Molecular characterization of plasmids in Brazilian clinical isolates carrying qnr genes

Abstract

It is very important to know how microorganisms acquire and express resistance phenotype to promote the development of effective strategy for regulating the use of antimicrobial agents commonly used in Brazil. Therefore, the research project was designed to investigate plasmideal mechanisms involved in the maintenance of resistant bacterial strains at the environment. Plasmids carrying qnr determinants previously reported in bacterial strains isolated in Brazil (n = 19) will be typed and sub-typed by Multiplex PCR and Sequencing by multilocus (pMLST), molecular methods that have not been explored in Brazil, which allow to compare data obtained from strains isolated in various parts of the world. The results expand knowledge about types of plasmids involved in these resistance phenotypes, allowing the determination of the epidemiological profile of occurrence of plamideal groups higher frequency. (AU)