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Validation of a cost-effective strategy for tracking rare erythrocyte and platelet phenotypes aiming to organize a rare blood donor program in São Paulo


Goals: 1- Validate a high-throughput genotyping strategy using OpenArray® equipment to determine most important erythrocyte and pletelet antigens, in order to make this methodology suitable for tracking rare donors, 2- Elaborate a customized software; to register the donors and to compile all genotyping data. 3- Molecular characterization of sickle cell patients with RhD/RhCE variants, in order to provide compatible blood units. Methods: Automated DNA extraction of 5000 repeat donors. Genotyping using Openarray® equipment for 32 alleles which codify erythrocyte and platelet antigens (MNS, Lutheran, Kell, Duffy, Kidd, Diego, Cartwright, Dombrock, Colton, XK, Cromer, KNOPS, JMH, HPA 1-5 e 15). Complete interfacing of the data to a customized software.Next generation sequencing (IonTorrent) of RhD and RhCE genes of sickle cell patients with serologic suspected variants. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DINARDO, CARLA L.; KELLY, SHANNON; DEZAN, MARCIA R.; RIBEIRO, INGRID H.; CASTILHO, SHIRLEY L.; SCHIMIDT, LUCIANA C.; VALGUEIRO, MARIA DO C.; PREISS, LILIANA R.; CUSTER, BRIAN; SABINO, ESTER C.; et al. Diversity of RH and transfusion support in Brazilian sickle cell disease patients with unexplained Rh antibodies. Transfusion, v. 59, n. 10, . (14/50250-6)
DEZAN, MARCIA R.; RIBEIRO, INGRID HELENA; OLIVEIRA, VALERIA B.; VIEIRA, JULIANA B.; GOMES, FRANCISCO C.; FRANCO, LUCAS A. M.; VARUZZA, LEONARDO; RIBEIRO, ROBERTO; CHINOCA, KAREN ZIZA; LEVI, JOSE EDUARDO; et al. RHD and RHCE genotyping by next-generation sequencing is an effective strategy to identify molecular variants within sickle cell disease patients. BLOOD CELLS MOLECULES AND DISEASES, v. 65, p. 8-15, . (14/50250-6)
OLIVEIRA, V. B.; DEZAN, M. R.; GOMES, F. C. A.; MENOSI GUALANDRO, S. F.; KRIEGER, J. E.; PEREIRA, A. C.; MARSIGLIA, J. D.; LEVI, J. E.; ROCHA, V.; MENDRONE-JUNIOR, A.; et al. -318C/T polymorphism of the CTLA-4 gene is an independent risk factor for RBC alloimmunization among sickle cell disease patients. INTERNATIONAL JOURNAL OF IMMUNOGENETICS, v. 44, n. 5, p. 219-224, . (14/50250-6)

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