Application of biocatalysis in organic synthesis using whole cells of microorganisms

Abstract

Aim is to develop methodology for conducting reactions racemic deracemization ofsecondary alcohols in order to obtain alcohols with high enantiomeric purity using whole cells of microorganisms, especially strains of yeasts and fungi. Establish the structural coverage of alcohols that suit the requirements of the active site of enzymes contained in microorganisms, such as withdrawing groups or donors positioned near hydroxyl function, alkyl substituents, aromatics, esters, etc. Beyond the effect of spatial accumulation of substituents around the alcohol function. To study the mechanism of the process and compare with the operation in the literature, exploring variables such as substrate concentration, pH, temperature, aerobic or anaerobic environment, strains and conditioning prior to addition of substrate. Apply deracemization methodology for the synthesis of molecules relevant alcohols from an economic standpoint as pharmacological activity. Perform reactions that allow execution preparative scale.The aim is to screen microorganisms, especially strains of yeast and fungi with the potential to effect reduction of the double bond of allylic alcohols. To establish the structural requirement of the double C = C bonds such as unsaturated substituents in position and influence of stereochemistry (Z) or (E) the biocatalytic process. To determine the occurrence (or not) the oxidation of allyl alcohol over the course of the reaction, a tandem process involving oxidation of the alcohol followed by reduction of the C=C double and intermediate aldehyde, or vice versa. Optimize the conditions of the reaction medium to determine the most efficient process, as the etching time of the strains prior to the addition of substrates , presence or absence of oxygen, temperature, pH, substrate concentration, among other factors. An optimized approach to carry out the time scaling aimed at the completion of the reaction preparative scale. (AU)