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Validation of a cost-effective strategy for tracking rare erythrocyte and platelet phenotypes aiming to organize a rare blood donor program in São Paulo

Grant number: 14/50250-6
Support Opportunities:Research Grants - Research in Public Policies for the National Health Care System (PP-SUS)
Duration: July 01, 2014 - January 31, 2016
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Convênio/Acordo: CNPq - PPSUS
Principal Investigator:Ester Cerdeira Sabino
Grantee:Ester Cerdeira Sabino
Host Institution: Instituto de Medicina Tropical de São Paulo (IMT). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Goals: 1- Validate a high-throughput genotyping strategy using OpenArray® equipment to determine most important erythrocyte and pletelet antigens, in order to make this methodology suitable for tracking rare donors, 2- Elaborate a customized software; to register the donors and to compile all genotyping data. 3- Molecular characterization of sickle cell patients with RhD/RhCE variants, in order to provide compatible blood units. Methods: Automated DNA extraction of 5000 repeat donors. Genotyping using Openarray® equipment for 32 alleles which codify erythrocyte and platelet antigens (MNS, Lutheran, Kell, Duffy, Kidd, Diego, Cartwright, Dombrock, Colton, XK, Cromer, KNOPS, JMH, HPA 1-5 e 15). Complete interfacing of the data to a customized software.Next generation sequencing (IonTorrent) of RhD and RhCE genes of sickle cell patients with serologic suspected variants. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DINARDO, CARLA L.; KELLY, SHANNON; DEZAN, MARCIA R.; RIBEIRO, INGRID H.; CASTILHO, SHIRLEY L.; SCHIMIDT, LUCIANA C.; VALGUEIRO, MARIA DO C.; PREISS, LILIANA R.; CUSTER, BRIAN; SABINO, ESTER C.; et al. Diversity of RH and transfusion support in Brazilian sickle cell disease patients with unexplained Rh antibodies. Transfusion, v. 59, n. 10, . (14/50250-6)
DEZAN, MARCIA R.; RIBEIRO, INGRID HELENA; OLIVEIRA, VALERIA B.; VIEIRA, JULIANA B.; GOMES, FRANCISCO C.; FRANCO, LUCAS A. M.; VARUZZA, LEONARDO; RIBEIRO, ROBERTO; CHINOCA, KAREN ZIZA; LEVI, JOSE EDUARDO; et al. RHD and RHCE genotyping by next-generation sequencing is an effective strategy to identify molecular variants within sickle cell disease patients. BLOOD CELLS MOLECULES AND DISEASES, v. 65, p. 8-15, . (14/50250-6)
OLIVEIRA, V. B.; DEZAN, M. R.; GOMES, F. C. A.; MENOSI GUALANDRO, S. F.; KRIEGER, J. E.; PEREIRA, A. C.; MARSIGLIA, J. D.; LEVI, J. E.; ROCHA, V.; MENDRONE-JUNIOR, A.; et al. -318C/T polymorphism of the CTLA-4 gene is an independent risk factor for RBC alloimmunization among sickle cell disease patients. INTERNATIONAL JOURNAL OF IMMUNOGENETICS, v. 44, n. 5, p. 219-224, . (14/50250-6)

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