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The effect of poly(vinylidene-trifluoroethylene)/barium titanate composite in the repair of bone defects in calvaria of ovariectomized rats


The bone tissue presents regenerative potential, but some diseases can lead to bone loss, such as osteoporosis, that compromised bone strength and quality, predisposing to increased risk of fractures. So, faced with this and other diseases, it becomes important to search for new methodologies that aim bone deposition. In vitro researchs comparing the poly(vinylidene fluoride-trifluoroethylene)/barium titanate (P(VDF-TrFE)/BT) membrane and polytetrafluoroethylene (PTFE) membrane showed favorable results for (P(VDF-TrFE)/BT) for different cell lines: osteoblasts, fibroblasts and keratinocytes. Thus, this study aims to evaluate in vivo the response of bone tissue to (P(VDF-TrFE)/BT) membrane and compare it to the PTFE membrane (control) in the calvaria of Wistar rats with experimental model for osteoporosis. Female Wistar rats (300g) will be bilaterally ovariectomized. After 150 days will be performed bone defects (5 mm) in the skull and the animals will be divided into three groups with respect to the use or not the membrane in the bone defects: (Group 1) (P(VDF-TrFE)/BT), (Group 2) PTFE membrane, ( Group 3) any kind of membrane. At the end of 4 weeks, the animals will be sacrificed and will be carried out as follows: (1) analysis tomography (micro-CT) of the defects previously created, (2) histological analysis in non-decalcified histological sections, (3) the mRNA expression of runt-related transcription factor 2 (RUNX2), alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OC), osteopontin (OPN), osterix (OSX), osteoprotegerin (OPG), receptor activator of nuclear factor kappa B ligand (RANKL), receptor activator of nuclear factor-kappa B (RANK), Cathepsin K and matrix metalloproteinase-9 (MMP-9) by real-time PCR. These bone markers will be also detected in tissue samples taken from the skull bone at surgery for placement of the membrane in order to compare the bone formation on the membranes with pre-existing bone tissue. The number of animals for each group will be 5 (n=5). Statistical tests to analyze the numerical results will be selected according to the distribution of samples. (AU)

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(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SCALIZE, PRISCILLA HAKIME; BOMBONATO-PRADO, KARINA F.; DE SOUSA, LUIZ GUSTAVO; ROSA, ADALBERTO LUIZ; BELOTI, MARCIO MATEUS; SEMPRINI, MARISA; GIMENES, ROSSANO; DE ALMEIDA, ADRIANA L. G.; DE OLIVEIRA, FABIOLA SINGARETTI; HALLAK REGALO, SIMONE CECILIO; et al. Poly(Vinylidene Fluoride-Trifluorethylene)/barium titanate membrane promotes de novo bone formation and may modulate gene expression in osteoporotic rat model. JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, v. 27, n. 12, . (14/02984-0)

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