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Salmonella enterica: roles of NAPS in pathogenicity and virulence

Grant number: 14/13412-8
Support Opportunities:Regular Research Grants
Duration: October 01, 2014 - March 31, 2017
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Marcelo Brocchi
Grantee:Marcelo Brocchi
Host Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Recent results from our group indicated that mutants of Salmonella enterica for genes encoding NAPs (nucleoid-associated proteins) are virulence attenuated and able to induce, in different extension, protection in the mouse model of infection. These results suggest an important role of NAPs in bacterial virulence. Based on this, the aim of this project is further study the role of different NAPs in the biology of S. enterica Typhimurium and Enteritidis. New mutants will be constructed and phenotypically evaluated with regard growth in vitro, acid-resistance, bile salts, invasion, persistence and multiplication in macrophages and biofilm formation. Virulence attenuation and immunogenicity will be evaluated in the murine model of infection. Also, high-throughput methodologies will be employed to study gene expression. The results will bring new information on the role of NAPs in S. enterica virulence. In addition, attenuated mutants of S. enterica will be evaluated regarding the therapeutic effect on tumors in the mouse model. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MILANEZ, GUILHERME PAIER; NASCIMENTO, LEANDRO COSTA; TIRABASSI, ADRIANE HOLTZ; ZUANAZE, MARCELO; RODRIGUES, DALIA PRAZERES; GUIMARAES PEREIRA, GONCALO AMARANTE; BROCCHI, MARCELO. Whole-Genome Sequence of Salmonella enterica Serovar Enteritidis Phage Type 4, Isolated from a Brazilian Poultry Farm. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 4, n. 3, . (14/13412-8, 12/05382-6)
CALARGA, ALINE PAROLIN; PARDINI GONTIJO, MARCO TULIO; PAULA DE ALMEIDA, LUIZ GONZAGA; RIBEIRO DE VASCONCELOS, ANA TEREZA; NASCIMENTO, LEANDRO COSTA; COTRIM DE MORAES BARBOSA, TAISE MARONGIO; DE CARVALHO PERRI, THALITA MARA; DOS SANTOS, SILVIA REGINA; TIBA-CASAS, MONIQUE RIBEIRO; LEMES MARQUES, ENEIDA GONCALVES; et al. Antimicrobial resistance and genetic background of non-typhoidal Salmonella enterica strains isolated from human infections in Sao Paulo, Brazil (2000-2019). Brazilian Journal of Microbiology, v. 53, n. 3, p. 14-pg., . (17/10051-2, 14/13412-8)
MILANEZ, GUILHERME PAIER; NASCIMENTO, LEANDRO COSTA; TIRABASSI, ADRIANE HOLTZ; ZUANAZE, MARCELO; RODRIGUES, DALIA PRAZERES; GUIMARAES PEREIRA, GONCALO AMARANTE; BROCCHI, MARCELO. Whole-Genome Sequence of Salmonella enterica Serovar Enteritidis Phage Type 4, Isolated from a Brazilian Poultry Farm. GENOME ANNOUNCEMENTS, v. 4, n. 3, p. 2-pg., . (14/13412-8, 12/05382-6)
WERLE, CATIERINE HIRSCH; DAMIANI, IGOR; MILANEZ, GUILHERME PAIER; FARIAS, ALESSANDRO S.; CINTRA GOMES MARCONDES, MARIA CRISTINA; CULLER, HEBERT FABRICIO; SIRCILI, MARCELO PALMA; LEITE, BRUNA; BROCCHI, MARCELO. Antimelanoma effect of Salmonella Typhimurium integration host factor mutant in murine model. FUTURE ONCOLOGY, v. 12, n. 20, p. 2367-2378, . (12/25426-8, 12/05382-6, 14/13412-8)
MILANEZ, GUILHERME P.; WERLE, CATIERINE H.; AMORIM, MARIENE R.; RIBEIRO, RAFAEL A.; TIBO, LUIZ H. S.; ROQUE-BARREIRA, MARIA CRISTINA; OLIVEIRA, ALINE F.; BROCCHI, MARCELO. HU-Lacking Mutants of Salmonella enterica Enteritidis Are Highly Attenuated and Can Induce Protection in Murine Model of Infection. FRONTIERS IN MICROBIOLOGY, v. 9, . (13/04088-0, 12/05382-6, 14/13412-8, 14/05359-0)
RAMPAZZO, P. E.; MARCOS, F. C. C.; CIPRIANO, M. A. P.; MARCHIORI, P. E. R.; FREITAS, S. S.; MACHADO, E. C.; NASCIMENTO, L. C.; BROCCHI, M.; RIBEIRO, R. V.. Rhizobacteria improve sugarcane growth and photosynthesis under well-watered conditions. Annals of Applied Biology, v. 172, n. 3, p. 309-320, . (08/57495-3, 14/13412-8)

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