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Pharmacological effect of condensed tannins extracted from Stryphnodendron adstringens: action on Candida albicans biofilm, immunomodulatory and antifungal in a murine model of vaginal candidiasis

Grant number: 13/11232-0
Support Opportunities:Regular Research Grants
Duration: October 01, 2013 - September 30, 2015
Field of knowledge:Biological Sciences - Microbiology
Principal Investigator:Kelly Ishida
Grantee:Kelly Ishida
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated researchers:Carlos Pelleschi Taborda ; Celso Vataru Nakamura ; João Carlos Palazzo de Mello ; Sonia Rozental


Vulvovaginal candidiasis (VVC) is one of the most common vaginal infections. 50-75% of women suffer from symptomatic VVC and 5% develop recurrent VVC. Approximately 90% of isolates from vaginal fluid are identified as Candida albicans and the minority of cases is caused by Candida non-albicans. Many antifungal used to treat VVC available have drawbacks such as narrow spectrum of action and toxicity. Although few resistant strains to antifungal agents have been isolated from vaginal fluid, CVV remains a common problem and often in healthy women or immunocompetent. The search for new antifungal agents has been a challenge for researchers, and several medicinal plants have been considered an excellent study. Stryphnodendron adstringens (Mart.) Coville, popularly known as "barbatimão", is a plant commonly found in the central Brazilian savannah. The stem bark of this plant are commonly used as anti-inflammatory and astringent in the treatment of wounds and vaginal infections, and some pharmacological activities been proven scientifically through laboratory tests, including antifungal activity. In 2006, our research group has shown that condensed tannins present in subfraction F2.4 obtained from the stem bark of S. adstringens showed better activity against C. albicans strains isolated from vaginal fluid with similar action of nystatin. The subfraction F2.4 interfered with cell surface hydrophobicity, adhesion, germ tube formation, alteration on the budding and cell wall of C. albicans. The inhibitory effects were attributed to the proanthocyanidin polymer composed by prodelfinidin and prorobinetinidina units and gallic acid residue with a average molecular weight of 2114 Da. To continue the evaluation of the antifungal condensed tannins extracted from bark S. adstringens this project aims to determine the effect of the F2 fraction and subfraction F2.4 on the C. albicans biofilm and to evaluate the therapeutic use of gel formulation containing condensed tannins and immunomodulatory effect in a murine model of vaginal candidiasis. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DUARTE DE FREITAS, ALINE LUIZA; KAPLUM, VANESSA; PEREIRA ROSSI, DIEGO CONRADO; ROQUE DA SILVA, LEANDRO BUFFONI; CARVALHO MELHEM, MARCIA DE SOUZA; TABORDA, CARLOS PELLESCHI; PALAZZO DE MELLO, JOAO CARLOS; NAKAMURA, CELSO VATARU; ISHIDA, KELLY. Proanthocyanidin polymeric tannins from Stryphnodendron adstringens are effective against Candida spp. isolates and for vaginal candidiasis treatment. Journal of Ethnopharmacology, v. 216, p. 184-190, . (16/04816-3, 13/11232-0)
FARIA LUIZ, RAUL LEAL; MACHADO VILA, TAISSA VIEIRA; PALAZZO DE MELLO, JOAO CARLOS; NAKAMURA, CELSO VATARU; ROZENTAL, SONIA; ISHIDA, KELLY. Proanthocyanidins polymeric tannin from Stryphnodendron adstringens are active against Candida albicans biofilms. BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE, v. 15, . (13/11232-0)

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