Advanced search
Start date

Development of novel methodology for the routine monitoring of monohydroxy metabolites of polycyclic aromatic hydrocarbons in numerous urine samples

Grant number: 14/00986-6
Support Opportunities:Research Grants - Visiting Researcher Grant - International
Duration: May 08, 2014 - August 07, 2014
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
Principal Investigator:Fernando Barbosa Júnior
Grantee:Fernando Barbosa Júnior
Visiting researcher: Andres Dobal Campiglia
Visiting researcher institution: University of Central Florida (UCF), United States
Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Reducing the sample temperature offers several advantages to molecular luminescence (fluorescence and/or phosphorescence) spectroscopy. Analysis at cryogenic temperatures often improves spectral resolution, increases emission quantum yields and minimizes - or even eliminates - complications with oxygen quenching and energy transfer. Although spectral resolution improvement is almost always observed with lowering the temperature to 77 K or below, temperature effects on spectral narrowing are particularly noticeable in Shpol'skii spectroscopy. This technique combines the inherent sensitivity of fluorescence and phosphorescence with the selectivity of vibrationally resolved spectra. Unfortunately, the Shpol'skii phenomenon requires a specific guest-host combination that has confined Shpol'skii spectroscopy to the analysis of polycyclic aromatic hydrocarbons (PAHs) that are compatible with non-polar matrixes (n-alkanes). PAHs are important environmental pollutants of great environmental and toxicological importance.The end goal of this proposition is to generalize the methods of Shpol'skii spectroscopy to a variety of polar compounds by using polar solvents. Initial work is proposed here to investigate the Shpol'skii behavior of monohydroxy metabolites of PAHs (OH-PAHs) in frozen organic alcohols, provide a more general model for Shpol'skii spectroscopy and allow more widespread use of the methodology. Due to the short average elimination lifetime from the body, the quantitative determination of OH-PAHs in urine samples provides accurate information on recent environmental exposure to PAHs. Although chromatographic techniques provide adequate limits of detection for OH-PAHs in urine samples, their experimental procedures are time consuming and expensive. The research presented here will provide easy-to-use and cost-effective methodology well-suited methodology for data collection from statistically meaningful population sizes. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
Articles published in other media outlets (0 total):
More itemsLess items

Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MATHEUS GALLIMBERTI; BRUNO A. ROCHA; VANESSA C. O. SOUZA; ANDRES D. CAMPIGLIA; FERNANDO BARBOSA JR. Determination of Bisphenol A in Paper Products by Synchronous Fluorescence Spectoscopy and Estimation of Daily Exposure. Journal of the Brazilian Chemical Society, v. 31, n. 10, p. 2146-2154, . (18/24069-3, 14/00986-6, 12/03465-1, 13/23710-3)
ALFARHANI, BASSAM; AL-TAMEEMI, MAHA; SCHENONE, AGUSTINA V.; GOICOECHEA, HECTOR C.; BARBOSA, JR., FERNANDO; CAMPIGLIA, ANDRES D.. Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes. Microchemical Journal, v. 129, p. 83-89, . (14/00986-6)
MOORE, ANTHONY F. T.; GOICOECHEA, HECTOR C.; BARBOSA, JR., FERNANDO; CAMPIGLIA, ANDRES D.. Parallel Factor Analysis of 4.2 K Excitation-Emission Matrices for the Direct Determination of Dibenzopyrene Isomers in Coal-Tar Samples with a Cryogenic Fiber-Optic Probe Coupled to a Commercial Spectrofluorimeter. Analytical Chemistry, v. 87, n. 10, p. 5232-5239, . (14/00986-6, 12/03465-1)
BORGES, ENDLER MARCEL; VOLMER, DIETRICH A.; GALLIMBERTI, MATHEUS; DE SOUZA, DEISE FERREIRA; DE SOUZA, EDSON LUIZ; BARBOSA, JR., FERNANDO. Evaluation of macro- and microelement levels for verifying the authenticity of organic eggs by using chemometric techniques. ANALYTICAL METHODS, v. 7, n. 6, p. 2577-2584, . (11/08467-0, 14/00986-6, 11/07416-2, 11/07466-0, 12/03465-1)

Please report errors in scientific publications list by writing to: