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Sugarcane gene functional analysis in transgenic rice and lignin modified sugarcane in order to cellulosic ethanol production

Abstract

Currently, one of the most important priorities for the sugarcane cultivars development used as renewable energy source is the lignocellulosic biomass modification; meaning, change the principal negative factor for the ethanol cellulosic production, the lignin. Continuing the BIOEN project coordinated by Prof. Dr. Paulo Mazzafera (proc. FAPESP 08/58035-6), this proposal aims to evaluate functionally two transcript factor and a dirigent protein sugarcane genes, presumably related to the lignin biosynthesis, via heterologous expression in rice and, at the same time, engender genetic modified sugarcane to obtain plants with the lignin content modified. One known the existence of the potential to improve the saccharification proceeding in plants increasing the proportion of sinapyl (S) subunit incorporate to lignin comparing to guaicyl (G) subunit and maybe the hidroxiphenyl (H) subunit, but it is underexplored and yet it have not probed in sugarcane. Therefore, the genes hidroxicinamoyl CoA:shiquimate/quinate hidroxicinamoyl transferase (HCT) and 5-hidroxylase ferulic acid (F5H) will be overexpressed in sugarcane to expect the increasing of S/G and or S/H ration in the plant. From the achievements proposed here, we expect establishing background for hereafter to be developed in the Sugarcane Center, IAC-Ribeirão Preto, highly qualified in sugarcane breeding program, the sugarcane cultivars production with bioenergy potential for genetic improvement via genetic transformation. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BOER MARTINS, ALEXANDRE PALMA; BRITO, MICHAEL DOS SANTOS; MAYER, JULIANA LISCHKA S.; PORTILLA LLERENA, JUAN PABLO; OLIVEIRA, JASMIM FELIPE; TAKAHASHI, NATALIA CONGALVES; CARLIN, SAMIRA DOMINGUES; ALINE FLORES BORGES, DENISELE NEUZA; ANDRADE, LARISSA MARA; PEIXOTO-JUNIOR, RAFAEL FAVERO; DE SOUZA GOLDMAN, MARIA HELENA; MAZZAFERA, PAULO; CRESTE, SILVANA; NOBILE, PAULA MACEDO. Ectopic expression of sugarcane SHINE changes cell wall and improves biomass in rice. BIOMASS & BIOENERGY, v. 119, p. 322-334, DEC 2018. Web of Science Citations: 1.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
Filed patent(s) as a result of this research project

Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação BR1020180704168 - Instituto Agronômico de Campinas (IAC) . Solicitação em análise e dentro do prazo legal de sigilo previsto na legislação - October 2018, 03