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Saccharomyces cerevisiae as a model for mitochondrial translation studies

Grant number: 13/09482-8
Support Opportunities:Regular Research Grants
Duration: October 01, 2013 - September 30, 2015
Field of knowledge:Biological Sciences - Biochemistry - Metabolism and Bioenergetics
Principal Investigator:Mario Henrique de Barros
Grantee:Mario Henrique de Barros
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Our aim is to investigate mitochondria biogenesis through the study of genes with unknown function. In the last ten years we have characterized the following genes: COX23, COX24, COQ9, COQ10, ATP25, GTF1 and MTG3. They are involved in different aspects of mitochondria biogenesis such as the assembly of cytochrome c oxidase, CoQ function, ATP synthase formation, and mitochondria translation. On the other hand, the similarity between yeast and human respiratory metabolism allows the assessment of human pathological mutations in yeast. The proposal here is to investigate S. cerevisiae genes involved in the mitochondrial translation process, as seem to be the case of YPR116w, YDR065c , YDR115w and others. The project also aim to search for new translational factors through the allotopic expression of the mitochondrial genes COX2 and ATP8. Finally, using yeast as a model, the project aim to search for a human gene ortologous for the yeast amidotransferase connector subunit, which is also involved in the organelle translation process. (AU)

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Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
MODA, BRUNO S.; FERREIRA-JUNIOR, JOSE RIBAMAR; BARROS, MARIO H.. Partial suppression of the respiratory defect of qrs1/her2 glutamyl-tRNA amidotransferase mutants by overexpression of the mitochondrial pentatricopeptide Msc6p. CURRENT GENETICS, v. 62, n. 3, p. 607-617, . (13/09482-8)
BARROS, MARIO H.; TZAGOLOFF, ALEXANDER. Aep3p-dependent translation of yeast mitochondrial ATP8. MOLECULAR BIOLOGY OF THE CELL, v. 28, n. 11, p. 1426-1434, . (13/09482-8, 13/07937-8)
BRADLEY, MICHELLE C.; YANG, KRISTA; FERNANDEZ-DEL-RIO, LUCIA; NGO, JENNIFER; AYER, ANITA; TSUI, HUI S.; NOVALES, NOELLE ALEXA; STOCKER, ROLAND; SHIRIHAI, ORIAN S.; BARROS, MARIO H.; et al. COQ11 deletion mitigates respiratory deficiency caused by mutations in the gene encoding the coenzyme Q chaperone protein Coq10. Journal of Biological Chemistry, v. 295, n. 18, p. 6023-6042, . (13/09482-8, 13/07937-8)
GUEDES-MONTEIRO, RAQUEL F.; FRANCO, LETICIA V. R.; MODA, BRUNO S.; TZAGOLOFF, ALEXANDER; BARROS, MARIO H.. 5 ` processing of Saccharomyces cerevisiae mitochondrial tRNAs requires expression of multiple genes. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, v. 1866, n. 5, p. 806-818, . (13/09482-8, 17/23921-5, 13/07937-8)
GUEDES-MONTEIRO, RAQUEL FONSECA; FERREIRA-JUNIOR, JOSE RIBAMAR; BLEICHER, LUCAS; NOBREGA, FRANCISCO G.; BARRIENTOS, ANTONI; BARROS, MARIO H.. Mitochondrial ribosome bL34 mutants present diminished translation of cytochrome c oxidase subunits. Cell Biology International, v. 42, n. 6, SI, p. 630-642, . (13/09482-8, 11/18892-0, 13/07937-8)
RIBEIRO FRANCO, LETICIA VELOSO; MODA, BRUNO S.; SOARES, MARIA A. K. M.; BARROS, MARIO H.. Msc6p is required for mitochondrial translation initiation in the absence of formylated Met-tRNA(fMet). FEBS Journal, v. 286, n. 7, p. 1407-1419, . (13/09482-8, 17/23921-5, 13/07937-8, 16/25907-7)

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