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Enzyme ligand: new models of screening

Grant number: 13/01710-1
Support Opportunities:Research Projects - Thematic Grants
Duration: February 01, 2014 - January 31, 2018
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal Investigator:Quezia Bezerra Cass
Grantee:Quezia Bezerra Cass
Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
Pesquisadores principais:
Carmen Lúcia Cardoso
Associated researchers:Arthur Henrique Cavalcante de Oliveira ; Dulce Helena Ferreira de Souza ; Emerson Rodrigues de Camargo
Associated scholarship(s):16/23794-0 - Stereochemistry of complex natural compounds by vibrational optical activity: acetogenins, BP.PD
16/14482-5 - Nanofractionation LC-MS/MS and at-line Bioassays for identification of bioactive components in Natural products extracts, BE.PQ
16/02873-0 - Acetylcholinesterase and beta-secretase 1: a study of co-immobilization conditions for screening of ligands, BP.DR
+ associated scholarships 15/25755-0 - Immobilization and characterization of the enzyme trypsin reactor for use in proteomic analysis, BP.IC
14/21852-8 - Acetylcholinesterase from Atta Sexdens (leaf-cutter ants): development of a system to expression, purification and functional characterization, BP.PD
14/11640-3 - LC-MS / 2D methods for screening ligands for evaluating enzyme inhibitory activity and identification of active compounds in crude extracts, BP.PD
14/06907-0 - Immobilization nucleoside diphosphate kinase (NDK) enzyme Leishmania major: the study of conditions for the application in the screening of ligands, BP.DD
13/02054-0 - Enzyme immobilization: new tools for ligand screening, BP.PD - associated scholarships

Abstract

Several high-throughput assay strategies have been developed for the discovery of protein ligand; among them, frontal (FAC), Zonal (LZC) and nonlinear (NLC) chromatography with immobilized enzymes. The outstanding advantage of bioaffinity chromatography is the easy thermodynamics, kinetics and chemical ligand characterization, in an on-line hyphenated system. This project addresses the development and characterization of enzyme-based stationary phases, and the application of these phases in the development of new screening technologies. The use of ligand fishing and missing peak chromatography and or ligand fishing and dereplication by MS/MS approaches will be explored. A series of enzymes, including the cholinesterases, nucleoside-diphosphate kinase, phosphenolpyruvate carboxykinase, angiotensinconverting enzyme and Xanthine oxidase, were selected as the target for the herein described project. (AU)

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Scientific publications (43)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ADRIANA F. L. VILELA; BÁRBARA M. FRUGERI; ANDRÉ L. F. SARRIA; RODRIGO O. S. KITAMURA; JOÃO B. FERNANDES; MARIA F. G. F. SILVA; QUEZIA B. CASS; CARMEN L. CARDOSO. Aminonaphthoquinone Mannich Bases Derived from Lawsone and Their Copper(II) Complex Derivatives: Synthesis and Potential Cholinesterase Inhibitors as Identified by On-flow Assay. Journal of the Brazilian Chemical Society, v. 27, n. 3, p. 534-545, . (06/58043-3, 13/01710-1, 07/06393-3, 07/07196-7)
CALIL, FELIPE ANTUNES; LIMA, JULIANA MARIA; CAVALCANTE DE OLIVEIRA, ARTHUR HENRIQUE; MARIOTINI-MOURA, CHRISTIANE; RANGEL FIETTO, JULIANA LOPES; CARDOSO, CARMEN LUCIA. Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay. JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, . (13/01710-1, 08/04371-5)
MAGALHAES, LUANA; CAVALCANTE DE OLIVEIRA, ARTHUR HENRIQUE; VASCONCELLOS, RAPHAEL DE SOUZA; MARIOTINI-MOURA, CHRISTIANE; FIRMINO, RAFAELA DE CASSIA; RANGEL FIETTO, JULIANA LOPES; CARDOSO, CARMEN LUCIA. Label-free assay based on immobilized capillary enzyme reactor of Leishmania infantum nucleoside triphosphate diphosphohydrolase (LicNTPDase-2-ICER-LC/UV). JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1008, p. 98-107, . (13/01710-1, 12/10749-6)
MANTOANI, SUSIMAIRE P.; CHIERRITO, TALITA P. C.; VILELA, ADRIANA F. L.; CARDOSO, CARMEN L.; MARTINEZ, ANA; CARVALHO, IVONE. Novel Triazole-Quinoline Derivatives as Selective Dual Binding Site Acetylcholinesterase Inhibitors. Molecules, v. 21, n. 2, . (13/50788-3, 13/01710-1, 12/14114-5, 12/04054-5)
LOPES, ADRIANA FERREIRA; CARDOSO, CARMEN LUCIA. An on-flow assay for screening of beta-secretase ligands by immobilised capillary reactor-mass spectrometry. ANALYTICAL METHODS, v. 9, n. 14, p. 2189-2196, . (13/01710-1)
LIMA, JULIANA M.; SEIDL, CLAUDIA; CUNHA, ELISE M. F.; OLIVEIRA, ARTHUR H. C.; CARDOSO, CARMEN L.. On-Flow Ligand Screening Assay Based on Immobilized Nucleoside Diphosphate Kinase B from Homo sapiens. Journal of the Brazilian Chemical Society, v. 30, n. 11, SI, p. 2308-2317, . (14/50249-8, 13/01710-1, 14/06907-0, 14/11640-3)
AQUINO, AMANDA J.; ALVES, THAYANA DA C.; OLIVEIRA, V, REGINA; FERREIRA, ANTONIO G.; CASS, QUEZIA B.. Chemical secondary metabolite profiling of Bauhinia longifolia ethanolic leaves extracts. INDUSTRIAL CROPS AND PRODUCTS, v. 132, p. 59-68, . (13/01710-1)
IZADORA L. FURLANI; BRUNO S. AMARAL; REGINA V. OLIVEIRA; QUEZIA B. CASS. Imobilização enzimática: conceito e efeitos na proteólise. Química Nova, v. 43, n. 4, p. 463-473, . (18/03035-3, 14/50249-8, 15/25755-0, 13/01710-1)
GONCALVES NETO, GERALDO S.; DA COSTA, ROMARIO P.; ZANINI, CAMILA L.; AGUIAR, ANNA CAROLINE C.; DE SOUZA, JULIANA O.; DE SOUZA, GUILHERME E.; SEVERINO, RICHELE P.; CASS, QUEZIA B.; CRUZ, FABIO C.; OLIVA, GLAUCIUS; et al. Chemical Prospection of Qualea grandiflora Mart. Fruit and Stem Extracts and Their in vitro and in vivo Antiplasmodial Activity. Journal of the Brazilian Chemical Society, v. 31, n. 7, p. 1475-1484, . (13/01710-1, 13/07600-3)
SEIDL, CLAUDIA; LOPES VILELA, ADRIANA FERREIRA; LIMA, JULIANA MARIA; LEME, GABRIEL MAZZI; CARDOSO, CARMEN LUCIA. A novel on-flow mass spectrometry-based dual enzyme assay. Analytica Chimica Acta, v. 1072, p. 81-86, . (13/01710-1, 14/50249-8)
LOPES VILELA, ADRIANA FERREIRA; CARDOSO, CARMEN LUCIA; MATEO, CESAR. An immobilized acetylcholinesterase as test system to screen new inhibitor drugs to treat Alzheimer's disease. SENSORS AND ACTUATORS B-CHEMICAL, v. 278, p. 196-201, . (16/15037-5, 13/01710-1)
VANZOLINI, KENIA L.; VIEIRA, LUCAS C. C.; CORREA, ARLENE. G.; MOADDEL, RUIN; CASS, QUEZIA B.. Acetylcholinesterase immobilized on modified magnetic beads as a tool for screening a compound library. Microchimica Acta, v. 182, n. 13-14, p. 2209-2213, . (13/02054-0, 13/01710-1)
DE MORAES, MARCELA CRISTINA; CARDOSO, CARMEN LUCIA; CASS, QUEZIA BEZERRA. Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions. RONTIERS IN CHEMISTR, v. 7, p. 16-pg., . (13/01710-1, 14/50249-8)
VILELA, ADRIANA F. L.; FRUGERI, BARBARA M.; SARRIA, ANDRE L. F.; KITAMURA, RODRIGO O. S.; FERNANDES, JOAO B.; SILVA, MARIA F. G. F.; CASS, QUEZIA B.; CARDOSO, CARMEN L.. Aminonaphthoquinone Mannich Bases Derived from Lawsone and Their Copper( II) Complex Derivatives: Synthesis and Potential Cholinesterase Inhibitors as Identified by On-flow Assay. Journal of the Brazilian Chemical Society, v. 27, n. 3, p. 12-pg., . (13/01710-1, 06/58043-3, 07/07196-7, 07/06393-3)
DE LIMA, BRUNA R.; LIMA, JULIANA M.; MACIEL, JESSICA B.; VALENTIM, CAROLINA Q.; NUNOMURA, RITA DE CSSIA S.; LIMA, EMERSON S.; KOOLEN, HECTOR H. F.; DE SOUZA, AFONSO DUARTE L.; PINHEIRO, MARIA LUCIA B.; CASS, QUEZIA B.; et al. Synthesis and Inhibition Evaluation of New Benzyltetrahydroprotoberberine Alkaloids Designed as Acetylcholinesterase Inhibitors. RONTIERS IN CHEMISTR, v. 7, p. 12-pg., . (13/01710-1, 14/50249-8)
DE LIMA, BRUNA R.; LIMA, JULIANA M.; MACIEL, JESSICA B.; VALENTIM, CAROLINA Q.; NUNOMURA, RITA DE CSSIA S.; LIMA, EMERSON S.; KOOLEN, HECTOR H. F.; DE SOUZA, AFONSO DUARTE L.; PINHEIRO, MARIA LUCIA B.; CASS, QUEZIA B.; et al. Synthesis and Inhibition Evaluation of New Benzyltetrahydroprotoberberine Alkaloids Designed as Acetylcholinesterase Inhibitors. FRONTIERS IN CHEMISTRY, v. 7, . (14/50249-8, 13/01710-1)
DE JESUS PRADO, VILMA MENEZES; DE JESUS, RAPHAEL AMANCIO; ANDRADE OLIVEIRA, JULIO MANOEL; BLANK, ARIE FITZGERALD; BEZERRA, DANIEL PEREIRA; PEREIRA SOARES, MILENA BOTELHO; SILVA, VALDENIZIA RODRIGUES; SANTOS, LUCIANO DE SOUZA; CARDOSO, CARMEN LUCIA; LOPES VILELA, ADRIANA FERREIRA; et al. Biological studies and chromatograms aided by chemometric analysis in evaluation of seasonality and extraction method of Croton grewioides extracts. Rev. bras. Bot., v. 45, n. 2, p. 12-pg., . (14/50249-8, 14/11640-3, 13/01710-1)
SCATENA, GABRIEL S.; CASSIANO, NEILA M.; NETTO, CHAQUIP D.; COSTA, PAULO R. R.; CASS, QUEZIA B.; BATISTA, JOAO M., JR.. Preparative chiral separation and absolute configuration of the synthetic pterocarpanquinone LQB-118. CHIRALITY, v. 29, n. 5, p. 5-pg., . (13/01710-1, 14/25222-9, 15/07089-2)
RODRIGUES DE MELO, RICARDO; CARLOS ALNOCH, ROBSON; LOPES VILELA, ADRIANA FERREIRA; DE SOUZA, EMANUEL MALTEMPI; KRIEGER, NADIA; RULLER, ROBERTO; SATO, HELIA HARUMI; MATEO, CESAR. New Heterofunctional Supports Based on Glutaraldehyde-Activation: A Tool for Enzyme Immobilization at Neutral pH. Molecules, v. 22, n. 7, . (13/01710-1, 16/15037-5)
RODRIGUES, M. V. N.; CORREA, R. S.; VANZOLINI, K. L.; SANTOS, D. S.; BATISTA, A. A.; CASS, Q. B.. Characterization and screening of tight binding inhibitors of xanthine oxidase: an on-flow assay. RSC ADVANCES, v. 5, n. 47, p. 37533-37538, . (13/01710-1, 09/08131-1, 13/26559-4)
RAPHAEL A. DE JESUS; VILMA M. J. PRADO; VANDERSON S. PINTO; VALDENIZIA R. SILVA; LUCIANO S. SANTOS; PAULO C. L. NOGUEIRA; SANDRO NAVICKIENE; EDENIR R. PEREIRA-FILHO; ARIE F. BLANK; DANIEL P. BEZERRA; et al. Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill) N. E. Brown and Biological Activities. Journal of the Brazilian Chemical Society, v. 30, n. 5, p. 978-987, . (13/01710-1, 14/11640-3)
VANZOLINI, K. L.; SPRENGER, R. DA F.; LEME, G. M.; MORAES, V. R. DE S.; VILELA, A. F. L.; CARDOSO, C. L.; CASS, Q. B.. Acetylcholinesterase affinity-based screening assay on Lippia gracilis Schauer extracts. Journal of Pharmaceutical and Biomedical Analysis, v. 153, p. 232-237, . (13/02054-0, 13/01710-1, 16/24957-0, 14/50249-8)
DO AMARAL, BRUNO S.; CORREA, KATIA CELINA S.; CORREA, ARLENE G.; DE SOUZA, DULCE H. F.; CASS, QUEZIA B.. Direct Assay to Evaluate Phosphoenolpyruvate Carboxykinase Activity. Journal of the Brazilian Chemical Society, v. 30, n. 10, SI, p. 2105-2113, . (14/50249-8, 13/01710-1)
CANZI, EDIONE F.; LOPES, BIANCA REBELO; ROBELDO, THAIANE; BORRA, RICARDO; DA SILVA, MARIA FATIMA G. F.; OLIVEIRA, V, REGINA; MAIA, BEATRIZ HELENA N. S.; CASS, QUEZIA B.. Prostaglandins E-2 and F-2 alpha levels in human menstrual fluid by online Solid Phase Extraction coupled to Liquid Chromatography tandem Mass Spectrometry (SPE-LC-MS/MS). JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1109, p. 60-66, . (13/01710-1)
DE JESUS, RAPHAEL A.; PRADO, VILMA M. J.; PINTO, VANDERSON S.; SILVA, VALDENIZIA R.; SANTOS, LUCIANO S.; NOGUEIRA, PAULO C. L.; NAVICKIENE, SANDRO; PEREIRA-FILHO, EDENIR R.; BLANK, ARIE E.; BEZERRA, DANIEL P.; et al. Application of LC-DAD Metabolic Fingerprinting in Combination with PCA for Evaluation of Seasonality and Extraction Method on the Chemical Composition of Accessions from Lippia alba (Mill.) N. E. Brown and Biological Activities. Journal of the Brazilian Chemical Society, v. 30, n. 5, p. 978-987, . (13/01710-1, 14/11640-3)
DE MORAES, MARCELA CRISTINA; CARDOSO, CARMEN LUCIA; CASS, QUEZIA BEZERRA. Solid-Supported Proteins in the Liquid Chromatography Domain to Probe Ligand-Target Interactions. FRONTIERS IN CHEMISTRY, v. 7, . (14/50249-8, 13/01710-1)
VILMA M. J. PRADO; RAPHAEL A. DE JESUS; JULIO M. A. OLIVEIRA; CAMILA S. A. PEREIRA; ARIE F. BLANK; EDENIR R. PEREIRA-FILHO; QUEZIA B. CASS; JULIANA M. DE LIMA; ANTONIO G. FERREIRA; PAULO C. L. NOGUEIRA; et al. Evaluation of the Seasonality and Extraction Method on the Polar Extracts of Croton grewioides Baill. by Chromatogram Fingerprinting and Isolation of a New Triglycosylated Flavonoid. Journal of the Brazilian Chemical Society, v. 32, n. 2, p. 385-394, . (14/11640-3, 13/01710-1)
VANZOLINI, KENIA LOURENCO; AINSWORTH, STUART; BRUYNEEL, BEN; HERZIG, VOLKER; SERAUS, MITCHELL G. L.; SOMSEN, GOVERT W.; CASEWELL, NICHOLAS R.; CASS, QUEZIA BEZERRA; KOOL, JEROEN. Rapid ligand fishing for identification of acetylcholinesterase-binding peptides in snake venom reveals new properties of dendrotoxins. Toxicon, v. 152, p. 1-8, . (15/18504-0, 13/01710-1)
DOS SANTOS, JR., FERNANDO M.; BICALHO, KEYLLA U.; CALISTO, ITALO H.; SCATENA, GABRIEL S.; FERNANDES, JOAO B.; CASS, QUEZIA B.; BATISTA, JR., JOAO M.. Scope of the 2(5H)-furanone helicity rule: a combined ECD, VCD, and DFT investigation. ORGANIC & BIOMOLECULAR CHEMISTRY, v. 16, n. 24, p. 4509-4516, . (15/07089-2, 13/01710-1, 14/25222-9, 10/18832-4, 12/25299-6, 16/23794-0)
DO AMARAL, BRUNO S.; DA SILVA, LARISSA R. G.; VALVERDE, ALESSANDRA L.; DE SOUSA, LORENA R. F.; SEVERINO, RICHELE P.; DE SOUZA, DULCE H. F.; CASS, QUEZIA B.. Phosphoenolpyruvate carboxykinase from T. cruzi magnetic beads affinity-based screening assays on crude plant extracts from Brazilian Cerrado. Journal of Pharmaceutical and Biomedical Analysis, v. 193, . (13/01710-1, 14/50249-8)
CORNELIO, VIVIAN ESTEVAM; DE MORAES, MARCELA CRISTINA; DOMINGUES, VANESSA DE CASSIA; FERNANDES, JOAO BATISTA; DAS GRACAS FERNANDES DA SILVA, MARIA FATIMA; CASS, QUEZIA BEZERRA; VIEIRA, PAULO CEZAR. Cathepsin D immobilized capillary reactors for on-flow screening assays. Journal of Pharmaceutical and Biomedical Analysis, v. 151, p. 252-259, . (13/01710-1, 09/17538-8)
SCATENA, GABRIEL S.; CASSIANO, NEILA M.; NETTO, CHAQUIP D.; COSTA, PAULO R. R.; CASS, QUEZIA B.; BATISTA, JR., JOAO M.. Preparative chiral separation and absolute configuration of the synthetic pterocarpanquinone LQB-118. Chirality, v. 29, n. 5, p. 167-171, . (13/01710-1, 14/25222-9, 15/07089-2)
MEDINA, REBECA P.; ARAUJO, ANGELA R.; BATISTA JR, JOAO M.; CARDOSO, CARMEN L.; SEIDL, CLAUDIA; VILELA, ADRIANA F. L.; DOMINGOS, V, HELORI; COSTA-LOTUFO, V, LETICIA; ANDERSEN, RAYMOND J.; SILVA, DULCE H. S.. Botryane terpenoids produced by Nemania bipapillata, an endophytic fungus isolated from red alga Asparagopsis taxiformis - Falkenbergia stage. SCIENTIFIC REPORTS, v. 9, . (13/01710-1, 14/50926-0, 15/17177-6, 13/07600-3)
LOPES, BIANCA REBELO; BARREIRO, JULIANA CRISTINA; CASS, QUEZIA BEZERRA. Bioanalytical challenge: A review of environmental and pharmaceuticals contaminants in human milk. Journal of Pharmaceutical and Biomedical Analysis, v. 130, p. 8-pg., . (13/01710-1)
CALIL, FELIPE ANTUNES; LIMA, JULIANA MARIA; CAVALCANTE DE OLIVEIRA, ARTHUR HENRIQUE; MARIOTINI-MOURA, CHRISTIANE; RANGEL FIETTO, JULIANA LOPES; CARDOSO, CARMEN LUCIA. Immobilization of NTPDase-1 from Trypanosoma cruzi and Development of an Online Label-Free Assay. JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY, v. 2016, p. 9-pg., . (13/01710-1, 08/04371-5)
RODRIGUES, MARILI V. N.; BARBOSA, ALEXANDRE F.; DA SILVA, JULIA F.; DOS SANTOS, DEBORAH A.; VANZOLINI, KENIA L.; DE MORAES, MARCELA C.; CORREA, ARLENE G.; CASS, QUEZIA B.. 9-Benzoyl 9-deazaguanines as potent xanthine oxidase inhibitors. Bioorganic & Medicinal Chemistry, v. 24, n. 2, p. 226-231, . (13/01710-1, 13/50680-8, 13/07600-3)
DE ALMEIDA, FERNANDO G.; VANZOLINI, KENIA L.; CASS, QUEZIA B.. Angiotensin converting enzyme immobilized on magnetic beads as a tool for ligand fishing. Journal of Pharmaceutical and Biomedical Analysis, v. 132, p. 159-164, . (13/01710-1, 11/00398-9)
BATISTA, ANDREA N. L.; DOS SANTOS, JR., FERNANDO M.; BATISTA, JR., JOAO M.; CASS, QUEZIA B.. Enantiomeric Mixtures in Natural Product Chemistry: Separation and Absolute Configuration Assignment. Molecules, v. 23, n. 2, . (13/01710-1, 16/23794-0, 14/25222-9, 15/07089-2)
ALVIM-, JR., JOEL; LOPES, BIANCA REBELO; CASS, QUEZIA BEZERRA. Simultaneous enantioselective quantification of fluoxetine and norfluoxetine in human milk by direct sample injection using 2-dimensional liquid chromatography-tandem mass spectrometry. Journal of Chromatography A, v. 1451, p. 120-126, . (13/01710-1)
SPRENGER, R. F.; THOMASI, S. S.; FERREIRA, A. G.; CASS, Q. B.; BATISTA JUNIOR, J. M.. Solution-state conformations of natural products from chiroptical spectroscopy: the case of isocorilagin. ORGANIC & BIOMOLECULAR CHEMISTRY, v. 14, n. 13, p. 3369-3375, . (11/11021-3, 13/01710-1, 14/25222-9, 15/07089-2)
DE MORAES, MARCELA CRISTINA; CARDOSO, CARMEN LUCIA; SEIDL, CLAUDIA; MOADDEL, RUIN; CASS, QUEZIA BEZERRA. Targeting Anti-Cancer Active Compounds: Affinity-Based Chromatographic Assays. CURRENT PHARMACEUTICAL DESIGN, v. 22, n. 39, p. 5976-5987, . (13/01710-1)
LIMA, JULIANA MARIA; VIEIRA, PLINIO SALMAZO; CAVALCANTE DE OLIVEIRA, ARTHUR HENRIQUE; CARDOSO, CARMEN LUCIA. Label-free offline versus online activity methods for nucleoside diphosphate kinase b using high performance liquid chromatography. ANALYST, v. 141, n. 15, p. 4733-4741, . (14/06907-0, 13/01710-1, 11/20569-2)
LOPES, BIANCA REBELO; BARREIRO, JULIANA CRISTINA; CASS, QUEZIA BEZERRA. Bioanalytical challenge: A review of environmental and pharmaceuticals contaminants in human milk. Journal of Pharmaceutical and Biomedical Analysis, v. 130, n. SI, p. 318-325, . (13/01710-1)

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