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Molecular aspects of the energetic metabolism in the brain of birds, mice and ruminants - quantification of the genetic expression of key proteins of the glucose metabolism. Species analysis - specific path


Recent results from our lab showed that there are two binding sites (A and B) of hexokinase to brain mitochondria, according to release with glucose-6-P (Cerqueira César and Wilson, 2002). The ratio A:B sites varied in a species - specific way. It was 90% A: 10% B in rat brain and 60% A: 40% B in rabbit and bovine brain, respectively. Later we detected that this species - specific variation, was due to differences in expression of VDAC isoforms (voltage dependent anion channel). VDAC 1 ans VDAC 2 were more expressed in bovine brain in comparison with rat brain (Cerqueira César and Wilson, 2004). Results from our lab that relative to the mRNA for VDAC 3, mRNAs for both VDAC 1 and VDAC 2 were more highly expressed in bovine brain than in rat brain (Cerqueira César and Wilson, 2004). Our results, with those from Shinohara et al. (2000) were the first to do a comparative quantitation between mRNA levels of the different VDAC isoforms in tissues from different species. The theory we are proposing is that there is a relationship between blood glucose levels and the species - specific expression of A and B hexokinase binding sites to brain mitochondria. Another purpose of this study is to determine a possible species - specific differential expression of VDAC, hexokinase and glucose transporters (GLUT). The biological tissues we are going to study are brain tissues from bovine (57 mg/dl blood glucose levels), rat (73 mg/dl) and chicken (168 mg/dl). We are looking for brain molecular adaptations to survive with low (bovine) and high blood glucose (chicken) and suggest molecular therapies for hiperglicemic ou hipoglicemic diseases in humans and monogastrics. Add to that the understanding of VDAC - hexokinase interaction is highly important, because it is a key point of apoptosis control. The proapoptotic protein Bax and hexokinase compete by the same mitochondrial binding site. Proteomic analysis by mass spectrometry will be done at the Thomson laboratory of mass spectrometry (UNICAMP) in cooperation with Prof. Marcos Eberlin. We will be looking for the identification of VDAC isoforms in chicken, bovine and rat brain. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
POLETI, MIRELE DAIANA; TESCH, ANDREA CRISTINA; CREPALDI, CARLA ROSSINI; MARTINS FERREIRA SOUZA, GUSTAVO HENRIQUE; EBERLIN, MARCOS NOGUEIRA; CESAR, MARCELO DE CERQUEIRA. Relationship Between Expression of Voltage-Dependent Anion Channel (VDAC) Isoforms and Type of Hexokinase Binding Sites on Brain Mitochondria. JOURNAL OF MOLECULAR NEUROSCIENCE, v. 41, n. 1, p. 48-54, . (04/03170-5)

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