Advanced search
Start date

Effects of enzymatic removal of plant cell wall acylation (acetylation, p-coumaroylation and feruloylation) on accessibility of cellulose and xylan in natural (non-pretreated) sugar cane fractions

Grant number: 14/21158-4
Support Opportunities:Regular Research Grants - Publications - Scientific article
Duration: November 01, 2014 - April 30, 2015
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Principal Investigator:Andre Luis Ferraz
Grantee:Andre Luis Ferraz
Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil


The central part of the sugar cane internodes (pith) was used as a substrate to evaluate sugar cane digestibility by acessory enzymes combined with cellulases and xylanases. Acetyl xylan esterase (TrAXE) was more efficient in enhancing the overall saccharification of sugar cane as compared to the feruloyl esterases AnFaeA, TsFaeC and NcFaeD. The hydroxycinnamic acid composition of sugar cane fractions and the hydrolysis data together suggest that feruloyl groups are more likely to decorate xylan, while p-coumaroyl groups are rather linked to lignin. The three different feruloyl esterases had distinct product profile on non-pretreated sugar cane substrate, indicating that sugar cane pith could function as a possible natural substrate for feruloyl esterase activity measurements. Hydrolysis data suggest TsFaeC was able to release p-coumaroyl groups esterifying lignin. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items

Please report errors in scientific publications list using this form.