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Research of modulating the anti-inflammatory protein Annexin A1 in different cancer cells of the cervix

Abstract

The cervical cancer is the second type of more frequent cancer in women world-wide, being the fourth cause of death for cancer in developing countries. Cervical carcinogenesis is related with genetic alterations, infection for Human PapilomaVirus (HPV), angiogenesis and inflammatory processes. The idea of that the inflammation is involved in tumorigenesis is supported by the comment of that the cancer appears frequently in areas of chronic inflammation. On the other hand, the inflammatory reply is controlled for the action of anti-inflammatory mediators, who act to keep the homeostasis of the immunological reply to prevent the tecidual injury. Among these mediators we detach the annexin A1 (ANXA1), protein of 37 kDa, that is express for the tumoral cells and acts as modulating of the inflammatory process. However, the molecular mechanisms for which the ANXA1 modulates the cellular answers in the inflammatory processes are not yet completely determined. The available data suggest that this protein family can have, beyond its important role in the inflammatory process, a significant involvement in the cancer, through signaling cascades that include genes related with the cellular cycle, the differentiation and apoptosis. Ahead of these considerations, we will investigate, in vitro, the influence of this anti-inflammatory in the SiHa, HeLa and HaCaT cells on the morphology, cellular proliferation and migration, cytokines/chemokines quantification and in the proteic and EP3, EP4, MMP2 e MMP9 gene expression, observing as the ANXA1 modulates the expression of these genes and as these alterations can participate of the tumorigenic process. For this, the cell line SiHa, HeLa and HaCaT (derived from the cervical squamous cell carcinoma, cervical cell adenocarcinoma and human keratnocytes normal cells, respectively) will be used, treated with ANXA1 for 2, 4, 24, 48, 72 and 120 hours. Specifically, it will be investigated by molecular and cellular approaches how this anti-inflammatory play in specific genetic way, in the cytokine way, and may research the potential marker's expression related with tumorigenics process in cervix carcinoma. Then, we can get possible molecular markers to direct individual's genetics therapy. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARDIN, LAILA TONIOL; SONEHARA, NATHALIA MARTINS; OLIVEIRA MIMURA, KALLYNE KIOKO; DINARTE DOS SANTOS, ANEMARI RAMOS; DA SILVA JUNIOR, WILSON ARAUJO; SOBRAL, LAYS MARTIN; LEOPOLDINO, ANDREIA MACHADO; DA CUNHA, BIANCA RODRIGUES; TAJARA, ELOIZA H.; OLIANI, SONIA MARIA; et al. ANXA1(Ac2-26) peptide, a possible therapeutic approach in inflammatory ocular diseases. Gene, v. 614, p. 26-36, . (12/08320-1, 16/02012-4, 13/24083-2)
CARDIN, LAILA TONIOL; PRATES, JANESLY; DA CUNHA, BIANCA RODRIGUES; TAJARA, ELOIZA HELENA; OLIANI, SONIA MARIA; RODRIGUES-LISONI, FLAVIA CRISTINA. Annexin A1 peptide and endothelial cell-conditioned medium modulate cervical tumorigenesis. FEBS OPEN BIO, v. 9, n. 4, p. 668-681, . (16/02012-4, 13/24083-2)
FRANCO-SALLA, GABRIELA BUENO; PRATES, JANESLY; CARDIN, LAILA TONIOL; DINARTE DOS SANTOS, ANEMARI RAMOS; DA SILVA, JR., WILSON ARAUJO; DA CUNHA, BIANCA RODRIGUES; TAJARA, ELOIZA HELENA; OLIANI, SONIA MARIA; RODRIGUES-LISONI, FLAVIA CRISTINA. Euphorbia tirucalli modulates gene expression in larynx squamous cell carcinoma. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v. 16, . (13/24083-2, 12/07853-6)

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