSTRIAL CROPS AND PRODUCTS, v. 141, . (15/23684-8, 14/06923-6)
VALADARES, FERNANDA; GONCALVES, THIAGO A.; DAMASIO, ANDRE; MILAGRES, ADRIANE M. F.; SQUINA, FABIO M.; SEGATO, FERNANDO; FERRAZ, ANDRE. The secretome of two representative lignocellulose-decay basidiomycetes growing on sugarcane bagasse solid-state cultures. Enzyme and Microbial Technology, v. 130, . (17/16089-1, 15/50590-4, 14/06923-6)
VELASCO, JOSMAN; PELLEGRINI, VANESSA DE OLIVEIRA ARNOLDI; SEPULCHRO, ANA GABRIELA VEIGA; KADOWAKI, MARCO ANTONIO SEIKI; SANTO, MELISSA CRISTINA ESPIRITO; POLIKARPOV, IGOR; SEGATO, FERNANDO. Comparative analysis of two recombinant LPMOs from Aspergillus fumigatus and their effects on sugarcane bagasse saccharification. Enzyme and Microbial Technology, v. 144, . (19/22284-7, 15/13684-0, 14/06923-6, 14/18714-2, 19/01165-0, 17/50025-0)
SPORCK, DANIELE; REINOSO, FELIPE A. M.; RENCORET, JORGE; GUTIERREZ, ANA; DEL RIO, JOSE C.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Xylan extraction from pretreated sugarcane bagasse using alkaline and enzymatic approaches. BIOTECHNOLOGY FOR BIOFUELS, v. 10, . (14/06923-6, 08/56256-5)
MAMEDES-RODRIGUES, T. C.; BATISTA, D. S.; NAPOLEAO, T. A.; CRUZ, A. C. F.; FORTINI, E. A.; NOGUEIRA, F. T. S.; ROMANEL, E.; OTONI, W. C.. Lignin and cellulose synthesis and antioxidative defense mechanisms are affected by light quality in Brachypodium distachyon. PLANT CELL TISSUE AND ORGAN CULTURE, v. 133, n. 1, p. 1-14, . (14/06923-6, 16/24391-7, 14/17486-6)
DE VRIES, RONALD P.; RILEY, ROBERT; WIEBENGA, AD; AGUILAR-OSORIO, GUILLERMO; AMILLIS, SOTIRIS; UCHIMA, CRISTIANE AKEMI; ANDERLUH, GREGOR; ASADOLLAHI, MOJTABA; ASKIN, MARION; BARRY, KERRIE; et al. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus. Genome Biology, v. 18, . (14/10351-8, 12/19040-0, 14/06923-6, 11/08945-9, 14/11766-7, 12/20549-4)
REINOSO, FELIPE A. M.; RENCORET, JORGE; GUTIERREZ, ANA; MILAGRES, ADRIANE M. F.; DEL RIO, JOSE C.; FERRAZ, ANDRE. Fate of p-hydroxycinnamates and structural characteristics of residual hemicelluloses and lignin during alkaline-sulfite chemithermomechanical pretreatment of sugarcane bagasse. BIOTECHNOLOGY FOR BIOFUELS, v. 11, . (14/06923-6)
DE OLIVEIRA SANTOS, VICTOR TABOSA; SIQUEIRA, GERMANO; FERREIRA MILAGRES, ADRIANE MARIA; FERRAZ, ANDRE. Role of hemicellulose removal during dilute acid pretreatment on the cellulose accessibility and enzymatic hydrolysis of compositionally diverse sugarcane hybrids. INDUSTRIAL CROPS AND PRODUCTS, v. 111, p. 722-730, . (14/06923-6)
COSTA, THALES H. F.; VEGA-SANCHEZ, MIGUEL E.; MILAGRES, ADRIANE M. F.; SCHELLER, HENRIK V.; FERRAZ, ANDRE. Tissue-specific distribution of hemicelluloses in six different sugarcane hybrids as related to cell wall recalcitrance. BIOTECHNOLOGY FOR BIOFUELS, v. 9, . (14/06923-6, 08/56256-5, 11/50535-2)
SIQUEIRA, GERMANO; ARANTES, VALDEIR; SADDLER, JACK N.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Limitation of cellulose accessibility and unproductive binding of cellulases by pretreated sugarcane bagasse lignin. BIOTECHNOLOGY FOR BIOFUELS, v. 10, . (14/06923-6, 08/56256-5, 12/09940-3)
MESQUITA, JESSICA FARIA; FERRAZ, ANDRE; AGUIAR, ANDRE. Alkaline-sulfite pretreatment and use of surfactants during enzymatic hydrolysis to enhance ethanol production from sugarcane bagasse. Bioprocess and Biosystems Engineering, v. 39, n. 3, p. 441-448, . (14/06923-6, 08/56256-5)
MENDES, F. M.; DIAS, M. O. S.; FERRAZ, A.; MILAGRES, A. M. F.; SANTOS, J. C.; BONOMI, A.. Techno-economic impacts of varied compositional profiles of sugarcane experimental hybrids on a biorefinery producing sugar, ethanol and electricity. CHEMICAL ENGINEERING RESEARCH & DESIGN, v. 125, p. 72-78, . (14/06923-6, 15/10756-0)
VALADARES, FERNANDA; GONCALVES, THIAGO A.; GONCALVES, DAYELLE S. P. O.; SEGATO, FERNANDO; ROMANEL, ELISSON; MILAGRES, ADRIANE M. F.; SQUINA, FABIO M.; FERRAZ, ANDRE. Exploring glycoside hydrolases and accessory proteins from wood decay fungi to enhance sugarcane bagasse saccharification. BIOTECHNOLOGY FOR BIOFUELS, v. 9, . (14/06923-6, 08/56256-5)
CESARINO, IGOR; SIMOES, MARCELLA SIQUEIRA; BRITO, MICHAEL DOS SANTOS; FANELLI, AMANDA; SILVA, TATIANE DA FRANCA; ROMANEL, ELISSON. Building the wall: recent advances in understanding lignin metabolism in grasses. ACTA PHYSIOLOGIAE PLANTARUM, v. 38, n. 11, . (14/08468-4, 15/18361-5, 14/06923-6, 14/23017-9, 15/02527-1, 14/17486-6)
RUBIO, MARCELO VENTURA; ZUBIETA, MARIANE PALUDETTI; LOURENO FRANCO CAIRO, JOAO PAULO; CALZADO, FELIPE; PAES LEME, ADRIANA FRANCO; SQUINA, FABIO MARCIO; PRADE, ROLF ALEXANDER; DE LIMA DAMASIO, ANDRE RICARDO. Mapping N-linked glycosylation of carbohydrate-active enzymes in the secretome of Aspergillus nidulans grown on lignocellulose. BIOTECHNOLOGY FOR BIOFUELS, v. 9, . (14/15403-6, 14/06923-6, 11/20977-3, 13/24988-5, 12/20549-4, 14/23051-2, 09/54067-3)
MACHADO, ANGELA DA SILVA; FERRAZ, ANDRE. Biological pretreatment of sugarcane bagasse with basidiomycetes producing varied patterns of biodegradation. Bioresource Technology, v. 225, p. 17-22, . (14/06923-6, 08/56256-5)
MACHADO, ANGELA S.; VALADARES, FERNANDA; SILVA, TATIANE F.; MILAGRES, ADRIANE M. F.; SEGATO, FERNANDO; FERRAZ, ANDRE. The Secretome ofPhanerochaete chrysosporiumandTrametes versicolorGrown in Microcrystalline Cellulose and Use of the Enzymes for Hydrolysis of Lignocellulosic Materials. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 8, . (14/18714-2, 14/06923-6)
VELASCO, JOSMAN; OLIVA, BIANCA; GONCALVES, ALINE LARISSA; LIMA, AWANA SILVA; FERREIRA, GISLENE; FRANCA, BRUNO ALVES; MULINARI, EVANDRO JOSE; GONCALVES, THIAGO AUGUSTO; SQUINA, FABIO MARCIO; SEIKI KADOWAKI, MARCO ANTONIO; et al. Functional characterization of a novel thermophilic exo-arabinanase fromThermothielavioides terrestris. Applied Microbiology and Biotechnology, v. 104, n. 19, . (14/18714-2, 17/16089-1, 14/06923-6, 19/01165-0, 15/50590-4, 19/06663-8)
RAMOS, LUCAS; VASCONCELOS, MARCELO H.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE; DIAS, MARINA O. S.; MENDES, FERNANDA M.; DOS SANTOS, JULIO C.. High-solid enzymatic hydrolysis of sugarcane bagasse and ethanol production in repeated batch process using column reactors. 3 BIOTECH, v. 11, n. 10, . (15/10756-0, 14/06923-6)
COLLUCCI, DANIEL; BUENO, RAPHAEL C. A.; MILAGRES, ADRIANE M. F.; FERRAZ, ANDRE. Sucrose content, lignocellulose accumulation and in vitro digestibility of sugarcane internodes depicted in relation to internode maturation stage and Saccharum genotypes. INDUSTRIAL CROPS AND PRODUCTS, v. 139, . (16/02985-2, 14/06923-6)
VASCONCELOS, MARCELO HOLANDA; MENDES, FERNANDA MACHADO; RAMOS, LUCAS; DIAS, MARINA OLIVEIRA S.; BONOMI, ANTONIO; JESUS, CHARLES DAYAN F.; WATANABE, MARCOS DJUN B.; JUNQUEIRA, TASSIA LOPES; MILAGRES, ADRIANE MARIA F.; FERRAZ, ANDRE; et al. Techno-economic assessment of bioenergy and biofuel production in integrated sugarcane biorefinery: Identification of technological bottlenecks and economic feasibility of dilute acid pretreatment. ENERGY, v. 199, . (15/10756-0, 14/06923-6)
BERTO, GABRIELA LEILA; VELASCO, JOSMAN; CABOS RIBEIRO, CAIO TASSO; ZANPHORLIN, LETICIA MARIA; DOMINGUES, MARIANE NORONHA; MURAKAMI, MARIO TYAGO; POLIKARPOV, IGOR; DE OLIVEIRA, LEANDRO CRISTANTE; FERRAZ, ANDRE; SEGATO, FERNANDO. Functional characterization and comparative analysis of two heterologous endoglucanases from diverging subfamilies of glycosyl hydrolase family 45. Enzyme and Microbial Technology, v. 120, p. 23-35, . (14/06923-6, 15/13684-0, 14/18714-2, 15/26982-0)
MONTE, JOSEANA R.; LAURITO-FRIEND, DEBORA F.; MUSSATTO, I, SOLANGE; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Comparative Evaluation of Acid and Alkaline Sulfite Pretreatments for Enzymatic Saccharification of Bagasses from Three Different Sugarcane Hybrids. BIOTECHNOLOGY PROGRESS, v. 34, n. 4, p. 944-951, . (14/06923-6)
PIMENTEL, AGNES C.; EMATSU, GABRIELA C. G.; LIBERATO, MARCELO V.; PAIXAO, DOUGLAS A. A.; FRANCO CAIRO, JOAO PAULO L.; MANDELLI, FERNANDA; TRAMONTINA, ROBSON; GANDIN, CESAR A.; DE OLIVEIRA NETO, MARIO; SQUINA, FABIO M.; et al. Biochemical and biophysical properties of a metagenome-derived GH5 endoglucanase displaying an unconventional domain architecture. International Journal of Biological Macromolecules, v. 99, p. 384-393, . (16/01926-2, 14/06923-6, 10/11469-1, 14/12861-3, 14/04105-4, 08/58037-9)
CALZADO, FELIPE; PRATES, ERICA T.; GONCALVES, THIAGO A.; RUBIO, MARCELO V.; ZUBIETA, MARIANE P.; SQUINA, FABIO M.; SKAF, MUNIR S.; DAMASIO, ANDRE R. L.. Molecular basis of substrate recognition and specificity revealed in family 12 glycoside hydrolases. Biotechnology and Bioengineering, v. 113, n. 12, p. 2577-2586, . (14/06923-6, 12/20549-4, 13/08293-7, 14/23051-2, 13/24988-5)
BATISTA, DIEGO SILVA; KOEHLER, ANDREA DIAS; ROMANEL, ELISSON; DE SOUZA, VINICIUS CAIRUS; SILVA, TATIANE DULCINEIA; ALMEIDA, MAIRA CAROLINA; MACIEL, TALLES ELISSON F.; BUENO FERREIRA, PERACIO RAFAEL; SOUSA FELIPE, SERGIO HEITOR; SALDANHA, CLEBER WITT; et al. De novo assembly and transcriptome of Pfaffia glomerata uncovers the role of photoautotrophy and the P450 family genes in 20-hydroxyecdysone production. PROTOPLASMA, v. 256, n. 3, p. 601-614, . (14/06923-6, 14/17486-6)
HAHN SCHNEIDER, WILLIAN DANIEL; GONCALVES, THIAGO AUGUSTO; UCHIMA, CRISTIANE AKEMI; COUGER, MATTHEW BRIAN; PRADE, ROLF; SQUINA, FABIO MARCIO; PINHEIRO DILLON, ALDO JOSE; CAMASSOLA, MARLI. Penicillium echinulatum secretome analysis reveals the fungi potential for degradation of lignocellulosic biomass. BIOTECHNOLOGY FOR BIOFUELS, v. 9, . (14/06923-6, 12/19040-0, 08/58037-9)
MENDES, F. M.; VASCONCELOS, M. H.; DIAS, M. O. S.; FERRAZ, A.; MILAGRES, A. M. F.; SANTOS, J. C.; JESUS, C. D. F.; WATANABE, M. D. B.; JUNQUEIRA, T. L.; BONOMI, A.. Alkaline sulfite pretreatment for integrated first and second generation ethanol production: A techno-economic assessment of sugarcane hybrids. BIOMASS & BIOENERGY, v. 119, p. 314-321, . (15/10756-0, 14/06923-6)
MAINARDI, PEDRO H.; FEITOSA, VALKER A.; BRENELLI DE PAIVA, LIVIA B.; BONUGLI-SANTOS, RAFAELLA C.; SQUINA, FABIO M.; PESSOA, JR., ADALBERTO; SETTE, LARA D.. Laccase production in bioreactor scale under saline condition by the marine-derived basidiomycete Peniophora sp CBMAI 1063. FUNGAL BIOLOGY, v. 122, n. 5, p. 302-309, . (13/19486-0, 14/06923-6, 13/12505-0, 16/07957-7, 13/03061-0)
SANTOS, MAIARA P.; REINOSO, FELIPE A. M.; TAVILLA, VERONICA; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. On-site produced and commercially available alkali-active xylanases compared for xylan extraction from sugarcane bagasse. BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY, v. 18, . (14/06923-6)
MENDES, FERNANDA M.; FONSECA, MARIANA B.; FERRAZ, ANDRE; MILAGRES, ADRIANE M. F.. Anatomic and Ultrastructural Characteristics of Different Regions of Sugar Cane Internodes Which Affect Their Response to Alkaline Sulfite Pretreatment and Material Recalcitrance. ENERGY & FUELS, v. 30, n. 2, p. 1078-1084, . (14/06923-6, 08/56256-5, 11/50535-2)
FANELLI, AMANDA; REINHARDT, LAURIE; MATSUOKA, SIZUO; FERRAZ, ANDRE; SILVA, TATIANE DA FRANCA; HATFIELD, RONALD D.; ROMANEL, ELISSON. Biomass composition of two new energy cane cultivars compared with their ancestral Saccharum spontaneum during internode development. BIOMASS & BIOENERGY, v. 141, . (14/06923-6, 16/24391-7, 16/25785-9, 14/17486-6)

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Filed patent(s) as a result of this research project

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR1020180168185 - Universidade de São Paulo (USP) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - August 2018, 16