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Proteomic and transcriptomic analysis of Histoplasma capsulatum biofilm and correlation with virulence factors in search of new therapeutic targets

Grant number: 13/05853-1
Support type:Regular Research Grants
Duration: October 01, 2013 - November 30, 2015
Field of knowledge:Health Sciences - Pharmacy
Principal researcher:Ana Marisa Fusco Almeida
Grantee:Ana Marisa Fusco Almeida
Home Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Assoc. researchers:Christiane Pienna Soares ; Dulce Helena Siqueira Silva ; Maria José Soares Mendes Giannini ; Maria Lucia Taylor da Cunha e Mello

Abstract

Histoplasma capsulatum var. capsulatum is a dimorphic fungus that causes histoplasmosis, a respiratory and systemic mycosis. The development of disease depends on the ability of Histoplasma yeast to survive and replicate within alveolar macrophages. In this context, it is known that adherence to host cells is the first step in the colonization and is essential for the establishment of infection. In view of this, some microorganisms adhere to biological and non-biological surfaces to form biofilms. Based on the potential importance of biofilms for survival in the human host and in nature, this study is designed to investigate the biofilm formation by H. capsulatum correlated with their ability to adhere to and/or invade epithelial cells and macrophages. Additionally, there will be an analysis of the proteome and transcriptome reveal that the protein and transcriptional profile differential fungus shaped biofilm and planktonic conditions under. Also set particularities of interaction between host cell and H. capsulatum shaped biofilm and select natural substances of plant origin capable of interfering in the formation of biofilm and the infection of the fungus to host cells. The antifungal substances can interfere with the structure or the destruction of the biofilm and the infection pathogen-host will be selected from collections of natural antifungal molecules obtained in collaboration as Project FAPESP Biota and CNPq Sisbiota with the Institute of Chemistry, UNESP Araraquara. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MEDINA-ALARCON, KAILA PETRONILA; TOBIAS DA SILVA, IARA PENGO; FERIN, GIOVANA GARCIA; PEREIRA-DA-SILVA, MARCELO A.; MARCOS, CAROLINE MARIA; DOS SANTOS, MARIANA BASTOS; REGASINI, LUIS OCTAVIO; CHORILLI, MARLUS; MENDES-GIANNINI, MARIA JOSE S.; PAVAN, FERNANDO ROGERIO; FUSCO-ALMEIDA, ANA MARISA. Mycobacterium tuberculosis and Paracoccidioides brasiliensis Formation and Treatment of Mixed Biofilm In Vitro. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY, v. 11, NOV 1 2021. Web of Science Citations: 0.
PITANGUI, NAYLA DE SOUZA; DE LACORTE SINGULANI, JUNYA; SARDI, JANAINA DE CASSIA ORLANDI; DE SOUZA, PAULA CAROLINA; RODRIGUEZ-ARELLANES, GABRIELA; GARCIA-PEREZ, BLANCA ESTELA; ENGUITA, FRANCISCO JAVIER; PAVAN, FERNANDO R.; TAYLOR, MARIA LUCIA; MENDES-GIANNINI, MARIA JOSE SOARES; FUSCO-ALMEIDA, ANA MARISA. Differential miRNA Expression in Human Macrophage-Like Cells Infected with Histoplasma capsulatum Yeasts Cultured in Planktonic and Biofilm Forms. JOURNAL OF FUNGI, v. 7, n. 1 JAN 2021. Web of Science Citations: 0.
PITANGUI, NAYLA DE SOUZA; SARDI, JANAINA DE CASSIA ORLANDI; VOLTAN, ALINE R.; DOS SANTOS, CLAUDIA T.; DA SILVA, JULHIANY DE FATIMA; DA SILVA, ROSANGELA A. M.; SOUZA, FELIPE O.; SOARES, CHRISTIANE P.; RODRIGUEZ-ARELLANES, GABRIELA; LUCIA TAYLOR, MARIA; MENDES-GIANNINI, MARIA J. S.; FUSCO-ALMEIDA, ANA M. An Intracellular Arrangement of Histoplasma capsulaturn Yeast-Aggregates Generates Nuclear Damage to the Cultured Murine Alveolar Macrophages. FRONTIERS IN MICROBIOLOGY, v. 6, JAN 11 2016. Web of Science Citations: 0.

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